CgDM9CP-5-Integrin-MAPK Pathway Regulates the Production of CgIL-17s and Cgdefensins in the Pacific Oyster, Crassostrea gigas
DM9 domain containing protein (DM9CP) is a family of newly identified recognition receptors exiting in most organisms except plants and mammals. In the current study, to our knowledge, a novel DM9CP-5 (CgDM9CP-5) with two tandem DM9 repeats and high expression level in gill was identified from the P...
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ftpubmed:oai:pubmedcentral.nih.gov:9842941 2023-05-15T15:58:19+02:00 CgDM9CP-5-Integrin-MAPK Pathway Regulates the Production of CgIL-17s and Cgdefensins in the Pacific Oyster, Crassostrea gigas Liu, Yu Wang, Weilin Sun, Jiejie Li, Yinan Wu, Shasha Li, Qing Dong, Miren Wang, Lingling Song, Linsheng 2023-02-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842941/ http://www.ncbi.nlm.nih.gov/pubmed/36548464 https://doi.org/10.4049/jimmunol.2200016 en eng AAI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842941/ http://www.ncbi.nlm.nih.gov/pubmed/36548464 http://dx.doi.org/10.4049/jimmunol.2200016 Copyright © 2023 by The American Association of Immunologists, Inc. This article is distributed under The American Association of Immunologists, Inc.,Reuse Terms and Conditions for Author Choice articles. J Immunol Immune Regulation Text 2023 ftpubmed https://doi.org/10.4049/jimmunol.2200016 2023-02-05T01:45:42Z DM9 domain containing protein (DM9CP) is a family of newly identified recognition receptors exiting in most organisms except plants and mammals. In the current study, to our knowledge, a novel DM9CP-5 (CgDM9CP-5) with two tandem DM9 repeats and high expression level in gill was identified from the Pacific oyster, Crassostrea gigas. The deduced amino acid sequence of CgDM9CP-5 shared 62.1% identity with CgDM9CP-1 from C. gigas, and 47.8% identity with OeFAMeT from Ostrea edulis. The recombinant CgDM9CP-5 (rCgDM9CP-5) was able to bind d-mannose, LPS, peptidoglycan, and polyinosinic-polycytidylic acid, as well as fungi Pichia pastoris, Gram-negative bacteria Escherichia coli and Vibrio splendidus, and Gram-positive bacteria Staphylococcus aureus. The mRNA transcript of CgDM9CP-5 was highly expressed in gill, and its protein was mainly distributed in gill mucus. After the stimulations with V. splendidus and mannose, mRNA expression of CgDM9CP-5 in oyster gill was significantly upregulated and reached the peak level at 6 and 24 h, which was 13.58-fold (p < 0.05) and 14.01-fold (p < 0.05) of that in the control group, respectively. CgDM9CP-5 was able to bind CgIntegrin both in vivo and in vitro. After CgDM9CP-5 or CgIntegrin was knocked down by RNA interference, the phosphorylation levels of JNK and P38 in the MAPK pathway decreased, and the expression levels of CgIL-17s (CgIL-17-3, -4, -5, and -6), Cg-Defh1, Cg-Defh2, and CgMolluscidin were significantly downregulated. These results suggested that there was a pathway of DM9CP-5-Integrin-MAPK mediated by CgDM9CP-5 to regulate the release of proinflammatory factors and defensins in C. gigas. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific The Journal of Immunology 210 3 245 258 |
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PubMed Central (PMC) |
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ftpubmed |
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English |
topic |
Immune Regulation |
spellingShingle |
Immune Regulation Liu, Yu Wang, Weilin Sun, Jiejie Li, Yinan Wu, Shasha Li, Qing Dong, Miren Wang, Lingling Song, Linsheng CgDM9CP-5-Integrin-MAPK Pathway Regulates the Production of CgIL-17s and Cgdefensins in the Pacific Oyster, Crassostrea gigas |
topic_facet |
Immune Regulation |
description |
DM9 domain containing protein (DM9CP) is a family of newly identified recognition receptors exiting in most organisms except plants and mammals. In the current study, to our knowledge, a novel DM9CP-5 (CgDM9CP-5) with two tandem DM9 repeats and high expression level in gill was identified from the Pacific oyster, Crassostrea gigas. The deduced amino acid sequence of CgDM9CP-5 shared 62.1% identity with CgDM9CP-1 from C. gigas, and 47.8% identity with OeFAMeT from Ostrea edulis. The recombinant CgDM9CP-5 (rCgDM9CP-5) was able to bind d-mannose, LPS, peptidoglycan, and polyinosinic-polycytidylic acid, as well as fungi Pichia pastoris, Gram-negative bacteria Escherichia coli and Vibrio splendidus, and Gram-positive bacteria Staphylococcus aureus. The mRNA transcript of CgDM9CP-5 was highly expressed in gill, and its protein was mainly distributed in gill mucus. After the stimulations with V. splendidus and mannose, mRNA expression of CgDM9CP-5 in oyster gill was significantly upregulated and reached the peak level at 6 and 24 h, which was 13.58-fold (p < 0.05) and 14.01-fold (p < 0.05) of that in the control group, respectively. CgDM9CP-5 was able to bind CgIntegrin both in vivo and in vitro. After CgDM9CP-5 or CgIntegrin was knocked down by RNA interference, the phosphorylation levels of JNK and P38 in the MAPK pathway decreased, and the expression levels of CgIL-17s (CgIL-17-3, -4, -5, and -6), Cg-Defh1, Cg-Defh2, and CgMolluscidin were significantly downregulated. These results suggested that there was a pathway of DM9CP-5-Integrin-MAPK mediated by CgDM9CP-5 to regulate the release of proinflammatory factors and defensins in C. gigas. |
format |
Text |
author |
Liu, Yu Wang, Weilin Sun, Jiejie Li, Yinan Wu, Shasha Li, Qing Dong, Miren Wang, Lingling Song, Linsheng |
author_facet |
Liu, Yu Wang, Weilin Sun, Jiejie Li, Yinan Wu, Shasha Li, Qing Dong, Miren Wang, Lingling Song, Linsheng |
author_sort |
Liu, Yu |
title |
CgDM9CP-5-Integrin-MAPK Pathway Regulates the Production of CgIL-17s and Cgdefensins in the Pacific Oyster, Crassostrea gigas |
title_short |
CgDM9CP-5-Integrin-MAPK Pathway Regulates the Production of CgIL-17s and Cgdefensins in the Pacific Oyster, Crassostrea gigas |
title_full |
CgDM9CP-5-Integrin-MAPK Pathway Regulates the Production of CgIL-17s and Cgdefensins in the Pacific Oyster, Crassostrea gigas |
title_fullStr |
CgDM9CP-5-Integrin-MAPK Pathway Regulates the Production of CgIL-17s and Cgdefensins in the Pacific Oyster, Crassostrea gigas |
title_full_unstemmed |
CgDM9CP-5-Integrin-MAPK Pathway Regulates the Production of CgIL-17s and Cgdefensins in the Pacific Oyster, Crassostrea gigas |
title_sort |
cgdm9cp-5-integrin-mapk pathway regulates the production of cgil-17s and cgdefensins in the pacific oyster, crassostrea gigas |
publisher |
AAI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842941/ http://www.ncbi.nlm.nih.gov/pubmed/36548464 https://doi.org/10.4049/jimmunol.2200016 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
J Immunol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842941/ http://www.ncbi.nlm.nih.gov/pubmed/36548464 http://dx.doi.org/10.4049/jimmunol.2200016 |
op_rights |
Copyright © 2023 by The American Association of Immunologists, Inc. This article is distributed under The American Association of Immunologists, Inc.,Reuse Terms and Conditions for Author Choice articles. |
op_doi |
https://doi.org/10.4049/jimmunol.2200016 |
container_title |
The Journal of Immunology |
container_volume |
210 |
container_issue |
3 |
container_start_page |
245 |
op_container_end_page |
258 |
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1766394045273210880 |