Exploring Sea Lice Vaccines against Early Stages of Infestation in Atlantic Salmon (Salmo salar)
The sea louse Caligus rogercresseyi genome has opened the opportunity to apply the reverse vaccinology strategy for identifying antigens with potential effects on lice development and its application in sea lice control. This study aimed to explore the efficacy of three sea lice vaccines against the...
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ftpubmed:oai:pubmedcentral.nih.gov:9324576 2023-05-15T15:30:55+02:00 Exploring Sea Lice Vaccines against Early Stages of Infestation in Atlantic Salmon (Salmo salar) Casuso, Antonio Valenzuela-Muñoz, Valentina Benavente, Bárbara P. Valenzuela-Miranda, Diego Gallardo-Escárate, Cristian 2022-07-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324576/ http://www.ncbi.nlm.nih.gov/pubmed/35891227 https://doi.org/10.3390/vaccines10071063 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324576/ http://www.ncbi.nlm.nih.gov/pubmed/35891227 http://dx.doi.org/10.3390/vaccines10071063 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Vaccines (Basel) Article Text 2022 ftpubmed https://doi.org/10.3390/vaccines10071063 2022-07-31T03:20:21Z The sea louse Caligus rogercresseyi genome has opened the opportunity to apply the reverse vaccinology strategy for identifying antigens with potential effects on lice development and its application in sea lice control. This study aimed to explore the efficacy of three sea lice vaccines against the early stage of infestation, assessing the transcriptome modulation of immunized Atlantic salmon. Therein, three experimental groups of Salmo salar (Atlantic salmon) were vaccinated with the recombinant proteins: Peritrophin (prototype A), Cathepsin (prototype B), and the mix of them (prototype C), respectively. Sea lice infestation was evaluated during chalimus I-II, the early-infective stages attached at 7-days post infestation. In parallel, head kidney and skin tissue samples were taken for mRNA Illumina sequencing. Relative expression analyses of genes were conducted to identify immune responses, iron transport, and stress responses associated with the tested vaccines during the early stages of sea lice infection. The vaccine prototypes A, B, and C reduced the parasite burden by 24, 44, and 52% compared with the control group. In addition, the RNA-Seq analysis exhibited a prototype-dependent transcriptome modulation. The high expression differences were observed in genes associated with metal ion binding, molecular processes, and energy production. The findings suggest a balance between the host’s inflammatory response and metabolic process in vaccinated fish, increasing their transcriptional activity, which can alter the early host–parasite interactions. This study uncovers molecular responses produced by three vaccine prototypes at the early stages of infestation, providing new knowledge for sea lice control in the salmon aquaculture. Text Atlantic salmon Salmo salar PubMed Central (PMC) Vaccines 10 7 1063 |
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Article Casuso, Antonio Valenzuela-Muñoz, Valentina Benavente, Bárbara P. Valenzuela-Miranda, Diego Gallardo-Escárate, Cristian Exploring Sea Lice Vaccines against Early Stages of Infestation in Atlantic Salmon (Salmo salar) |
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The sea louse Caligus rogercresseyi genome has opened the opportunity to apply the reverse vaccinology strategy for identifying antigens with potential effects on lice development and its application in sea lice control. This study aimed to explore the efficacy of three sea lice vaccines against the early stage of infestation, assessing the transcriptome modulation of immunized Atlantic salmon. Therein, three experimental groups of Salmo salar (Atlantic salmon) were vaccinated with the recombinant proteins: Peritrophin (prototype A), Cathepsin (prototype B), and the mix of them (prototype C), respectively. Sea lice infestation was evaluated during chalimus I-II, the early-infective stages attached at 7-days post infestation. In parallel, head kidney and skin tissue samples were taken for mRNA Illumina sequencing. Relative expression analyses of genes were conducted to identify immune responses, iron transport, and stress responses associated with the tested vaccines during the early stages of sea lice infection. The vaccine prototypes A, B, and C reduced the parasite burden by 24, 44, and 52% compared with the control group. In addition, the RNA-Seq analysis exhibited a prototype-dependent transcriptome modulation. The high expression differences were observed in genes associated with metal ion binding, molecular processes, and energy production. The findings suggest a balance between the host’s inflammatory response and metabolic process in vaccinated fish, increasing their transcriptional activity, which can alter the early host–parasite interactions. This study uncovers molecular responses produced by three vaccine prototypes at the early stages of infestation, providing new knowledge for sea lice control in the salmon aquaculture. |
format |
Text |
author |
Casuso, Antonio Valenzuela-Muñoz, Valentina Benavente, Bárbara P. Valenzuela-Miranda, Diego Gallardo-Escárate, Cristian |
author_facet |
Casuso, Antonio Valenzuela-Muñoz, Valentina Benavente, Bárbara P. Valenzuela-Miranda, Diego Gallardo-Escárate, Cristian |
author_sort |
Casuso, Antonio |
title |
Exploring Sea Lice Vaccines against Early Stages of Infestation in Atlantic Salmon (Salmo salar) |
title_short |
Exploring Sea Lice Vaccines against Early Stages of Infestation in Atlantic Salmon (Salmo salar) |
title_full |
Exploring Sea Lice Vaccines against Early Stages of Infestation in Atlantic Salmon (Salmo salar) |
title_fullStr |
Exploring Sea Lice Vaccines against Early Stages of Infestation in Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Exploring Sea Lice Vaccines against Early Stages of Infestation in Atlantic Salmon (Salmo salar) |
title_sort |
exploring sea lice vaccines against early stages of infestation in atlantic salmon (salmo salar) |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324576/ http://www.ncbi.nlm.nih.gov/pubmed/35891227 https://doi.org/10.3390/vaccines10071063 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Vaccines (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324576/ http://www.ncbi.nlm.nih.gov/pubmed/35891227 http://dx.doi.org/10.3390/vaccines10071063 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/vaccines10071063 |
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Vaccines |
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10 |
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1063 |
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