Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment
ABSTRACT: A synergistic approach using cultivation methods, chemical, and bioinformatic analyses was applied to explore the potential of Pseudoalteromonas sp. S8-8 in the production of extracellular polymeric substances (EPSs) and the possible physiological traits related to heavy metal and/or antib...
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ftpubmed:oai:pubmedcentral.nih.gov:9592659 2023-05-15T13:51:05+02:00 Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment Rizzo, Carmen Perrin, Elena Poli, Annarita Finore, Ilaria Fani, Renato Lo Giudice, Angelina 2022-09-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592659/ http://www.ncbi.nlm.nih.gov/pubmed/36156161 https://doi.org/10.1007/s00253-022-12180-x en eng Springer Berlin Heidelberg http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592659/ http://www.ncbi.nlm.nih.gov/pubmed/36156161 http://dx.doi.org/10.1007/s00253-022-12180-x © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Appl Microbiol Biotechnol Applied Microbial and Cell Physiology Text 2022 ftpubmed https://doi.org/10.1007/s00253-022-12180-x 2022-10-30T00:46:43Z ABSTRACT: A synergistic approach using cultivation methods, chemical, and bioinformatic analyses was applied to explore the potential of Pseudoalteromonas sp. S8-8 in the production of extracellular polymeric substances (EPSs) and the possible physiological traits related to heavy metal and/or antibiotic resistance. The effects of different parameters (carbon source, carbon source concentration, temperature, pH and NaCl supplement) were tested to ensure the optimization of growth conditions for EPS production by the strain S8-8. The highest yield of EPS was obtained during growth in culture medium supplemented with glucose (final concentration 2%) and NaCl (final concentration 3%), at 15 °C and pH 7. The EPS was mainly composed of carbohydrates (35%), followed by proteins and uronic acids (2.5 and 2.77%, respectively) and showed a monosaccharidic composition of glucose: mannose: galactosamine: galactose in the relative molar proportions of 1:0.7:0.5:0.4, as showed by the HPAE-PAD analysis. The detection of specific molecular groups (sulfates and uronic acid content) supported the interesting properties of EPSs, i.e. the emulsifying and cryoprotective action, heavy metal chelation, with interesting implication in bioremediation and biomedical fields. The analysis of the genome allowed to identify a cluster of genes involved in cellulose biosynthesis, and two additional gene clusters putatively involved in EPS biosynthesis. KEY POINTS: • A cold-adapted Pseudoalteromonas strain was investigated for EPS production. • The EPS showed emulsifying, cryoprotective, and heavy metal chelation functions. • Three gene clusters putatively involved in EPS biosynthesis were evidenced by genomic insights. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-12180-x. Text Antarc* Antarctic PubMed Central (PMC) Antarctic Applied Microbiology and Biotechnology 106 21 7173 7185 |
institution |
Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Applied Microbial and Cell Physiology |
spellingShingle |
Applied Microbial and Cell Physiology Rizzo, Carmen Perrin, Elena Poli, Annarita Finore, Ilaria Fani, Renato Lo Giudice, Angelina Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment |
topic_facet |
Applied Microbial and Cell Physiology |
description |
ABSTRACT: A synergistic approach using cultivation methods, chemical, and bioinformatic analyses was applied to explore the potential of Pseudoalteromonas sp. S8-8 in the production of extracellular polymeric substances (EPSs) and the possible physiological traits related to heavy metal and/or antibiotic resistance. The effects of different parameters (carbon source, carbon source concentration, temperature, pH and NaCl supplement) were tested to ensure the optimization of growth conditions for EPS production by the strain S8-8. The highest yield of EPS was obtained during growth in culture medium supplemented with glucose (final concentration 2%) and NaCl (final concentration 3%), at 15 °C and pH 7. The EPS was mainly composed of carbohydrates (35%), followed by proteins and uronic acids (2.5 and 2.77%, respectively) and showed a monosaccharidic composition of glucose: mannose: galactosamine: galactose in the relative molar proportions of 1:0.7:0.5:0.4, as showed by the HPAE-PAD analysis. The detection of specific molecular groups (sulfates and uronic acid content) supported the interesting properties of EPSs, i.e. the emulsifying and cryoprotective action, heavy metal chelation, with interesting implication in bioremediation and biomedical fields. The analysis of the genome allowed to identify a cluster of genes involved in cellulose biosynthesis, and two additional gene clusters putatively involved in EPS biosynthesis. KEY POINTS: • A cold-adapted Pseudoalteromonas strain was investigated for EPS production. • The EPS showed emulsifying, cryoprotective, and heavy metal chelation functions. • Three gene clusters putatively involved in EPS biosynthesis were evidenced by genomic insights. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-12180-x. |
format |
Text |
author |
Rizzo, Carmen Perrin, Elena Poli, Annarita Finore, Ilaria Fani, Renato Lo Giudice, Angelina |
author_facet |
Rizzo, Carmen Perrin, Elena Poli, Annarita Finore, Ilaria Fani, Renato Lo Giudice, Angelina |
author_sort |
Rizzo, Carmen |
title |
Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment |
title_short |
Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment |
title_full |
Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment |
title_fullStr |
Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment |
title_full_unstemmed |
Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment |
title_sort |
characterization of the exopolymer-producing pseudoalteromonas sp. s8-8 from antarctic sediment |
publisher |
Springer Berlin Heidelberg |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592659/ http://www.ncbi.nlm.nih.gov/pubmed/36156161 https://doi.org/10.1007/s00253-022-12180-x |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Appl Microbiol Biotechnol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592659/ http://www.ncbi.nlm.nih.gov/pubmed/36156161 http://dx.doi.org/10.1007/s00253-022-12180-x |
op_rights |
© The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00253-022-12180-x |
container_title |
Applied Microbiology and Biotechnology |
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106 |
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21 |
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7173 |
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7185 |
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