First characterization of the parasite Haplosporidium costale in France and development of a real‐time PCR assay for its rapid detection in the Pacific oyster, Crassostrea gigas
The Pacific cupped oyster Crassostrea gigas is one of the most ‘globalized’ marine invertebrates and its production is predominant in many parts of the world including Europe. However, it is threatened by mortality events associated with pathogenic microorganisms such as the virus OsHV‐1 and the bac...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790386/ http://www.ncbi.nlm.nih.gov/pubmed/35353448 https://doi.org/10.1111/tbed.14541 |
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ftpubmed:oai:pubmedcentral.nih.gov:9790386 2023-05-15T15:58:28+02:00 First characterization of the parasite Haplosporidium costale in France and development of a real‐time PCR assay for its rapid detection in the Pacific oyster, Crassostrea gigas Arzul, Isabelle Garcia, Céline Chollet, Bruno Serpin, Delphine Lupo, Coralie Noyer, Mathilde Tourbiez, Delphine Berland, Chloé Dégremont, Lionel Travers, Marie‐Agnès 2022-04-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790386/ http://www.ncbi.nlm.nih.gov/pubmed/35353448 https://doi.org/10.1111/tbed.14541 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790386/ http://www.ncbi.nlm.nih.gov/pubmed/35353448 http://dx.doi.org/10.1111/tbed.14541 © 2022 The Authors. Transboundary and Emerging Diseases published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. CC-BY-NC-ND Transbound Emerg Dis Original Articles Text 2022 ftpubmed https://doi.org/10.1111/tbed.14541 2023-01-01T01:38:38Z The Pacific cupped oyster Crassostrea gigas is one of the most ‘globalized’ marine invertebrates and its production is predominant in many parts of the world including Europe. However, it is threatened by mortality events associated with pathogenic microorganisms such as the virus OsHV‐1 and the bacteria Vibrio aestuarianus. C. gigas is also a host for protozoan parasites including haplosporidians. In contrast with Haplosporidium nelsoni previously detected in Europe, H. costale was considered exotic although its presence in French oysters was suggested in the 1980s based on ultrastructural examination. Here, a combination of light and transmission electron microscopy, PCR and sequencing allowed characterizing the presence of the parasite in the context of low mortality events which occurred in 2019 in France. Histological observation revealed the presence of uninucleated, plasmodial and spore stages within the connective tissues of some oysters. Ultrastructural features were similar to H. costale ones in particular the presence of axe‐shaped haplosporosomes in spore cytoplasms. Three fragments of the genome including partial small subunit rRNA gene, the ITS‐1, 5.8S and ITS‐2 array and part of the actin gene were successfully sequenced and grouped with H. costale homologous sequences. This is the first time that the presence of H. costale was confirmed in C. gigas in France. Furthermore, a TaqMan real‐time PCR assay was developed and validated [DSe = 92.6% (78.2–99.8) and DSp = 95.5% (92.3–98.6)] to enable the rapid and specific detection of the parasite. The application of the PCR assay on archived samples revealed that the parasite has been present in French oyster populations at least since 2008. Considering the little information available on this parasite, the newly developed TaqMan assay will be very helpful to investigate the temporal and geographic distribution and the life cycle of the parasite in France and more generally in C. gigas geographic range. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Transboundary and Emerging Diseases 69 5 |
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Original Articles |
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Original Articles Arzul, Isabelle Garcia, Céline Chollet, Bruno Serpin, Delphine Lupo, Coralie Noyer, Mathilde Tourbiez, Delphine Berland, Chloé Dégremont, Lionel Travers, Marie‐Agnès First characterization of the parasite Haplosporidium costale in France and development of a real‐time PCR assay for its rapid detection in the Pacific oyster, Crassostrea gigas |
topic_facet |
Original Articles |
description |
The Pacific cupped oyster Crassostrea gigas is one of the most ‘globalized’ marine invertebrates and its production is predominant in many parts of the world including Europe. However, it is threatened by mortality events associated with pathogenic microorganisms such as the virus OsHV‐1 and the bacteria Vibrio aestuarianus. C. gigas is also a host for protozoan parasites including haplosporidians. In contrast with Haplosporidium nelsoni previously detected in Europe, H. costale was considered exotic although its presence in French oysters was suggested in the 1980s based on ultrastructural examination. Here, a combination of light and transmission electron microscopy, PCR and sequencing allowed characterizing the presence of the parasite in the context of low mortality events which occurred in 2019 in France. Histological observation revealed the presence of uninucleated, plasmodial and spore stages within the connective tissues of some oysters. Ultrastructural features were similar to H. costale ones in particular the presence of axe‐shaped haplosporosomes in spore cytoplasms. Three fragments of the genome including partial small subunit rRNA gene, the ITS‐1, 5.8S and ITS‐2 array and part of the actin gene were successfully sequenced and grouped with H. costale homologous sequences. This is the first time that the presence of H. costale was confirmed in C. gigas in France. Furthermore, a TaqMan real‐time PCR assay was developed and validated [DSe = 92.6% (78.2–99.8) and DSp = 95.5% (92.3–98.6)] to enable the rapid and specific detection of the parasite. The application of the PCR assay on archived samples revealed that the parasite has been present in French oyster populations at least since 2008. Considering the little information available on this parasite, the newly developed TaqMan assay will be very helpful to investigate the temporal and geographic distribution and the life cycle of the parasite in France and more generally in C. gigas geographic range. |
format |
Text |
author |
Arzul, Isabelle Garcia, Céline Chollet, Bruno Serpin, Delphine Lupo, Coralie Noyer, Mathilde Tourbiez, Delphine Berland, Chloé Dégremont, Lionel Travers, Marie‐Agnès |
author_facet |
Arzul, Isabelle Garcia, Céline Chollet, Bruno Serpin, Delphine Lupo, Coralie Noyer, Mathilde Tourbiez, Delphine Berland, Chloé Dégremont, Lionel Travers, Marie‐Agnès |
author_sort |
Arzul, Isabelle |
title |
First characterization of the parasite Haplosporidium costale in France and development of a real‐time PCR assay for its rapid detection in the Pacific oyster, Crassostrea gigas |
title_short |
First characterization of the parasite Haplosporidium costale in France and development of a real‐time PCR assay for its rapid detection in the Pacific oyster, Crassostrea gigas |
title_full |
First characterization of the parasite Haplosporidium costale in France and development of a real‐time PCR assay for its rapid detection in the Pacific oyster, Crassostrea gigas |
title_fullStr |
First characterization of the parasite Haplosporidium costale in France and development of a real‐time PCR assay for its rapid detection in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
First characterization of the parasite Haplosporidium costale in France and development of a real‐time PCR assay for its rapid detection in the Pacific oyster, Crassostrea gigas |
title_sort |
first characterization of the parasite haplosporidium costale in france and development of a real‐time pcr assay for its rapid detection in the pacific oyster, crassostrea gigas |
publisher |
John Wiley and Sons Inc. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790386/ http://www.ncbi.nlm.nih.gov/pubmed/35353448 https://doi.org/10.1111/tbed.14541 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Transbound Emerg Dis |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790386/ http://www.ncbi.nlm.nih.gov/pubmed/35353448 http://dx.doi.org/10.1111/tbed.14541 |
op_rights |
© 2022 The Authors. Transboundary and Emerging Diseases published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1111/tbed.14541 |
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Transboundary and Emerging Diseases |
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69 |
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5 |
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1766394211485089792 |