Impacts of Seawater pH Buffering on the Larval Microbiome and Carry-Over Effects on Later-Life Disease Susceptibility in Pacific Oysters
Ocean acidification upwelling events and the resulting lowered aragonite saturation state of seawater have been linked to high mortality of marine bivalve larvae in hatcheries. Major oyster seed producers along North America’s west coast have mitigated impacts via seawater pH buffering (e.g., additi...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680617/ http://www.ncbi.nlm.nih.gov/pubmed/36342150 https://doi.org/10.1128/aem.01654-22 |
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ftpubmed:oai:pubmedcentral.nih.gov:9680617 2023-05-15T15:59:02+02:00 Impacts of Seawater pH Buffering on the Larval Microbiome and Carry-Over Effects on Later-Life Disease Susceptibility in Pacific Oysters Mackenzie, Clara L. Pearce, Christopher M. Leduc, Sarah Roth, Daniel Kellogg, Colleen T. E. Clemente-Carvalho, Rute B. G. Green, Timothy J. 2022-11-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680617/ http://www.ncbi.nlm.nih.gov/pubmed/36342150 https://doi.org/10.1128/aem.01654-22 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680617/ http://www.ncbi.nlm.nih.gov/pubmed/36342150 http://dx.doi.org/10.1128/aem.01654-22 © Crown copyright 2022. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . CC-BY Appl Environ Microbiol Invertebrate Microbiology Text 2022 ftpubmed https://doi.org/10.1128/aem.01654-22 2022-11-27T01:52:01Z Ocean acidification upwelling events and the resulting lowered aragonite saturation state of seawater have been linked to high mortality of marine bivalve larvae in hatcheries. Major oyster seed producers along North America’s west coast have mitigated impacts via seawater pH buffering (e.g., addition of soda ash). However, little consideration has been given to whether such practice may impact the larval microbiome, with potential carry-over effects on immune competency and disease susceptibility in later-life stages. To investigate possible impacts, Pacific oysters (Crassostrea gigas) were reared under soda ash pH buffered or ambient pH seawater conditions for the first 24 h of development. Both treatment groups were then reared under ambient pH conditions for the remainder of the developmental period. Larval microbiome, immune status (via gene expression), growth, and survival were assessed throughout the developmental period. Juveniles and adults arising from the larval run were then subjected to laboratory-based disease challenges to investigate carry-over effects. Larvae reared under buffered conditions showed an altered microbiome, which was still evident in juvenile animals. Moreover, reduced survival was observed in both juveniles and adults of the buffered group under a simulated marine heatwave and Vibrio exposure compared with those reared under ambient conditions. Results suggest that soda ash pH buffering during early development may compromise later-life stages under stressor conditions, and illustrate the importance of a long-view approach with regard to hatchery husbandry practices and climate change mitigation. IMPORTANCE Shellfish industries are threatened worldwide by recurrent summer mortality events. Such incidences are often associated with Vibrio disease outbreaks, and thus, it is critical that animals are able to mount sufficient immune responses. The oyster immune system is linked to the microbiome which is laid down during early developmental stages. Consequently, shellfish hatcheries ... Text Crassostrea gigas Ocean acidification PubMed Central (PMC) Pacific Applied and Environmental Microbiology 88 22 |
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Invertebrate Microbiology |
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Invertebrate Microbiology Mackenzie, Clara L. Pearce, Christopher M. Leduc, Sarah Roth, Daniel Kellogg, Colleen T. E. Clemente-Carvalho, Rute B. G. Green, Timothy J. Impacts of Seawater pH Buffering on the Larval Microbiome and Carry-Over Effects on Later-Life Disease Susceptibility in Pacific Oysters |
topic_facet |
Invertebrate Microbiology |
description |
Ocean acidification upwelling events and the resulting lowered aragonite saturation state of seawater have been linked to high mortality of marine bivalve larvae in hatcheries. Major oyster seed producers along North America’s west coast have mitigated impacts via seawater pH buffering (e.g., addition of soda ash). However, little consideration has been given to whether such practice may impact the larval microbiome, with potential carry-over effects on immune competency and disease susceptibility in later-life stages. To investigate possible impacts, Pacific oysters (Crassostrea gigas) were reared under soda ash pH buffered or ambient pH seawater conditions for the first 24 h of development. Both treatment groups were then reared under ambient pH conditions for the remainder of the developmental period. Larval microbiome, immune status (via gene expression), growth, and survival were assessed throughout the developmental period. Juveniles and adults arising from the larval run were then subjected to laboratory-based disease challenges to investigate carry-over effects. Larvae reared under buffered conditions showed an altered microbiome, which was still evident in juvenile animals. Moreover, reduced survival was observed in both juveniles and adults of the buffered group under a simulated marine heatwave and Vibrio exposure compared with those reared under ambient conditions. Results suggest that soda ash pH buffering during early development may compromise later-life stages under stressor conditions, and illustrate the importance of a long-view approach with regard to hatchery husbandry practices and climate change mitigation. IMPORTANCE Shellfish industries are threatened worldwide by recurrent summer mortality events. Such incidences are often associated with Vibrio disease outbreaks, and thus, it is critical that animals are able to mount sufficient immune responses. The oyster immune system is linked to the microbiome which is laid down during early developmental stages. Consequently, shellfish hatcheries ... |
format |
Text |
author |
Mackenzie, Clara L. Pearce, Christopher M. Leduc, Sarah Roth, Daniel Kellogg, Colleen T. E. Clemente-Carvalho, Rute B. G. Green, Timothy J. |
author_facet |
Mackenzie, Clara L. Pearce, Christopher M. Leduc, Sarah Roth, Daniel Kellogg, Colleen T. E. Clemente-Carvalho, Rute B. G. Green, Timothy J. |
author_sort |
Mackenzie, Clara L. |
title |
Impacts of Seawater pH Buffering on the Larval Microbiome and Carry-Over Effects on Later-Life Disease Susceptibility in Pacific Oysters |
title_short |
Impacts of Seawater pH Buffering on the Larval Microbiome and Carry-Over Effects on Later-Life Disease Susceptibility in Pacific Oysters |
title_full |
Impacts of Seawater pH Buffering on the Larval Microbiome and Carry-Over Effects on Later-Life Disease Susceptibility in Pacific Oysters |
title_fullStr |
Impacts of Seawater pH Buffering on the Larval Microbiome and Carry-Over Effects on Later-Life Disease Susceptibility in Pacific Oysters |
title_full_unstemmed |
Impacts of Seawater pH Buffering on the Larval Microbiome and Carry-Over Effects on Later-Life Disease Susceptibility in Pacific Oysters |
title_sort |
impacts of seawater ph buffering on the larval microbiome and carry-over effects on later-life disease susceptibility in pacific oysters |
publisher |
American Society for Microbiology |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680617/ http://www.ncbi.nlm.nih.gov/pubmed/36342150 https://doi.org/10.1128/aem.01654-22 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Ocean acidification |
genre_facet |
Crassostrea gigas Ocean acidification |
op_source |
Appl Environ Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680617/ http://www.ncbi.nlm.nih.gov/pubmed/36342150 http://dx.doi.org/10.1128/aem.01654-22 |
op_rights |
© Crown copyright 2022. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1128/aem.01654-22 |
container_title |
Applied and Environmental Microbiology |
container_volume |
88 |
container_issue |
22 |
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1766394810276511744 |