Gut Microbiota and Transcriptomics Reveal the Effect of Human Norovirus Bioaccumulation on Oysters (Crassostrea gigas)
Human norovirus (HuNoV) is a major foodborne pathogen that causes acute viral gastroenteritis, and oysters are one of the main carriers of HuNoV transmission. While progress has been made toward understanding the pattern of oyster-bioaccumulated HuNoV, the response of oysters to HuNoV bioaccumulatio...
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ftpubmed:oai:pubmedcentral.nih.gov:9431538 2023-05-15T15:58:52+02:00 Gut Microbiota and Transcriptomics Reveal the Effect of Human Norovirus Bioaccumulation on Oysters (Crassostrea gigas) Yang, Min Tong, Lihui Wang, Shanshan Liu, Nan Zhao, Feng Sun, Yong Sun, Guohui Zhou, Deqing 2022-07-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9431538/ http://www.ncbi.nlm.nih.gov/pubmed/35867424 https://doi.org/10.1128/spectrum.00161-22 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9431538/ http://www.ncbi.nlm.nih.gov/pubmed/35867424 http://dx.doi.org/10.1128/spectrum.00161-22 Copyright © 2022 Yang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . CC-BY Microbiol Spectr Research Article Text 2022 ftpubmed https://doi.org/10.1128/spectrum.00161-22 2022-09-04T01:16:20Z Human norovirus (HuNoV) is a major foodborne pathogen that causes acute viral gastroenteritis, and oysters are one of the main carriers of HuNoV transmission. While progress has been made toward understanding the pattern of oyster-bioaccumulated HuNoV, the response of oysters to HuNoV bioaccumulation, including changes in gene expression and gut microbiota, is unclear. In this study, histo-blood group antigen (HBGA)-like molecule expression and gene regulation features and the HuNoV-microbiome interactions of oysters during HuNoV bioaccumulation were characterized. With the prolongation of bioaccumulation time, the HuNoV content and expression of type A HBGA-like molecules in oysters increased and stabilized. HuNoV also altered the expression of immunity- and glycosphingolipid biosynthesis-related genes. Prolonged bioaccumulation of HuNoV can reduce the abundance and change the composition of the oyster gut microbiota. In particular, with the extension of bioaccumulation time, the abundance of Blautia, Agathobacter, Faecalibacterium, Terrisporobacter, Bifidobacterium, Lactobacillus, and Ruminococcus decreased, while the abundance of Vibrio and Alphaproteobacteria increased. This study provides potential candidates for identifying functional genes involved in the bioaccumulation of HuNoV in oysters. More importantly, it provides the first description of the changes in gut microbiota during HuNoV bioaccumulation in oysters. IMPORTANCE The role of the oyster gut microbiota in HuNoV bioaccumulation is poorly understood. This study revealed, for the first time, the changes in gut microbiota and gene expression of oysters with HuNoV bioaccumulation. This study enriches the understanding of the impact of HuNoV bioaccumulation on oysters and provides a new direction for the study of the molecular mechanism of HuNoV bioaccumulation in oysters. Text Crassostrea gigas PubMed Central (PMC) Microbiology Spectrum 10 4 |
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Research Article Yang, Min Tong, Lihui Wang, Shanshan Liu, Nan Zhao, Feng Sun, Yong Sun, Guohui Zhou, Deqing Gut Microbiota and Transcriptomics Reveal the Effect of Human Norovirus Bioaccumulation on Oysters (Crassostrea gigas) |
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Research Article |
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Human norovirus (HuNoV) is a major foodborne pathogen that causes acute viral gastroenteritis, and oysters are one of the main carriers of HuNoV transmission. While progress has been made toward understanding the pattern of oyster-bioaccumulated HuNoV, the response of oysters to HuNoV bioaccumulation, including changes in gene expression and gut microbiota, is unclear. In this study, histo-blood group antigen (HBGA)-like molecule expression and gene regulation features and the HuNoV-microbiome interactions of oysters during HuNoV bioaccumulation were characterized. With the prolongation of bioaccumulation time, the HuNoV content and expression of type A HBGA-like molecules in oysters increased and stabilized. HuNoV also altered the expression of immunity- and glycosphingolipid biosynthesis-related genes. Prolonged bioaccumulation of HuNoV can reduce the abundance and change the composition of the oyster gut microbiota. In particular, with the extension of bioaccumulation time, the abundance of Blautia, Agathobacter, Faecalibacterium, Terrisporobacter, Bifidobacterium, Lactobacillus, and Ruminococcus decreased, while the abundance of Vibrio and Alphaproteobacteria increased. This study provides potential candidates for identifying functional genes involved in the bioaccumulation of HuNoV in oysters. More importantly, it provides the first description of the changes in gut microbiota during HuNoV bioaccumulation in oysters. IMPORTANCE The role of the oyster gut microbiota in HuNoV bioaccumulation is poorly understood. This study revealed, for the first time, the changes in gut microbiota and gene expression of oysters with HuNoV bioaccumulation. This study enriches the understanding of the impact of HuNoV bioaccumulation on oysters and provides a new direction for the study of the molecular mechanism of HuNoV bioaccumulation in oysters. |
format |
Text |
author |
Yang, Min Tong, Lihui Wang, Shanshan Liu, Nan Zhao, Feng Sun, Yong Sun, Guohui Zhou, Deqing |
author_facet |
Yang, Min Tong, Lihui Wang, Shanshan Liu, Nan Zhao, Feng Sun, Yong Sun, Guohui Zhou, Deqing |
author_sort |
Yang, Min |
title |
Gut Microbiota and Transcriptomics Reveal the Effect of Human Norovirus Bioaccumulation on Oysters (Crassostrea gigas) |
title_short |
Gut Microbiota and Transcriptomics Reveal the Effect of Human Norovirus Bioaccumulation on Oysters (Crassostrea gigas) |
title_full |
Gut Microbiota and Transcriptomics Reveal the Effect of Human Norovirus Bioaccumulation on Oysters (Crassostrea gigas) |
title_fullStr |
Gut Microbiota and Transcriptomics Reveal the Effect of Human Norovirus Bioaccumulation on Oysters (Crassostrea gigas) |
title_full_unstemmed |
Gut Microbiota and Transcriptomics Reveal the Effect of Human Norovirus Bioaccumulation on Oysters (Crassostrea gigas) |
title_sort |
gut microbiota and transcriptomics reveal the effect of human norovirus bioaccumulation on oysters (crassostrea gigas) |
publisher |
American Society for Microbiology |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9431538/ http://www.ncbi.nlm.nih.gov/pubmed/35867424 https://doi.org/10.1128/spectrum.00161-22 |
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Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Microbiol Spectr |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9431538/ http://www.ncbi.nlm.nih.gov/pubmed/35867424 http://dx.doi.org/10.1128/spectrum.00161-22 |
op_rights |
Copyright © 2022 Yang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
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CC-BY |
op_doi |
https://doi.org/10.1128/spectrum.00161-22 |
container_title |
Microbiology Spectrum |
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10 |
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4 |
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1766394638587920384 |