Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models

BACKGROUND: Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important. METHODS: Eight-we...

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Bibliographic Details
Published in:Virology Journal
Main Authors: Tangwangvivat, Ratanaporn, Chaiyawong, Supassama, Nonthabenjawan, Nutthawan, Charoenkul, Kamonpan, Janethanakit, Taveesak, Udom, Kitikhun, Kesdangsakonwut, Sawang, Tantilertcharoen, Rachod, Thontiravong, Aunyaratana, Amonsin, Alongkorn
Format: Text
Language:English
Published: BioMed Central 2022
Subjects:
Research
Canis lupus
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9559841/
https://doi.org/10.1186/s12985-022-01888-x
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Summary:BACKGROUND: Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important. METHODS: Eight-week-old beagle dogs (Canis lupus familaris) (n = 13) were used for the intraspecies transmission model. The dogs were inoculated intranasally with 1 ml of 10(6) EID(50) per ml of canine H3N2 influenza virus (A/canine/Thailand/CU-DC5299/2012) (CIV-H3N2). In addition, 4-week-old guinea pigs (Cavia porcellus) (n = 20) were used for the interspecies transmission model. The guinea pigs were inoculated intranasally with 300 µl of 10(6) EID(50) per ml of CIV-H3N2. RESULTS: For the Thai CIV-H3N2 challenged in the dog model, the incoculated and direct contact dogs developed respiratory signs at 2 dpi. The dogs shed the virus in the respiratory tract at 1 dpi and developed an H3-specific antibody against the virus at 10 dpi. Lung congestion and histopathological changes in the lung were observed. For the Thai CIV-H3N2 challenge in the guinea pig model, the incoculated, direct contact and aerosol-exposed guinea pigs developed fever at 1–2 dpi. The guinea pigs shed virus in the respiratory tract at 2 dpi and developed an H3-specific antibody against the virus at 7 dpi. Mild histopathological changes in the lung were observed. CONCLUSION: The result of this study demonstrated evidence of intraspecies and interspecies transmission of CIV-H3N2 in a mammalian model.

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