Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil
Degradation of petroleum hydrocarbons was monitored in microcosms with diesel fuel-contaminated Arctic tundra soil incubated for 48 days at low temperatures (−5, 0, and 7°C). An additional treatment was incubation for alternating 24-h periods at 7 and −5°C. Hydrocarbons were biodegraded at or above...
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American Society for Microbiology
2001
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93278 http://www.ncbi.nlm.nih.gov/pubmed/11679333 https://doi.org/10.1128/AEM.67.11.5107-5112.2001 |
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ftpubmed:oai:pubmedcentral.nih.gov:93278 2023-05-15T14:57:52+02:00 Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil Eriksson, Mikael Ka, Jong-Ok Mohn, William W. 2001-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93278 http://www.ncbi.nlm.nih.gov/pubmed/11679333 https://doi.org/10.1128/AEM.67.11.5107-5112.2001 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93278 http://www.ncbi.nlm.nih.gov/pubmed/11679333 http://dx.doi.org/10.1128/AEM.67.11.5107-5112.2001 Copyright © 2001, American Society for Microbiology Environmental Microbiology and Biodegradation Text 2001 ftpubmed https://doi.org/10.1128/AEM.67.11.5107-5112.2001 2013-08-29T09:24:27Z Degradation of petroleum hydrocarbons was monitored in microcosms with diesel fuel-contaminated Arctic tundra soil incubated for 48 days at low temperatures (−5, 0, and 7°C). An additional treatment was incubation for alternating 24-h periods at 7 and −5°C. Hydrocarbons were biodegraded at or above 0°C, and freeze-thaw cycles may have actually stimulated hydrocarbon biodegradation. Total petroleum hydrocarbon (TPH) removal over 48 days in the 7, 0, and 7 and −5°C treatments, respectively, was 450, 300, and 600 μg/g of soil. No TPH removal was observed at −5°C. Total carbon dioxide production suggested that TPH removal was due to biological mineralization. Bacterial metabolic activity, indicated by RNA/DNA ratios, was higher in the middle of the experiment (day 21) than at the start, in agreement with measured hydrocarbon removal and carbon dioxide production activities. The total numbers of culturable heterotrophs and of hydrocarbon degraders did not change significantly over the 48 days of incubation in any of the treatments. At the end of the experiment, bacterial community structure, evaluated by ribosomal intergenic spacer length analysis, was very similar in all of the treatments but the alternating 7 and −5°C treatment. Text Arctic Tundra PubMed Central (PMC) Arctic Applied and Environmental Microbiology 67 11 5107 5112 |
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language |
English |
topic |
Environmental Microbiology and Biodegradation |
spellingShingle |
Environmental Microbiology and Biodegradation Eriksson, Mikael Ka, Jong-Ok Mohn, William W. Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
topic_facet |
Environmental Microbiology and Biodegradation |
description |
Degradation of petroleum hydrocarbons was monitored in microcosms with diesel fuel-contaminated Arctic tundra soil incubated for 48 days at low temperatures (−5, 0, and 7°C). An additional treatment was incubation for alternating 24-h periods at 7 and −5°C. Hydrocarbons were biodegraded at or above 0°C, and freeze-thaw cycles may have actually stimulated hydrocarbon biodegradation. Total petroleum hydrocarbon (TPH) removal over 48 days in the 7, 0, and 7 and −5°C treatments, respectively, was 450, 300, and 600 μg/g of soil. No TPH removal was observed at −5°C. Total carbon dioxide production suggested that TPH removal was due to biological mineralization. Bacterial metabolic activity, indicated by RNA/DNA ratios, was higher in the middle of the experiment (day 21) than at the start, in agreement with measured hydrocarbon removal and carbon dioxide production activities. The total numbers of culturable heterotrophs and of hydrocarbon degraders did not change significantly over the 48 days of incubation in any of the treatments. At the end of the experiment, bacterial community structure, evaluated by ribosomal intergenic spacer length analysis, was very similar in all of the treatments but the alternating 7 and −5°C treatment. |
format |
Text |
author |
Eriksson, Mikael Ka, Jong-Ok Mohn, William W. |
author_facet |
Eriksson, Mikael Ka, Jong-Ok Mohn, William W. |
author_sort |
Eriksson, Mikael |
title |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_short |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_full |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_fullStr |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_full_unstemmed |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_sort |
effects of low temperature and freeze-thaw cycles on hydrocarbon biodegradation in arctic tundra soil |
publisher |
American Society for Microbiology |
publishDate |
2001 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93278 http://www.ncbi.nlm.nih.gov/pubmed/11679333 https://doi.org/10.1128/AEM.67.11.5107-5112.2001 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Tundra |
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Arctic Tundra |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93278 http://www.ncbi.nlm.nih.gov/pubmed/11679333 http://dx.doi.org/10.1128/AEM.67.11.5107-5112.2001 |
op_rights |
Copyright © 2001, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.67.11.5107-5112.2001 |
container_title |
Applied and Environmental Microbiology |
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67 |
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11 |
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5107 |
op_container_end_page |
5112 |
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1766329968356229120 |