Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters (Crassostrea gigas)
Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V. parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V. parahaemolyticus accumulation and depuration in adult Cr...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790381/ http://www.ncbi.nlm.nih.gov/pubmed/35384247 https://doi.org/10.1111/1462-2920.15996 |
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ftpubmed:oai:pubmedcentral.nih.gov:9790381 2023-05-15T15:57:43+02:00 Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters (Crassostrea gigas) Sorée, Marion Delavat, François Lambert, Christophe Lozach, Solen Papin, Mathias Petton, Bruno Passerini, Delphine Dégremont, Lionel Hervio Heath, Dominique 2022-05-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790381/ http://www.ncbi.nlm.nih.gov/pubmed/35384247 https://doi.org/10.1111/1462-2920.15996 en eng John Wiley & Sons, Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790381/ http://www.ncbi.nlm.nih.gov/pubmed/35384247 http://dx.doi.org/10.1111/1462-2920.15996 © 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. CC-BY-NC-ND Environ Microbiol Special Issue on Pathogen and Antimicrobial Resistance Ecology Text 2022 ftpubmed https://doi.org/10.1111/1462-2920.15996 2023-01-01T01:38:28Z Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V. parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V. parahaemolyticus accumulation and depuration in adult Crassostrea gigas, we developed a GFP‐tagged V. parahaemolyticus strain (IFVp201‐gfp (+)), as well as a rapid and efficient quantification method in C. gigas oysters haemolymph by flow cytometry. Impact of the life history of C. gigas on accumulation and depuration of V. parahaemolyticus IFVp201 was subsequently investigated. We found that naive oysters, i.e. grown in controlled facilities with UV treated seawater, accumulated significantly more IFVp201 than environmental oysters, i.e. grown in intertidal environment. We hypothesized that environmental oysters could have been immune primed, thus could limit V. parahaemolyticus accumulation. Meanwhile, both naive and environmental oysters had similar depuration rates. Text Crassostrea gigas PubMed Central (PMC) Pacific Environmental Microbiology 24 9 4401 4410 |
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PubMed Central (PMC) |
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English |
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Special Issue on Pathogen and Antimicrobial Resistance Ecology |
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Special Issue on Pathogen and Antimicrobial Resistance Ecology Sorée, Marion Delavat, François Lambert, Christophe Lozach, Solen Papin, Mathias Petton, Bruno Passerini, Delphine Dégremont, Lionel Hervio Heath, Dominique Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters (Crassostrea gigas) |
topic_facet |
Special Issue on Pathogen and Antimicrobial Resistance Ecology |
description |
Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V. parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V. parahaemolyticus accumulation and depuration in adult Crassostrea gigas, we developed a GFP‐tagged V. parahaemolyticus strain (IFVp201‐gfp (+)), as well as a rapid and efficient quantification method in C. gigas oysters haemolymph by flow cytometry. Impact of the life history of C. gigas on accumulation and depuration of V. parahaemolyticus IFVp201 was subsequently investigated. We found that naive oysters, i.e. grown in controlled facilities with UV treated seawater, accumulated significantly more IFVp201 than environmental oysters, i.e. grown in intertidal environment. We hypothesized that environmental oysters could have been immune primed, thus could limit V. parahaemolyticus accumulation. Meanwhile, both naive and environmental oysters had similar depuration rates. |
format |
Text |
author |
Sorée, Marion Delavat, François Lambert, Christophe Lozach, Solen Papin, Mathias Petton, Bruno Passerini, Delphine Dégremont, Lionel Hervio Heath, Dominique |
author_facet |
Sorée, Marion Delavat, François Lambert, Christophe Lozach, Solen Papin, Mathias Petton, Bruno Passerini, Delphine Dégremont, Lionel Hervio Heath, Dominique |
author_sort |
Sorée, Marion |
title |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters (Crassostrea gigas) |
title_short |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters (Crassostrea gigas) |
title_full |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters (Crassostrea gigas) |
title_fullStr |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters (Crassostrea gigas) |
title_full_unstemmed |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters (Crassostrea gigas) |
title_sort |
life history of oysters influences vibrio parahaemolyticus accumulation in pacific oysters (crassostrea gigas) |
publisher |
John Wiley & Sons, Inc. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790381/ http://www.ncbi.nlm.nih.gov/pubmed/35384247 https://doi.org/10.1111/1462-2920.15996 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Environ Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790381/ http://www.ncbi.nlm.nih.gov/pubmed/35384247 http://dx.doi.org/10.1111/1462-2920.15996 |
op_rights |
© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1111/1462-2920.15996 |
container_title |
Environmental Microbiology |
container_volume |
24 |
container_issue |
9 |
container_start_page |
4401 |
op_container_end_page |
4410 |
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1766393412823547904 |