Whole-Genome Survey and Microsatellite Marker Detection of Antarctic Crocodile Icefish, Chionobathyscus dewitti
SIMPLE SUMMARY: Crocodile icefish inhabit the deep sea around the Southern Ocean and belong to the family Channichthyidae. The species lacks hemoglobin and has evolved an antifreeze protein, unlike other teleosts. In this study, the whole-genome survey and microsatellite motifs were analyzed, which...
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ftpubmed:oai:pubmedcentral.nih.gov:9558526 2023-05-15T13:47:44+02:00 Whole-Genome Survey and Microsatellite Marker Detection of Antarctic Crocodile Icefish, Chionobathyscus dewitti Kim, Jinmu Lee, Seung-Jae Jo, Euna Choi, Eunkyung Cho, Minjoo Choi, Soyun Kim, Jeong-Hoon Park, Hyun 2022-09-28 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558526/ https://doi.org/10.3390/ani12192598 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558526/ http://dx.doi.org/10.3390/ani12192598 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Animals (Basel) Article Text 2022 ftpubmed https://doi.org/10.3390/ani12192598 2022-10-16T01:02:59Z SIMPLE SUMMARY: Crocodile icefish inhabit the deep sea around the Southern Ocean and belong to the family Channichthyidae. The species lacks hemoglobin and has evolved an antifreeze protein, unlike other teleosts. In this study, the whole-genome survey and microsatellite motifs were analyzed, which provide relevant information on genetic diversity, population genetics, and the genomic study of crocodile icefish. ABSTRACT: The crocodile icefish, Chionobathyscus dewitti, belonging to the family Channichthyidae, is an endemic species of the Southern Ocean. The study of its biological features and genetics is challenging as the fish inhabits the deep sea around Antarctic waters. The icefish, the sole cryopelagic species, shows unique physiological and genetic features, unlike other teleosts. It lacks hemoglobin and has evolved antifreeze proteins. Here, we report the genome sequencing data of crocodile icefish produced using the Illumina Novaseq 6000 platform. The estimated genome size was 0.88 Gb with a K-value of 19, and the unique sequence, heterozygosity, error, and duplication rates were 57.4%, 0.421%, 0.317%, and 0.738%, respectively. A genome assembly of 880.69 Mb, with an N50 scaffold length of 2401 bp, was conducted. We identified 2,252,265 microsatellite motifs from the genome assembly data, and dinucleotide repeats (1,920,127; 85.25%) had the highest rate. We selected 84 primer pairs from the genome survey assembly and randomly selected 30 primer pairs for validation. As a result, 15 primer pairs were validated as microsatellite markers. Text Antarc* Antarctic Icefish Southern Ocean PubMed Central (PMC) Antarctic Southern Ocean Animals 12 19 2598 |
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Article Kim, Jinmu Lee, Seung-Jae Jo, Euna Choi, Eunkyung Cho, Minjoo Choi, Soyun Kim, Jeong-Hoon Park, Hyun Whole-Genome Survey and Microsatellite Marker Detection of Antarctic Crocodile Icefish, Chionobathyscus dewitti |
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Article |
description |
SIMPLE SUMMARY: Crocodile icefish inhabit the deep sea around the Southern Ocean and belong to the family Channichthyidae. The species lacks hemoglobin and has evolved an antifreeze protein, unlike other teleosts. In this study, the whole-genome survey and microsatellite motifs were analyzed, which provide relevant information on genetic diversity, population genetics, and the genomic study of crocodile icefish. ABSTRACT: The crocodile icefish, Chionobathyscus dewitti, belonging to the family Channichthyidae, is an endemic species of the Southern Ocean. The study of its biological features and genetics is challenging as the fish inhabits the deep sea around Antarctic waters. The icefish, the sole cryopelagic species, shows unique physiological and genetic features, unlike other teleosts. It lacks hemoglobin and has evolved antifreeze proteins. Here, we report the genome sequencing data of crocodile icefish produced using the Illumina Novaseq 6000 platform. The estimated genome size was 0.88 Gb with a K-value of 19, and the unique sequence, heterozygosity, error, and duplication rates were 57.4%, 0.421%, 0.317%, and 0.738%, respectively. A genome assembly of 880.69 Mb, with an N50 scaffold length of 2401 bp, was conducted. We identified 2,252,265 microsatellite motifs from the genome assembly data, and dinucleotide repeats (1,920,127; 85.25%) had the highest rate. We selected 84 primer pairs from the genome survey assembly and randomly selected 30 primer pairs for validation. As a result, 15 primer pairs were validated as microsatellite markers. |
format |
Text |
author |
Kim, Jinmu Lee, Seung-Jae Jo, Euna Choi, Eunkyung Cho, Minjoo Choi, Soyun Kim, Jeong-Hoon Park, Hyun |
author_facet |
Kim, Jinmu Lee, Seung-Jae Jo, Euna Choi, Eunkyung Cho, Minjoo Choi, Soyun Kim, Jeong-Hoon Park, Hyun |
author_sort |
Kim, Jinmu |
title |
Whole-Genome Survey and Microsatellite Marker Detection of Antarctic Crocodile Icefish, Chionobathyscus dewitti |
title_short |
Whole-Genome Survey and Microsatellite Marker Detection of Antarctic Crocodile Icefish, Chionobathyscus dewitti |
title_full |
Whole-Genome Survey and Microsatellite Marker Detection of Antarctic Crocodile Icefish, Chionobathyscus dewitti |
title_fullStr |
Whole-Genome Survey and Microsatellite Marker Detection of Antarctic Crocodile Icefish, Chionobathyscus dewitti |
title_full_unstemmed |
Whole-Genome Survey and Microsatellite Marker Detection of Antarctic Crocodile Icefish, Chionobathyscus dewitti |
title_sort |
whole-genome survey and microsatellite marker detection of antarctic crocodile icefish, chionobathyscus dewitti |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558526/ https://doi.org/10.3390/ani12192598 |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Icefish Southern Ocean |
genre_facet |
Antarc* Antarctic Icefish Southern Ocean |
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Animals (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558526/ http://dx.doi.org/10.3390/ani12192598 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/ani12192598 |
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Animals |
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2598 |
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1766247778731687936 |