Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas
BACKGROUND: Lysine-specific demethylase 1 (LSD1) is an essential epigenetic regulator of hematopoietic differentiation, which can specifically mono-methylate H3K4 (H3K4me1) and di-methylate H3K4 (H3K4me2) as a transcriptional corepressor. Previous reports have been suggested that it participated in...
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ftpubmed:oai:pubmedcentral.nih.gov:9797820 2023-05-15T15:58:18+02:00 Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas Gu, Xiaoyu Qiao, Xue Yu, Simiao Song, Xiaorui Wang, Lingling Song, Linsheng 2022-12-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797820/ https://doi.org/10.3389/fimmu.2022.1088149 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797820/ http://dx.doi.org/10.3389/fimmu.2022.1088149 Copyright © 2022 Gu, Qiao, Yu, Song, Wang and Song https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Immunol Immunology Text 2022 ftpubmed https://doi.org/10.3389/fimmu.2022.1088149 2023-01-01T01:53:36Z BACKGROUND: Lysine-specific demethylase 1 (LSD1) is an essential epigenetic regulator of hematopoietic differentiation, which can specifically mono-methylate H3K4 (H3K4me1) and di-methylate H3K4 (H3K4me2) as a transcriptional corepressor. Previous reports have been suggested that it participated in hematopoiesis and embryonic development process. Here, a conserved LSD1 (CgLSD1) with a SWIRM domain and an amino oxidase (AO) domain was identified from the Pacific oyster Crassostrea gigas. METHODS: We conducted a comprehensive analysis by various means to verify the function of CgLSD1 in hematopoietic process, including quantitative real-time PCR (qRT-PCR) analysis, western blot analysis, immunofluorescence assay, RNA interference (RNAi) and flow cytometry. RESULTS: The qRT-PCR analysis revealed that the transcripts of CgLSD1 were widely expressed in oyster tissues with the highest level in the mantle. And the transcripts of CgLSD1 were ubiquitously expressed during larval development with the highest expression level at the early D-veliger larvae stage. In hemocytes after Vibrio splendidus stimulation, the transcripts of CgLSD1 were significantly downregulated at 3, 6, 24, and 48 h with the lowest level at 3 h compared to that in the Seawater group (SW group). Immunocytochemical analysis showed that CgLSD1 was mainly distributed in the nucleus of hemocytes. After the CgLSD1 was knocked down by RNAi, the H3K4me1 and H3K4me2 methylation level significantly increased in hemocyte protein. Besides, the percentage of hemocytes with EdU-positive signals in the total circulating hemocytes significantly increased after V. splendidus stimulation. After RNAi of CgLSD1, the expression of potential granulocyte markers CgSOX11 and CgAATase as well as oyster cytokine-like factor CgAstakine were increased significantly in mRNA level, while the transcripts of potential agranulocyte marker CgCD9 was decreased significantly after V. splendidus stimulation. CONCLUSION: The above results demonstrated that CgLSD1 was a conserved member ... Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Frontiers in Immunology 13 |
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English |
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Immunology |
spellingShingle |
Immunology Gu, Xiaoyu Qiao, Xue Yu, Simiao Song, Xiaorui Wang, Lingling Song, Linsheng Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
topic_facet |
Immunology |
description |
BACKGROUND: Lysine-specific demethylase 1 (LSD1) is an essential epigenetic regulator of hematopoietic differentiation, which can specifically mono-methylate H3K4 (H3K4me1) and di-methylate H3K4 (H3K4me2) as a transcriptional corepressor. Previous reports have been suggested that it participated in hematopoiesis and embryonic development process. Here, a conserved LSD1 (CgLSD1) with a SWIRM domain and an amino oxidase (AO) domain was identified from the Pacific oyster Crassostrea gigas. METHODS: We conducted a comprehensive analysis by various means to verify the function of CgLSD1 in hematopoietic process, including quantitative real-time PCR (qRT-PCR) analysis, western blot analysis, immunofluorescence assay, RNA interference (RNAi) and flow cytometry. RESULTS: The qRT-PCR analysis revealed that the transcripts of CgLSD1 were widely expressed in oyster tissues with the highest level in the mantle. And the transcripts of CgLSD1 were ubiquitously expressed during larval development with the highest expression level at the early D-veliger larvae stage. In hemocytes after Vibrio splendidus stimulation, the transcripts of CgLSD1 were significantly downregulated at 3, 6, 24, and 48 h with the lowest level at 3 h compared to that in the Seawater group (SW group). Immunocytochemical analysis showed that CgLSD1 was mainly distributed in the nucleus of hemocytes. After the CgLSD1 was knocked down by RNAi, the H3K4me1 and H3K4me2 methylation level significantly increased in hemocyte protein. Besides, the percentage of hemocytes with EdU-positive signals in the total circulating hemocytes significantly increased after V. splendidus stimulation. After RNAi of CgLSD1, the expression of potential granulocyte markers CgSOX11 and CgAATase as well as oyster cytokine-like factor CgAstakine were increased significantly in mRNA level, while the transcripts of potential agranulocyte marker CgCD9 was decreased significantly after V. splendidus stimulation. CONCLUSION: The above results demonstrated that CgLSD1 was a conserved member ... |
format |
Text |
author |
Gu, Xiaoyu Qiao, Xue Yu, Simiao Song, Xiaorui Wang, Lingling Song, Linsheng |
author_facet |
Gu, Xiaoyu Qiao, Xue Yu, Simiao Song, Xiaorui Wang, Lingling Song, Linsheng |
author_sort |
Gu, Xiaoyu |
title |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_short |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_full |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_fullStr |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_full_unstemmed |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_sort |
histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster crassostrea gigas |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797820/ https://doi.org/10.3389/fimmu.2022.1088149 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Front Immunol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797820/ http://dx.doi.org/10.3389/fimmu.2022.1088149 |
op_rights |
Copyright © 2022 Gu, Qiao, Yu, Song, Wang and Song https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2022.1088149 |
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Frontiers in Immunology |
container_volume |
13 |
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1766394027504041984 |