“Guess Who’s Coming to Dinner”: Molecular Tools to Reconstruct multilocus Genetic Profiles from Wild Canid Consumption Remains
SIMPLE SUMMARY: Wolves and European wildcats are two iconic predator species that can live in overlapping ecological contexts and also share their habitats with their domestic free-ranging relatives, increasing the risk of anthropogenic hybridisation and its possible deleterious consequences. By app...
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ftpubmed:oai:pubmedcentral.nih.gov:9495216 2023-05-15T15:50:59+02:00 “Guess Who’s Coming to Dinner”: Molecular Tools to Reconstruct multilocus Genetic Profiles from Wild Canid Consumption Remains Velli, Edoardo Mattucci, Federica Lazzeri, Lorenzo Fabbri, Elena Pacini, Giada Belardi, Irene Mucci, Nadia Caniglia, Romolo 2022-09-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495216/ https://doi.org/10.3390/ani12182428 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495216/ http://dx.doi.org/10.3390/ani12182428 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Animals (Basel) Communication Text 2022 ftpubmed https://doi.org/10.3390/ani12182428 2022-09-25T01:10:39Z SIMPLE SUMMARY: Wolves and European wildcats are two iconic predator species that can live in overlapping ecological contexts and also share their habitats with their domestic free-ranging relatives, increasing the risk of anthropogenic hybridisation and its possible deleterious consequences. By applying a multidisciplinary approach, we morphologically and molecularly analysed the cat remains found in a canid faecal sample collected in a forested area of central Italy. Individual multilocus genotypes of both predator and prey were identified turning out to be, respectively, a wolf showing traces of dog ancestry at autosomal microsatellite loci and a domestic cat. ABSTRACT: Non-invasive genetic sampling is a practical tool to monitor pivotal ecological parameters and population dynamic patterns of endangered species. It can be particularly suitable when applied to elusive carnivores such as the Apennine wolf (Canis lupus italicus) and the European wildcat (Felis silvestris silvestris), which can live in overlapping ecological contexts and sometimes share their habitats with their domestic free-ranging relatives, increasing the risk of anthropogenic hybridisation. In this case study, we exploited all the ecological and genetic information contained in a single biological canid faecal sample, collected in a forested area of central Italy, to detect any sign of trophic interactions between wolves and European wildcats or their domestic counterparts. Firstly, the faecal finding was morphologically examined, showing the presence of felid hair and claw fragment remains. Subsequently, total genomic DNA contained in the hair and claw samples was extracted and genotyped, through a multiple-tube approach, at canid and felid diagnostic panels of microsatellite loci. Finally, the obtained individual multilocus genotypes were analysed with reference wild and domestic canid and felid populations to assess their correct taxonomic status using Bayesian clustering procedures. Assignment analyses classified the genotype obtained ... Text Canis lupus PubMed Central (PMC) Animals 12 18 2428 |
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Communication Velli, Edoardo Mattucci, Federica Lazzeri, Lorenzo Fabbri, Elena Pacini, Giada Belardi, Irene Mucci, Nadia Caniglia, Romolo “Guess Who’s Coming to Dinner”: Molecular Tools to Reconstruct multilocus Genetic Profiles from Wild Canid Consumption Remains |
topic_facet |
Communication |
description |
SIMPLE SUMMARY: Wolves and European wildcats are two iconic predator species that can live in overlapping ecological contexts and also share their habitats with their domestic free-ranging relatives, increasing the risk of anthropogenic hybridisation and its possible deleterious consequences. By applying a multidisciplinary approach, we morphologically and molecularly analysed the cat remains found in a canid faecal sample collected in a forested area of central Italy. Individual multilocus genotypes of both predator and prey were identified turning out to be, respectively, a wolf showing traces of dog ancestry at autosomal microsatellite loci and a domestic cat. ABSTRACT: Non-invasive genetic sampling is a practical tool to monitor pivotal ecological parameters and population dynamic patterns of endangered species. It can be particularly suitable when applied to elusive carnivores such as the Apennine wolf (Canis lupus italicus) and the European wildcat (Felis silvestris silvestris), which can live in overlapping ecological contexts and sometimes share their habitats with their domestic free-ranging relatives, increasing the risk of anthropogenic hybridisation. In this case study, we exploited all the ecological and genetic information contained in a single biological canid faecal sample, collected in a forested area of central Italy, to detect any sign of trophic interactions between wolves and European wildcats or their domestic counterparts. Firstly, the faecal finding was morphologically examined, showing the presence of felid hair and claw fragment remains. Subsequently, total genomic DNA contained in the hair and claw samples was extracted and genotyped, through a multiple-tube approach, at canid and felid diagnostic panels of microsatellite loci. Finally, the obtained individual multilocus genotypes were analysed with reference wild and domestic canid and felid populations to assess their correct taxonomic status using Bayesian clustering procedures. Assignment analyses classified the genotype obtained ... |
format |
Text |
author |
Velli, Edoardo Mattucci, Federica Lazzeri, Lorenzo Fabbri, Elena Pacini, Giada Belardi, Irene Mucci, Nadia Caniglia, Romolo |
author_facet |
Velli, Edoardo Mattucci, Federica Lazzeri, Lorenzo Fabbri, Elena Pacini, Giada Belardi, Irene Mucci, Nadia Caniglia, Romolo |
author_sort |
Velli, Edoardo |
title |
“Guess Who’s Coming to Dinner”: Molecular Tools to Reconstruct multilocus Genetic Profiles from Wild Canid Consumption Remains |
title_short |
“Guess Who’s Coming to Dinner”: Molecular Tools to Reconstruct multilocus Genetic Profiles from Wild Canid Consumption Remains |
title_full |
“Guess Who’s Coming to Dinner”: Molecular Tools to Reconstruct multilocus Genetic Profiles from Wild Canid Consumption Remains |
title_fullStr |
“Guess Who’s Coming to Dinner”: Molecular Tools to Reconstruct multilocus Genetic Profiles from Wild Canid Consumption Remains |
title_full_unstemmed |
“Guess Who’s Coming to Dinner”: Molecular Tools to Reconstruct multilocus Genetic Profiles from Wild Canid Consumption Remains |
title_sort |
“guess who’s coming to dinner”: molecular tools to reconstruct multilocus genetic profiles from wild canid consumption remains |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495216/ https://doi.org/10.3390/ani12182428 |
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Canis lupus |
genre_facet |
Canis lupus |
op_source |
Animals (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9495216/ http://dx.doi.org/10.3390/ani12182428 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/ani12182428 |
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Animals |
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12 |
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18 |
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2428 |
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1766386022190415872 |