Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas
For over a decade, Pacific oyster mortality syndrome (POMS), a polymicrobial disease, induced recurring episodes of massive mortality affecting Crassostrea gigas oysters worldwide. Recent studies evidenced a combined infection of the ostreid herpesvirus (OsHV-1 μVar) and opportunistic bacteria in af...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769987/ http://www.ncbi.nlm.nih.gov/pubmed/36314927 https://doi.org/10.1128/spectrum.01959-22 |
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ftpubmed:oai:pubmedcentral.nih.gov:9769987 2023-05-15T15:57:55+02:00 Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas Delisle, Lizenn Laroche, Olivier Hilton, Zoë Burguin, Jean-François Rolton, Anne Berry, Jolene Pochon, Xavier Boudry, Pierre Vignier, Julien 2022-10-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769987/ http://www.ncbi.nlm.nih.gov/pubmed/36314927 https://doi.org/10.1128/spectrum.01959-22 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769987/ http://www.ncbi.nlm.nih.gov/pubmed/36314927 http://dx.doi.org/10.1128/spectrum.01959-22 Copyright © 2022 Delisle et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . CC-BY Microbiol Spectr Research Article Text 2022 ftpubmed https://doi.org/10.1128/spectrum.01959-22 2022-12-25T02:04:19Z For over a decade, Pacific oyster mortality syndrome (POMS), a polymicrobial disease, induced recurring episodes of massive mortality affecting Crassostrea gigas oysters worldwide. Recent studies evidenced a combined infection of the ostreid herpesvirus (OsHV-1 μVar) and opportunistic bacteria in affected oysters. However, the role of the oyster microbiota in POMS is not fully understood. While some bacteria can protect hosts from infection, even minor changes to the microbial communities may also facilitate infection and worsen disease severity. Using a laboratory-based experimental infection model, we challenged juveniles from 10 biparental oyster families with previously established contrasted genetically based ability to survive POMS in the field. Combining molecular analyses and 16S rRNA gene sequencing with histopathological observations, we described the temporal kinetics of POMS and characterized the changes in microbiota during infection. By associating the microbiota composition with oyster mortality rate, viral load, and viral gene expression, we were able to identify both potentially harmful and beneficial bacterial amplicon sequence variants (ASVs). We also observed a delay in viral infection resulting in a later onset of mortality in oysters compared to previous observations and a lack of evidence of fatal dysbiosis in infected oysters. Overall, these results provide new insights into how the oyster microbiome may influence POMS disease outcomes and open new perspectives on the use of microbiome composition as a complementary screening tool to determine shellfish health and potentially predict oyster vulnerability to POMS. IMPORTANCE For more than a decade, Pacific oyster mortality syndrome (POMS) has severely impacted the Crassostrea gigas aquaculture industry, at times killing up to 100% of young farmed Pacific oysters, a key commercial species that is cultivated globally. These disease outbreaks have caused major financial losses for the oyster aquaculture industry. Selective breeding has ... Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Microbiology Spectrum 10 6 |
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Research Article Delisle, Lizenn Laroche, Olivier Hilton, Zoë Burguin, Jean-François Rolton, Anne Berry, Jolene Pochon, Xavier Boudry, Pierre Vignier, Julien Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
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Research Article |
description |
For over a decade, Pacific oyster mortality syndrome (POMS), a polymicrobial disease, induced recurring episodes of massive mortality affecting Crassostrea gigas oysters worldwide. Recent studies evidenced a combined infection of the ostreid herpesvirus (OsHV-1 μVar) and opportunistic bacteria in affected oysters. However, the role of the oyster microbiota in POMS is not fully understood. While some bacteria can protect hosts from infection, even minor changes to the microbial communities may also facilitate infection and worsen disease severity. Using a laboratory-based experimental infection model, we challenged juveniles from 10 biparental oyster families with previously established contrasted genetically based ability to survive POMS in the field. Combining molecular analyses and 16S rRNA gene sequencing with histopathological observations, we described the temporal kinetics of POMS and characterized the changes in microbiota during infection. By associating the microbiota composition with oyster mortality rate, viral load, and viral gene expression, we were able to identify both potentially harmful and beneficial bacterial amplicon sequence variants (ASVs). We also observed a delay in viral infection resulting in a later onset of mortality in oysters compared to previous observations and a lack of evidence of fatal dysbiosis in infected oysters. Overall, these results provide new insights into how the oyster microbiome may influence POMS disease outcomes and open new perspectives on the use of microbiome composition as a complementary screening tool to determine shellfish health and potentially predict oyster vulnerability to POMS. IMPORTANCE For more than a decade, Pacific oyster mortality syndrome (POMS) has severely impacted the Crassostrea gigas aquaculture industry, at times killing up to 100% of young farmed Pacific oysters, a key commercial species that is cultivated globally. These disease outbreaks have caused major financial losses for the oyster aquaculture industry. Selective breeding has ... |
format |
Text |
author |
Delisle, Lizenn Laroche, Olivier Hilton, Zoë Burguin, Jean-François Rolton, Anne Berry, Jolene Pochon, Xavier Boudry, Pierre Vignier, Julien |
author_facet |
Delisle, Lizenn Laroche, Olivier Hilton, Zoë Burguin, Jean-François Rolton, Anne Berry, Jolene Pochon, Xavier Boudry, Pierre Vignier, Julien |
author_sort |
Delisle, Lizenn |
title |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_short |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_full |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_fullStr |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_full_unstemmed |
Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas |
title_sort |
understanding the dynamic of poms infection and the role of microbiota composition in the survival of pacific oysters, crassostrea gigas |
publisher |
American Society for Microbiology |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769987/ http://www.ncbi.nlm.nih.gov/pubmed/36314927 https://doi.org/10.1128/spectrum.01959-22 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Microbiol Spectr |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769987/ http://www.ncbi.nlm.nih.gov/pubmed/36314927 http://dx.doi.org/10.1128/spectrum.01959-22 |
op_rights |
Copyright © 2022 Delisle et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
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CC-BY |
op_doi |
https://doi.org/10.1128/spectrum.01959-22 |
container_title |
Microbiology Spectrum |
container_volume |
10 |
container_issue |
6 |
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1766393622097297408 |