Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport
Water uptake by roots is a key adaptation of plants to aerial life. Water uptake depends on root system architecture (RSA) and tissue hydraulic properties that, together, shape the root hydraulic architecture. This work investigates how the interplay between conductivities along radial (e.g. aquapor...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516777/ http://www.ncbi.nlm.nih.gov/pubmed/35708646 https://doi.org/10.1093/plphys/kiac281 |
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ftpubmed:oai:pubmedcentral.nih.gov:9516777 2023-05-15T16:07:28+02:00 Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport Boursiac, Yann Pradal, Christophe Bauget, Fabrice Lucas, Mikaël Delivorias, Stathis Godin, Christophe Maurel, Christophe 2022-06-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516777/ http://www.ncbi.nlm.nih.gov/pubmed/35708646 https://doi.org/10.1093/plphys/kiac281 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516777/ http://www.ncbi.nlm.nih.gov/pubmed/35708646 http://dx.doi.org/10.1093/plphys/kiac281 © The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com CC-BY-NC-ND Plant Physiol Regular Issue Content Text 2022 ftpubmed https://doi.org/10.1093/plphys/kiac281 2022-10-02T00:58:11Z Water uptake by roots is a key adaptation of plants to aerial life. Water uptake depends on root system architecture (RSA) and tissue hydraulic properties that, together, shape the root hydraulic architecture. This work investigates how the interplay between conductivities along radial (e.g. aquaporins) and axial (e.g. xylem vessels) pathways determines the water transport properties of highly branched RSAs as found in adult Arabidopsis (Arabidopsis thaliana) plants. A hydraulic model named HydroRoot was developed, based on multi-scale tree graph representations of RSAs. Root water flow was measured by the pressure chamber technique after successive cuts of a same root system from the tip toward the base. HydroRoot model inversion in corresponding RSAs allowed us to concomitantly determine radial and axial conductivities, providing evidence that the latter is often overestimated by classical evaluation based on the Hagen–Poiseuille law. Organizing principles of Arabidopsis primary and lateral root growth and branching were determined and used to apply the HydroRoot model to an extended set of simulated RSAs. Sensitivity analyses revealed that water transport can be co-limited by radial and axial conductances throughout the whole RSA. The number of roots that can be sectioned (intercepted) at a given distance from the base was defined as an accessible and informative indicator of RSA. The overall set of experimental and theoretical procedures was applied to plants mutated in ESKIMO1 and previously shown to have xylem collapse. This approach will be instrumental to dissect the root water transport phenotype of plants with intricate alterations in root growth or transport functions. Text eskimo* PubMed Central (PMC) Hagen ENVELOPE(6.545,6.545,62.545,62.545) Plant Physiology 190 2 1289 1306 |
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Regular Issue Content Boursiac, Yann Pradal, Christophe Bauget, Fabrice Lucas, Mikaël Delivorias, Stathis Godin, Christophe Maurel, Christophe Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
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Water uptake by roots is a key adaptation of plants to aerial life. Water uptake depends on root system architecture (RSA) and tissue hydraulic properties that, together, shape the root hydraulic architecture. This work investigates how the interplay between conductivities along radial (e.g. aquaporins) and axial (e.g. xylem vessels) pathways determines the water transport properties of highly branched RSAs as found in adult Arabidopsis (Arabidopsis thaliana) plants. A hydraulic model named HydroRoot was developed, based on multi-scale tree graph representations of RSAs. Root water flow was measured by the pressure chamber technique after successive cuts of a same root system from the tip toward the base. HydroRoot model inversion in corresponding RSAs allowed us to concomitantly determine radial and axial conductivities, providing evidence that the latter is often overestimated by classical evaluation based on the Hagen–Poiseuille law. Organizing principles of Arabidopsis primary and lateral root growth and branching were determined and used to apply the HydroRoot model to an extended set of simulated RSAs. Sensitivity analyses revealed that water transport can be co-limited by radial and axial conductances throughout the whole RSA. The number of roots that can be sectioned (intercepted) at a given distance from the base was defined as an accessible and informative indicator of RSA. The overall set of experimental and theoretical procedures was applied to plants mutated in ESKIMO1 and previously shown to have xylem collapse. This approach will be instrumental to dissect the root water transport phenotype of plants with intricate alterations in root growth or transport functions. |
format |
Text |
author |
Boursiac, Yann Pradal, Christophe Bauget, Fabrice Lucas, Mikaël Delivorias, Stathis Godin, Christophe Maurel, Christophe |
author_facet |
Boursiac, Yann Pradal, Christophe Bauget, Fabrice Lucas, Mikaël Delivorias, Stathis Godin, Christophe Maurel, Christophe |
author_sort |
Boursiac, Yann |
title |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_short |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_full |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_fullStr |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_full_unstemmed |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_sort |
phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
publisher |
Oxford University Press |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516777/ http://www.ncbi.nlm.nih.gov/pubmed/35708646 https://doi.org/10.1093/plphys/kiac281 |
long_lat |
ENVELOPE(6.545,6.545,62.545,62.545) |
geographic |
Hagen |
geographic_facet |
Hagen |
genre |
eskimo* |
genre_facet |
eskimo* |
op_source |
Plant Physiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516777/ http://www.ncbi.nlm.nih.gov/pubmed/35708646 http://dx.doi.org/10.1093/plphys/kiac281 |
op_rights |
© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1093/plphys/kiac281 |
container_title |
Plant Physiology |
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190 |
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2 |
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1289 |
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1306 |
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