Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific Oyster Crassostrea gigas
Retinoic acid (RA) plays important roles in various biological processes in animals. RA signaling is mediated by two types of nuclear receptors, namely retinoic acid receptor (RAR) and retinoid x receptor (RXR), which regulate gene expression by binding to retinoic acid response elements (RAREs) in...
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ftpubmed:oai:pubmedcentral.nih.gov:9656583 2023-05-15T15:58:48+02:00 Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific Oyster Crassostrea gigas Jin, Qianqian Huo, Chuncao Yang, Wenhao Jin, Kaidi Cai, Shuai Zheng, Yanxin Huang, Baoyu Wei, Lei Zhang, Meiwei Han, Yijing Zhang, Xuekai Liu, Yaqiong Wang, Xiaotong 2022-10-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656583/ http://www.ncbi.nlm.nih.gov/pubmed/36361629 https://doi.org/10.3390/ijms232112840 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656583/ http://www.ncbi.nlm.nih.gov/pubmed/36361629 http://dx.doi.org/10.3390/ijms232112840 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Int J Mol Sci Communication Text 2022 ftpubmed https://doi.org/10.3390/ijms232112840 2022-11-20T02:34:23Z Retinoic acid (RA) plays important roles in various biological processes in animals. RA signaling is mediated by two types of nuclear receptors, namely retinoic acid receptor (RAR) and retinoid x receptor (RXR), which regulate gene expression by binding to retinoic acid response elements (RAREs) in the promoters of target genes. Here, we explored the effect of all-trans retinoic acid (ATRA) on the Pacific oyster Crassostera gigas at the transcriptome level. A total of 586 differentially expressed genes (DEGs) were identified in C. gigas upon ATRA treatment, with 309 upregulated and 277 downregulated genes. Bioinformatic analysis revealed that ATRA affects the development, metabolism, reproduction, and immunity of C. gigas. Four tyrosinase genes, including Tyr-6 (LOC105331209), Tyr-9 (LOC105346503), Tyr-20 (LOC105330910), and Tyr-12 (LOC105320007), were upregulated by ATRA according to the transcriptome data and these results were verified by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. In addition, increased expression of Tyr (a melanin-related TYR gene in C. gigas) and Tyr-2 were detected after ATRA treatment. The yeast one-hybrid assay revealed the DNA-binding activity of the RA receptors CgRAR and CgRXR, and the interaction of CgRAR with RARE present in the Tyr-2 promoter. These results provide evidence for the further studies on the role of ATRA and the mechanism of RA receptors in mollusks. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific International Journal of Molecular Sciences 23 21 12840 |
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Communication |
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Communication Jin, Qianqian Huo, Chuncao Yang, Wenhao Jin, Kaidi Cai, Shuai Zheng, Yanxin Huang, Baoyu Wei, Lei Zhang, Meiwei Han, Yijing Zhang, Xuekai Liu, Yaqiong Wang, Xiaotong Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific Oyster Crassostrea gigas |
topic_facet |
Communication |
description |
Retinoic acid (RA) plays important roles in various biological processes in animals. RA signaling is mediated by two types of nuclear receptors, namely retinoic acid receptor (RAR) and retinoid x receptor (RXR), which regulate gene expression by binding to retinoic acid response elements (RAREs) in the promoters of target genes. Here, we explored the effect of all-trans retinoic acid (ATRA) on the Pacific oyster Crassostera gigas at the transcriptome level. A total of 586 differentially expressed genes (DEGs) were identified in C. gigas upon ATRA treatment, with 309 upregulated and 277 downregulated genes. Bioinformatic analysis revealed that ATRA affects the development, metabolism, reproduction, and immunity of C. gigas. Four tyrosinase genes, including Tyr-6 (LOC105331209), Tyr-9 (LOC105346503), Tyr-20 (LOC105330910), and Tyr-12 (LOC105320007), were upregulated by ATRA according to the transcriptome data and these results were verified by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. In addition, increased expression of Tyr (a melanin-related TYR gene in C. gigas) and Tyr-2 were detected after ATRA treatment. The yeast one-hybrid assay revealed the DNA-binding activity of the RA receptors CgRAR and CgRXR, and the interaction of CgRAR with RARE present in the Tyr-2 promoter. These results provide evidence for the further studies on the role of ATRA and the mechanism of RA receptors in mollusks. |
format |
Text |
author |
Jin, Qianqian Huo, Chuncao Yang, Wenhao Jin, Kaidi Cai, Shuai Zheng, Yanxin Huang, Baoyu Wei, Lei Zhang, Meiwei Han, Yijing Zhang, Xuekai Liu, Yaqiong Wang, Xiaotong |
author_facet |
Jin, Qianqian Huo, Chuncao Yang, Wenhao Jin, Kaidi Cai, Shuai Zheng, Yanxin Huang, Baoyu Wei, Lei Zhang, Meiwei Han, Yijing Zhang, Xuekai Liu, Yaqiong Wang, Xiaotong |
author_sort |
Jin, Qianqian |
title |
Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific Oyster Crassostrea gigas |
title_short |
Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific Oyster Crassostrea gigas |
title_full |
Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific Oyster Crassostrea gigas |
title_fullStr |
Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific Oyster Crassostrea gigas |
title_sort |
regulation of tyrosinase gene expression by retinoic acid pathway in the pacific oyster crassostrea gigas |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656583/ http://www.ncbi.nlm.nih.gov/pubmed/36361629 https://doi.org/10.3390/ijms232112840 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Int J Mol Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656583/ http://www.ncbi.nlm.nih.gov/pubmed/36361629 http://dx.doi.org/10.3390/ijms232112840 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/ijms232112840 |
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International Journal of Molecular Sciences |
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23 |
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21 |
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12840 |
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1766394566091472896 |