A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats
In this study, a novel peptide, AEYLCEAC with high angiotensin-I-converting enzyme inhibitory (ACEI) activity was screened from oyster (Crassostrea gigas) hydrolysates, which was obtained from simulated gastro-intestinal digestion. Candidate peptides were confirmed to have a higher binding to angiot...
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ftpubmed:oai:pubmedcentral.nih.gov:9445672 2023-05-15T15:58:35+02:00 A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats Chen, Hui Chen, Yu Zheng, Huizhen Xiang, Xingwei Xu, Lu 2022-08-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445672/ https://doi.org/10.3389/fnut.2022.981163 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445672/ http://dx.doi.org/10.3389/fnut.2022.981163 Copyright © 2022 Chen, Chen, Zheng, Xiang and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Nutr Nutrition Text 2022 ftpubmed https://doi.org/10.3389/fnut.2022.981163 2022-09-11T00:49:39Z In this study, a novel peptide, AEYLCEAC with high angiotensin-I-converting enzyme inhibitory (ACEI) activity was screened from oyster (Crassostrea gigas) hydrolysates, which was obtained from simulated gastro-intestinal digestion. Candidate peptides were confirmed to have a higher binding to angiotensin-I-converting enzyme (ACE) than the positive drug phosphoinic tripeptide calculated by Discovery Studio, and AEYLCEAC showed the highest ACE inhibition rate in vitro with a IC(50) of 4.287 mM. Lineweaver-Burk plots confirmed that the peptidic inhibitory type of ACE is competitive. The molecular docking showed that ACEI activity of the AEYLCEAC was mainly due to the hydrogen bonding interactions with the active pockets (S1 and S2) of ACE. In vivo, AEYLCEAC effectively reduced diastolic blood pressure (DBP) and Systolic blood pressure (SBP) in hypertensive rats. These results indicate that AEYLCEAC might act as a helpful ingredient in functional foods or pharmaceuticals for the prevention and treatment of hypertension. Text Crassostrea gigas PubMed Central (PMC) Frontiers in Nutrition 9 |
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Nutrition |
spellingShingle |
Nutrition Chen, Hui Chen, Yu Zheng, Huizhen Xiang, Xingwei Xu, Lu A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats |
topic_facet |
Nutrition |
description |
In this study, a novel peptide, AEYLCEAC with high angiotensin-I-converting enzyme inhibitory (ACEI) activity was screened from oyster (Crassostrea gigas) hydrolysates, which was obtained from simulated gastro-intestinal digestion. Candidate peptides were confirmed to have a higher binding to angiotensin-I-converting enzyme (ACE) than the positive drug phosphoinic tripeptide calculated by Discovery Studio, and AEYLCEAC showed the highest ACE inhibition rate in vitro with a IC(50) of 4.287 mM. Lineweaver-Burk plots confirmed that the peptidic inhibitory type of ACE is competitive. The molecular docking showed that ACEI activity of the AEYLCEAC was mainly due to the hydrogen bonding interactions with the active pockets (S1 and S2) of ACE. In vivo, AEYLCEAC effectively reduced diastolic blood pressure (DBP) and Systolic blood pressure (SBP) in hypertensive rats. These results indicate that AEYLCEAC might act as a helpful ingredient in functional foods or pharmaceuticals for the prevention and treatment of hypertension. |
format |
Text |
author |
Chen, Hui Chen, Yu Zheng, Huizhen Xiang, Xingwei Xu, Lu |
author_facet |
Chen, Hui Chen, Yu Zheng, Huizhen Xiang, Xingwei Xu, Lu |
author_sort |
Chen, Hui |
title |
A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats |
title_short |
A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats |
title_full |
A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats |
title_fullStr |
A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats |
title_full_unstemmed |
A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats |
title_sort |
novel angiotensin-i-converting enzyme inhibitory peptide from oyster: simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445672/ https://doi.org/10.3389/fnut.2022.981163 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Front Nutr |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445672/ http://dx.doi.org/10.3389/fnut.2022.981163 |
op_rights |
Copyright © 2022 Chen, Chen, Zheng, Xiang and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fnut.2022.981163 |
container_title |
Frontiers in Nutrition |
container_volume |
9 |
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1766394351873687552 |