Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins
The global decline of natural oyster populations emphasizes the need to improve our understanding of their biology. Understanding the role of chemical cues from conspecifics on how oysters occupy appropriate substrata is crucial to learning about their evolution, population dynamics, and chemical co...
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ftpubmed:oai:pubmedcentral.nih.gov:9456362 2023-05-15T15:58:07+02:00 Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins Sedanza, Mary Grace Yoshida, Asami Kim, Hee-Jin Yamaguchi, Kenichi Osatomi, Kiyoshi Satuito, Cyril Glenn 2022-08-29 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456362/ https://doi.org/10.3390/ijms23179816 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456362/ http://dx.doi.org/10.3390/ijms23179816 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Int J Mol Sci Article Text 2022 ftpubmed https://doi.org/10.3390/ijms23179816 2022-09-11T01:10:10Z The global decline of natural oyster populations emphasizes the need to improve our understanding of their biology. Understanding the role of chemical cues from conspecifics on how oysters occupy appropriate substrata is crucial to learning about their evolution, population dynamics, and chemical communication. Here, a novel role of a macromolecular assembly of shell matrix proteins which act as Crassostrea gigas Settlement Pheromone Protein Components in adult shells is demonstrated as the biological cue responsible for gregarious settlement on conspecifics. A bioassay-guided fractionation approach aided by biochemical and molecular analyses reveals that Gigasin-6 isoform X1 and/or X2 isolated from adult shells is the major inducing cue for larval settlement and may also play a role in postlarva–larva settlement interactions. Other isolated Stains-all-stainable acidic proteins may function as a co-factor and a scaffold/structural framework for other matrix proteins to anchor within this assembly and provide protection. Notably, conspecific cue-mediated larval settlement induction in C. gigas presents a complex system that requires an interplay of different glycans, disulfide bonds, amino acid groups, and phosphorylation crosstalk for recognition. These results may find application in the development of oyster aquacultures which could help recover declining marine species and as targets of anti-fouling agents. Text Crassostrea gigas PubMed Central (PMC) International Journal of Molecular Sciences 23 17 9816 |
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Article Sedanza, Mary Grace Yoshida, Asami Kim, Hee-Jin Yamaguchi, Kenichi Osatomi, Kiyoshi Satuito, Cyril Glenn Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
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Article |
description |
The global decline of natural oyster populations emphasizes the need to improve our understanding of their biology. Understanding the role of chemical cues from conspecifics on how oysters occupy appropriate substrata is crucial to learning about their evolution, population dynamics, and chemical communication. Here, a novel role of a macromolecular assembly of shell matrix proteins which act as Crassostrea gigas Settlement Pheromone Protein Components in adult shells is demonstrated as the biological cue responsible for gregarious settlement on conspecifics. A bioassay-guided fractionation approach aided by biochemical and molecular analyses reveals that Gigasin-6 isoform X1 and/or X2 isolated from adult shells is the major inducing cue for larval settlement and may also play a role in postlarva–larva settlement interactions. Other isolated Stains-all-stainable acidic proteins may function as a co-factor and a scaffold/structural framework for other matrix proteins to anchor within this assembly and provide protection. Notably, conspecific cue-mediated larval settlement induction in C. gigas presents a complex system that requires an interplay of different glycans, disulfide bonds, amino acid groups, and phosphorylation crosstalk for recognition. These results may find application in the development of oyster aquacultures which could help recover declining marine species and as targets of anti-fouling agents. |
format |
Text |
author |
Sedanza, Mary Grace Yoshida, Asami Kim, Hee-Jin Yamaguchi, Kenichi Osatomi, Kiyoshi Satuito, Cyril Glenn |
author_facet |
Sedanza, Mary Grace Yoshida, Asami Kim, Hee-Jin Yamaguchi, Kenichi Osatomi, Kiyoshi Satuito, Cyril Glenn |
author_sort |
Sedanza, Mary Grace |
title |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_short |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_full |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_fullStr |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_full_unstemmed |
Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
title_sort |
identification and characterization of the larval settlement pheromone protein components in adult shells of crassostrea gigas: a novel function of shell matrix proteins |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456362/ https://doi.org/10.3390/ijms23179816 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Int J Mol Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456362/ http://dx.doi.org/10.3390/ijms23179816 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/ijms23179816 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
17 |
container_start_page |
9816 |
_version_ |
1766393844542210048 |