Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions
Increased research attention is being given to bacterial diversity associated with lichens. Rock tripe lichens (Umbilicariaceae) were collected from two distinct Antarctic biological regions, the continental region near the Japanese Antarctic station (Syowa Station) and the maritime Antarctic South...
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ftpubmed:oai:pubmedcentral.nih.gov:9409739 2023-05-15T13:53:29+02:00 Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions He, Zichen Naganuma, Takeshi Nakai, Ryosuke Imura, Satoshi Tsujimoto, Megumu Convey, Peter 2022-08-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409739/ https://doi.org/10.3390/jof8080817 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409739/ http://dx.doi.org/10.3390/jof8080817 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY J Fungi (Basel) Article Text 2022 ftpubmed https://doi.org/10.3390/jof8080817 2022-08-28T01:28:54Z Increased research attention is being given to bacterial diversity associated with lichens. Rock tripe lichens (Umbilicariaceae) were collected from two distinct Antarctic biological regions, the continental region near the Japanese Antarctic station (Syowa Station) and the maritime Antarctic South Orkney Islands (Signy Island), in order to compare their bacterial floras and potential metabolism. Bulk DNA extracted from the lichen samples was used to amplify the 18S rRNA gene and the V3-V4 region of the 16S rRNA gene, whose amplicons were Sanger- and MiSeq-sequenced, respectively. The fungal and algal partners represented members of the ascomycete genus Umbilicaria and the green algal genus Trebouxia, based on 18S rRNA gene sequences. The V3-V4 sequences were grouped into operational taxonomic units (OTUs), which were assigned to eight bacterial phyla, Acidobacteriota, Actinomyceota, Armatimonadota, Bacteroidota, Cyanobacteria, Deinococcota, Pseudomonadota and the candidate phylum Saccharibacteria (also known as TM7), commonly present in all samples. The OTU floras of the two biological regions were clearly distinct, with regional biomarker genera, such as Mucilaginibacter and Gluconacetobacter, respectively. The OTU-based metabolism analysis predicted higher membrane transport activities in the maritime Antarctic OTUs, probably influenced by the sampling area’s warmer maritime climatic setting. Text Antarc* Antarctic Signy Island South Orkney Islands PubMed Central (PMC) Antarctic Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) South Orkney Islands ENVELOPE(-45.500,-45.500,-60.583,-60.583) Syowa Station Journal of Fungi 8 8 817 |
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PubMed Central (PMC) |
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ftpubmed |
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English |
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Article |
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Article He, Zichen Naganuma, Takeshi Nakai, Ryosuke Imura, Satoshi Tsujimoto, Megumu Convey, Peter Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
topic_facet |
Article |
description |
Increased research attention is being given to bacterial diversity associated with lichens. Rock tripe lichens (Umbilicariaceae) were collected from two distinct Antarctic biological regions, the continental region near the Japanese Antarctic station (Syowa Station) and the maritime Antarctic South Orkney Islands (Signy Island), in order to compare their bacterial floras and potential metabolism. Bulk DNA extracted from the lichen samples was used to amplify the 18S rRNA gene and the V3-V4 region of the 16S rRNA gene, whose amplicons were Sanger- and MiSeq-sequenced, respectively. The fungal and algal partners represented members of the ascomycete genus Umbilicaria and the green algal genus Trebouxia, based on 18S rRNA gene sequences. The V3-V4 sequences were grouped into operational taxonomic units (OTUs), which were assigned to eight bacterial phyla, Acidobacteriota, Actinomyceota, Armatimonadota, Bacteroidota, Cyanobacteria, Deinococcota, Pseudomonadota and the candidate phylum Saccharibacteria (also known as TM7), commonly present in all samples. The OTU floras of the two biological regions were clearly distinct, with regional biomarker genera, such as Mucilaginibacter and Gluconacetobacter, respectively. The OTU-based metabolism analysis predicted higher membrane transport activities in the maritime Antarctic OTUs, probably influenced by the sampling area’s warmer maritime climatic setting. |
format |
Text |
author |
He, Zichen Naganuma, Takeshi Nakai, Ryosuke Imura, Satoshi Tsujimoto, Megumu Convey, Peter |
author_facet |
He, Zichen Naganuma, Takeshi Nakai, Ryosuke Imura, Satoshi Tsujimoto, Megumu Convey, Peter |
author_sort |
He, Zichen |
title |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_short |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_full |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_fullStr |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_full_unstemmed |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_sort |
microbiomic analysis of bacteria associated with rock tripe lichens in continental and maritime antarctic regions |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409739/ https://doi.org/10.3390/jof8080817 |
long_lat |
ENVELOPE(-45.595,-45.595,-60.708,-60.708) ENVELOPE(-45.500,-45.500,-60.583,-60.583) |
geographic |
Antarctic Signy Island South Orkney Islands Syowa Station |
geographic_facet |
Antarctic Signy Island South Orkney Islands Syowa Station |
genre |
Antarc* Antarctic Signy Island South Orkney Islands |
genre_facet |
Antarc* Antarctic Signy Island South Orkney Islands |
op_source |
J Fungi (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9409739/ http://dx.doi.org/10.3390/jof8080817 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/jof8080817 |
container_title |
Journal of Fungi |
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8 |
container_issue |
8 |
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817 |
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1766258636606144512 |