Investigating the effects of chronic low-dose radiation exposure in the liver of a hypothermic zebrafish model
Mankind’s quest for a manned mission to Mars is placing increased emphasis on the development of innovative radio-protective countermeasures for long-term space travel. Hibernation confers radio-protective effects in hibernating animals, and this has led to the investigation of synthetic torpor to m...
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ftpubmed:oai:pubmedcentral.nih.gov:9845366 2023-05-15T18:42:07+02:00 Investigating the effects of chronic low-dose radiation exposure in the liver of a hypothermic zebrafish model Cahill, Thomas da Silveira, Willian Abraham Renaud, Ludivine Wang, Hao Williamson, Tucker Chung, Dongjun Chan, Sherine Overton, Ian Hardiman, Gary 2023-01-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845366/ http://www.ncbi.nlm.nih.gov/pubmed/36650199 https://doi.org/10.1038/s41598-022-26976-4 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845366/ http://www.ncbi.nlm.nih.gov/pubmed/36650199 http://dx.doi.org/10.1038/s41598-022-26976-4 © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Sci Rep Article Text 2023 ftpubmed https://doi.org/10.1038/s41598-022-26976-4 2023-01-22T02:04:51Z Mankind’s quest for a manned mission to Mars is placing increased emphasis on the development of innovative radio-protective countermeasures for long-term space travel. Hibernation confers radio-protective effects in hibernating animals, and this has led to the investigation of synthetic torpor to mitigate the deleterious effects of chronic low-dose-rate radiation exposure. Here we describe an induced torpor model we developed using the zebrafish. We explored the effects of radiation exposure on this model with a focus on the liver. Transcriptomic and behavioural analyses were performed. Radiation exposure resulted in transcriptomic perturbations in lipid metabolism and absorption, wound healing, immune response, and fibrogenic pathways. Induced torpor reduced metabolism and increased pro-survival, anti-apoptotic, and DNA repair pathways. Coupled with radiation exposure, induced torpor led to a stress response but also revealed maintenance of DNA repair mechanisms, pro-survival and anti-apoptotic signals. To further characterise our model of induced torpor, the zebrafish model was compared with hepatic transcriptomic data from hibernating grizzly bears (Ursus arctos horribilis) and active controls revealing conserved responses in gene expression associated with anti-apoptotic processes, DNA damage repair, cell survival, proliferation, and antioxidant response. Similarly, the radiation group was compared with space-flown mice revealing shared changes in lipid metabolism. Text Ursus arctos PubMed Central (PMC) Scientific Reports 13 1 |
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Article Cahill, Thomas da Silveira, Willian Abraham Renaud, Ludivine Wang, Hao Williamson, Tucker Chung, Dongjun Chan, Sherine Overton, Ian Hardiman, Gary Investigating the effects of chronic low-dose radiation exposure in the liver of a hypothermic zebrafish model |
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Mankind’s quest for a manned mission to Mars is placing increased emphasis on the development of innovative radio-protective countermeasures for long-term space travel. Hibernation confers radio-protective effects in hibernating animals, and this has led to the investigation of synthetic torpor to mitigate the deleterious effects of chronic low-dose-rate radiation exposure. Here we describe an induced torpor model we developed using the zebrafish. We explored the effects of radiation exposure on this model with a focus on the liver. Transcriptomic and behavioural analyses were performed. Radiation exposure resulted in transcriptomic perturbations in lipid metabolism and absorption, wound healing, immune response, and fibrogenic pathways. Induced torpor reduced metabolism and increased pro-survival, anti-apoptotic, and DNA repair pathways. Coupled with radiation exposure, induced torpor led to a stress response but also revealed maintenance of DNA repair mechanisms, pro-survival and anti-apoptotic signals. To further characterise our model of induced torpor, the zebrafish model was compared with hepatic transcriptomic data from hibernating grizzly bears (Ursus arctos horribilis) and active controls revealing conserved responses in gene expression associated with anti-apoptotic processes, DNA damage repair, cell survival, proliferation, and antioxidant response. Similarly, the radiation group was compared with space-flown mice revealing shared changes in lipid metabolism. |
format |
Text |
author |
Cahill, Thomas da Silveira, Willian Abraham Renaud, Ludivine Wang, Hao Williamson, Tucker Chung, Dongjun Chan, Sherine Overton, Ian Hardiman, Gary |
author_facet |
Cahill, Thomas da Silveira, Willian Abraham Renaud, Ludivine Wang, Hao Williamson, Tucker Chung, Dongjun Chan, Sherine Overton, Ian Hardiman, Gary |
author_sort |
Cahill, Thomas |
title |
Investigating the effects of chronic low-dose radiation exposure in the liver of a hypothermic zebrafish model |
title_short |
Investigating the effects of chronic low-dose radiation exposure in the liver of a hypothermic zebrafish model |
title_full |
Investigating the effects of chronic low-dose radiation exposure in the liver of a hypothermic zebrafish model |
title_fullStr |
Investigating the effects of chronic low-dose radiation exposure in the liver of a hypothermic zebrafish model |
title_full_unstemmed |
Investigating the effects of chronic low-dose radiation exposure in the liver of a hypothermic zebrafish model |
title_sort |
investigating the effects of chronic low-dose radiation exposure in the liver of a hypothermic zebrafish model |
publisher |
Nature Publishing Group UK |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845366/ http://www.ncbi.nlm.nih.gov/pubmed/36650199 https://doi.org/10.1038/s41598-022-26976-4 |
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Ursus arctos |
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Ursus arctos |
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Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845366/ http://www.ncbi.nlm.nih.gov/pubmed/36650199 http://dx.doi.org/10.1038/s41598-022-26976-4 |
op_rights |
© The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-022-26976-4 |
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Scientific Reports |
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13 |
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