Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern
Adventitious root (AR) formation is the basis of vegetative propagation in rose, be it via stem cuttings or via stenting. During this process, wounding plays a pivotal role since cell reprogramming takes place at the tissue adjacent to the wound. We investigated the effects of wounding on AR formati...
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ftpubmed:oai:pubmedcentral.nih.gov:9557736 2023-05-15T15:41:56+02:00 Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern Morales-Orellana, Raul Javier Winkelmann, Traud Bettin, Andreas Rath, Thomas 2022-09-29 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557736/ https://doi.org/10.3389/fpls.2022.1009085 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557736/ http://dx.doi.org/10.3389/fpls.2022.1009085 Copyright © 2022 Morales-Orellana, Winkelmann, Bettin and Rath https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Plant Sci Plant Science Text 2022 ftpubmed https://doi.org/10.3389/fpls.2022.1009085 2022-10-16T01:01:31Z Adventitious root (AR) formation is the basis of vegetative propagation in rose, be it via stem cuttings or via stenting. During this process, wounding plays a pivotal role since cell reprogramming takes place at the tissue adjacent to the wound. We investigated the effects of wounding on AR formation on leafy single-node stem cuttings of the rose rootstock R. canina ‘Pfänder’ (codes R02-3 and R02-6) and the cut rose cultivar Rosa ‘Tan09283’ (Registration name ‘Beluga’). Laser wounding treatments were based on the assisted removal of tissue layers located in the bark. The positioning of wounding was studied based on two marking directions: along the cutting base (strip pattern) and around the cutting base (ring pattern). Additionally, the effects of external supply of indole-butyric acid (IBA 1 mg L(-1)) on rooting were analyzed. Results showed that in order to remove specific tissue layers, the calculation of the laser energy density (J cm(-2)) in terms of cutting diameter was necessary. Interestingly, the application of energy densities from 2.5 J cm(-2) up to approximately 8.5 J cm(-2) were sufficient to expose the tissue layers of epidermis up to regions of phloem. Regarding AR formation for R. canina ‘Pfänder’, characterized by a low rooting response, an increase in the rooting percentage was registered when the laser treatment eliminated the tissue up to phloem proximities. Analysis of the nodal position showed that bud location was a preferential place for AR formation independently of wounding treatment. In case of Rosa ‘Tan09283’, laser treatments did not reduce its high rooting capacity, but an apparent reduction in rooting quality due to an investment in tissue healing was observed when wounding reached deeper layers such as parenchyma and sclerenchyma. Results also showed a strong AR formation directly from wounded regions in case of Rosa ‘Tan09283’ specifically when the wound was located below the axillary bud. In conclusion, wounding by assisted-elimination of layers by laser can induce positive ... Text Beluga Beluga* PubMed Central (PMC) Frontiers in Plant Science 13 |
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Plant Science |
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Plant Science Morales-Orellana, Raul Javier Winkelmann, Traud Bettin, Andreas Rath, Thomas Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern |
topic_facet |
Plant Science |
description |
Adventitious root (AR) formation is the basis of vegetative propagation in rose, be it via stem cuttings or via stenting. During this process, wounding plays a pivotal role since cell reprogramming takes place at the tissue adjacent to the wound. We investigated the effects of wounding on AR formation on leafy single-node stem cuttings of the rose rootstock R. canina ‘Pfänder’ (codes R02-3 and R02-6) and the cut rose cultivar Rosa ‘Tan09283’ (Registration name ‘Beluga’). Laser wounding treatments were based on the assisted removal of tissue layers located in the bark. The positioning of wounding was studied based on two marking directions: along the cutting base (strip pattern) and around the cutting base (ring pattern). Additionally, the effects of external supply of indole-butyric acid (IBA 1 mg L(-1)) on rooting were analyzed. Results showed that in order to remove specific tissue layers, the calculation of the laser energy density (J cm(-2)) in terms of cutting diameter was necessary. Interestingly, the application of energy densities from 2.5 J cm(-2) up to approximately 8.5 J cm(-2) were sufficient to expose the tissue layers of epidermis up to regions of phloem. Regarding AR formation for R. canina ‘Pfänder’, characterized by a low rooting response, an increase in the rooting percentage was registered when the laser treatment eliminated the tissue up to phloem proximities. Analysis of the nodal position showed that bud location was a preferential place for AR formation independently of wounding treatment. In case of Rosa ‘Tan09283’, laser treatments did not reduce its high rooting capacity, but an apparent reduction in rooting quality due to an investment in tissue healing was observed when wounding reached deeper layers such as parenchyma and sclerenchyma. Results also showed a strong AR formation directly from wounded regions in case of Rosa ‘Tan09283’ specifically when the wound was located below the axillary bud. In conclusion, wounding by assisted-elimination of layers by laser can induce positive ... |
format |
Text |
author |
Morales-Orellana, Raul Javier Winkelmann, Traud Bettin, Andreas Rath, Thomas |
author_facet |
Morales-Orellana, Raul Javier Winkelmann, Traud Bettin, Andreas Rath, Thomas |
author_sort |
Morales-Orellana, Raul Javier |
title |
Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern |
title_short |
Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern |
title_full |
Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern |
title_fullStr |
Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern |
title_full_unstemmed |
Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern |
title_sort |
stimulation of adventitious root formation by laser wounding in rose cuttings: a matter of energy and pattern |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557736/ https://doi.org/10.3389/fpls.2022.1009085 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Front Plant Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557736/ http://dx.doi.org/10.3389/fpls.2022.1009085 |
op_rights |
Copyright © 2022 Morales-Orellana, Winkelmann, Bettin and Rath https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2022.1009085 |
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Frontiers in Plant Science |
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13 |
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1766374819843014656 |