Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin
Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3–10,...
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ftpubmed:oai:pubmedcentral.nih.gov:9686691 2023-05-15T13:09:24+02:00 Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin Lee, Ju Eun Noh, Sang-Kyu Kim, Mi Jeong 2022-10-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686691/ http://www.ncbi.nlm.nih.gov/pubmed/36358484 https://doi.org/10.3390/antiox11112112 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686691/ http://www.ncbi.nlm.nih.gov/pubmed/36358484 http://dx.doi.org/10.3390/antiox11112112 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Antioxidants (Basel) Article Text 2022 ftpubmed https://doi.org/10.3390/antiox11112112 2022-11-27T02:01:44Z Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3–10, 10–30, and ≥30 kDa. Each fraction was evaluated for biological activity and structural properties. All fractions contained high levels of glycine and proline. The ≤3 kDa fraction of control and ultrasound-assisted extracts exhibited the highest antioxidant activity as measured using Trolox equivalent antioxidant capacity, ferric ion reducing antioxidant power, oxygen radical absorbance capacity, and an assay on the inhibition of nitric oxide production by LPS-induced macrophages. The structurally digested collagen was evaluated using FTIR spectra and SDS-PAGE after Alcalase(®) and ultrasound treatments. The microstructure of collagen hydrolysate was assessed using SEM microscopy; the surface morphology was altered according to fraction size and extraction conditions. Overall, it was determined that enzyme treatment in combination with ultrasound is the most effective procedure for obtaining digested collagen hydrolysate, which could be used to further improve biotechnological processing for the addition of value to marine production chains in the future. Text alaska pollock Theragra chalcogramma Alaska PubMed Central (PMC) Antioxidants 11 11 2112 |
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Article Lee, Ju Eun Noh, Sang-Kyu Kim, Mi Jeong Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
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Article |
description |
Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3–10, 10–30, and ≥30 kDa. Each fraction was evaluated for biological activity and structural properties. All fractions contained high levels of glycine and proline. The ≤3 kDa fraction of control and ultrasound-assisted extracts exhibited the highest antioxidant activity as measured using Trolox equivalent antioxidant capacity, ferric ion reducing antioxidant power, oxygen radical absorbance capacity, and an assay on the inhibition of nitric oxide production by LPS-induced macrophages. The structurally digested collagen was evaluated using FTIR spectra and SDS-PAGE after Alcalase(®) and ultrasound treatments. The microstructure of collagen hydrolysate was assessed using SEM microscopy; the surface morphology was altered according to fraction size and extraction conditions. Overall, it was determined that enzyme treatment in combination with ultrasound is the most effective procedure for obtaining digested collagen hydrolysate, which could be used to further improve biotechnological processing for the addition of value to marine production chains in the future. |
format |
Text |
author |
Lee, Ju Eun Noh, Sang-Kyu Kim, Mi Jeong |
author_facet |
Lee, Ju Eun Noh, Sang-Kyu Kim, Mi Jeong |
author_sort |
Lee, Ju Eun |
title |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_short |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_full |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_fullStr |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_full_unstemmed |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack (Theragra chalcogramma) Skin |
title_sort |
effects of enzymatic- and ultrasound-assisted extraction on physicochemical and antioxidant properties of collagen hydrolysate fractions from alaska pollack (theragra chalcogramma) skin |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686691/ http://www.ncbi.nlm.nih.gov/pubmed/36358484 https://doi.org/10.3390/antiox11112112 |
genre |
alaska pollock Theragra chalcogramma Alaska |
genre_facet |
alaska pollock Theragra chalcogramma Alaska |
op_source |
Antioxidants (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686691/ http://www.ncbi.nlm.nih.gov/pubmed/36358484 http://dx.doi.org/10.3390/antiox11112112 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/antiox11112112 |
container_title |
Antioxidants |
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11 |
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11 |
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2112 |
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1766175529825730560 |