Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State
[Image: see text] The decades-long ultrafast examination of nearly a dozen microbial retinal proteins, ion pumps, and sensory photoreceptors has not identified structure–function indicators which predict photoisomerization dynamics, whether it will be sub-picosecond and ballistic or drawn out with c...
Published in: | The Journal of Physical Chemistry Letters |
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American Chemical Society
2022
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442786/ http://www.ncbi.nlm.nih.gov/pubmed/36000820 https://doi.org/10.1021/acs.jpclett.2c01974 |
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ftpubmed:oai:pubmedcentral.nih.gov:9442786 2023-05-15T13:54:00+02:00 Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State Malakar, Partha Das, Ishita Bhattacharya, Sudeshna Harris, Andrew Sheves, Mordechai Brown, Leonid S. Ruhman, Sanford 2022-08-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442786/ http://www.ncbi.nlm.nih.gov/pubmed/36000820 https://doi.org/10.1021/acs.jpclett.2c01974 en eng American Chemical Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442786/ http://www.ncbi.nlm.nih.gov/pubmed/36000820 http://dx.doi.org/10.1021/acs.jpclett.2c01974 © 2022 American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). CC-BY J Phys Chem Lett Text 2022 ftpubmed https://doi.org/10.1021/acs.jpclett.2c01974 2022-09-11T00:43:29Z [Image: see text] The decades-long ultrafast examination of nearly a dozen microbial retinal proteins, ion pumps, and sensory photoreceptors has not identified structure–function indicators which predict photoisomerization dynamics, whether it will be sub-picosecond and ballistic or drawn out with complex curve-crossing kinetics. Herein, we report the emergence of such an indicator. Using pH control over retinal isomer ratios, photoinduced transient absorption is recorded in an inward proton pumping Antarctic microbial rhodopsin (AntR) for 13-cis and all-trans retinal resting states. The all-trans fluorescent state decays with 1 ps exponential kinetics. In contrast, in 13-cis it decays within ∼300 fs accompanied by continuous spectral evolution, indicating ballistic internal conversion. The coherent wave packet nature of 13-cis isomerization in AntR matches published results for bacteriorhodopsin (BR) and Anabaena sensory rhodopsin (ASR), which also accommodate both all-trans and 13-cis retinal resting states, marking the emergence of a first structure–photodynamics indicator which holds for all three tested pigments. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Journal of Physical Chemistry Letters 13 34 8134 8140 |
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English |
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[Image: see text] The decades-long ultrafast examination of nearly a dozen microbial retinal proteins, ion pumps, and sensory photoreceptors has not identified structure–function indicators which predict photoisomerization dynamics, whether it will be sub-picosecond and ballistic or drawn out with complex curve-crossing kinetics. Herein, we report the emergence of such an indicator. Using pH control over retinal isomer ratios, photoinduced transient absorption is recorded in an inward proton pumping Antarctic microbial rhodopsin (AntR) for 13-cis and all-trans retinal resting states. The all-trans fluorescent state decays with 1 ps exponential kinetics. In contrast, in 13-cis it decays within ∼300 fs accompanied by continuous spectral evolution, indicating ballistic internal conversion. The coherent wave packet nature of 13-cis isomerization in AntR matches published results for bacteriorhodopsin (BR) and Anabaena sensory rhodopsin (ASR), which also accommodate both all-trans and 13-cis retinal resting states, marking the emergence of a first structure–photodynamics indicator which holds for all three tested pigments. |
format |
Text |
author |
Malakar, Partha Das, Ishita Bhattacharya, Sudeshna Harris, Andrew Sheves, Mordechai Brown, Leonid S. Ruhman, Sanford |
spellingShingle |
Malakar, Partha Das, Ishita Bhattacharya, Sudeshna Harris, Andrew Sheves, Mordechai Brown, Leonid S. Ruhman, Sanford Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State |
author_facet |
Malakar, Partha Das, Ishita Bhattacharya, Sudeshna Harris, Andrew Sheves, Mordechai Brown, Leonid S. Ruhman, Sanford |
author_sort |
Malakar, Partha |
title |
Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State |
title_short |
Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State |
title_full |
Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State |
title_fullStr |
Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State |
title_full_unstemmed |
Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-cis Resting State |
title_sort |
bidirectional photochemistry of antarctic microbial rhodopsin: emerging trend of ballistic photoisomerization from the 13-cis resting state |
publisher |
American Chemical Society |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442786/ http://www.ncbi.nlm.nih.gov/pubmed/36000820 https://doi.org/10.1021/acs.jpclett.2c01974 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
J Phys Chem Lett |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442786/ http://www.ncbi.nlm.nih.gov/pubmed/36000820 http://dx.doi.org/10.1021/acs.jpclett.2c01974 |
op_rights |
© 2022 American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1021/acs.jpclett.2c01974 |
container_title |
The Journal of Physical Chemistry Letters |
container_volume |
13 |
container_issue |
34 |
container_start_page |
8134 |
op_container_end_page |
8140 |
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1766259495441268736 |