Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei
The IgM and IgT classes were previously identified and characterized in the Antarctic teleost Trematomus bernacchii, a species belonging to the Perciform suborder Notothenoidei. Herein, we characterized the gene encoding the polymeric immunoglobulin receptor (pIgR) in the same species and compared i...
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ftpubmed:oai:pubmedcentral.nih.gov:9321927 2023-05-15T13:30:32+02:00 Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei Ametrano, Alessia Picchietti, Simona Guerra, Laura Giacomelli, Stefano Oreste, Umberto Coscia, Maria Rosaria 2022-07-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321927/ http://www.ncbi.nlm.nih.gov/pubmed/35887127 https://doi.org/10.3390/ijms23147783 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321927/ http://www.ncbi.nlm.nih.gov/pubmed/35887127 http://dx.doi.org/10.3390/ijms23147783 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Int J Mol Sci Article Text 2022 ftpubmed https://doi.org/10.3390/ijms23147783 2022-07-31T03:13:43Z The IgM and IgT classes were previously identified and characterized in the Antarctic teleost Trematomus bernacchii, a species belonging to the Perciform suborder Notothenoidei. Herein, we characterized the gene encoding the polymeric immunoglobulin receptor (pIgR) in the same species and compared it to the pIgR of multiple teleost species belonging to five perciform suborders, including 11 Antarctic and 1 non-Antarctic (Cottoperca gobio) notothenioid species, the latter living in the less-cold peri-Antarctic sea. Antarctic pIgR genes displayed particularly long introns marked by sites of transposable elements and transcription factors. Furthermore, analysis of T. bernacchii pIgR cDNA unveiled multiple amino acid substitutions unique to the Antarctic species, all introducing adaptive features, including N-glycosylation sequons. Interestingly, C. gobio shared most features with the other perciforms rather than with the cold-adapted relatives. T. bernacchii pIgR transcripts were predominantly expressed in mucosal tissues, as indicated by q-PCR and in situ hybridization analysis. These results suggest that in cold-adapted species, pIgR preserved its fundamental role in mucosal immune defense, although remarkable gene structure modifications occurred. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic International Journal of Molecular Sciences 23 14 7783 |
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Article Ametrano, Alessia Picchietti, Simona Guerra, Laura Giacomelli, Stefano Oreste, Umberto Coscia, Maria Rosaria Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
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The IgM and IgT classes were previously identified and characterized in the Antarctic teleost Trematomus bernacchii, a species belonging to the Perciform suborder Notothenoidei. Herein, we characterized the gene encoding the polymeric immunoglobulin receptor (pIgR) in the same species and compared it to the pIgR of multiple teleost species belonging to five perciform suborders, including 11 Antarctic and 1 non-Antarctic (Cottoperca gobio) notothenioid species, the latter living in the less-cold peri-Antarctic sea. Antarctic pIgR genes displayed particularly long introns marked by sites of transposable elements and transcription factors. Furthermore, analysis of T. bernacchii pIgR cDNA unveiled multiple amino acid substitutions unique to the Antarctic species, all introducing adaptive features, including N-glycosylation sequons. Interestingly, C. gobio shared most features with the other perciforms rather than with the cold-adapted relatives. T. bernacchii pIgR transcripts were predominantly expressed in mucosal tissues, as indicated by q-PCR and in situ hybridization analysis. These results suggest that in cold-adapted species, pIgR preserved its fundamental role in mucosal immune defense, although remarkable gene structure modifications occurred. |
format |
Text |
author |
Ametrano, Alessia Picchietti, Simona Guerra, Laura Giacomelli, Stefano Oreste, Umberto Coscia, Maria Rosaria |
author_facet |
Ametrano, Alessia Picchietti, Simona Guerra, Laura Giacomelli, Stefano Oreste, Umberto Coscia, Maria Rosaria |
author_sort |
Ametrano, Alessia |
title |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_short |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_full |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_fullStr |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_full_unstemmed |
Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Reveals Distinctive Features of Cold-Adapted Notothenioidei |
title_sort |
comparative analysis of the pigr gene from the antarctic teleost trematomus bernacchii reveals distinctive features of cold-adapted notothenioidei |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321927/ http://www.ncbi.nlm.nih.gov/pubmed/35887127 https://doi.org/10.3390/ijms23147783 |
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Antarctic The Antarctic |
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Antarctic The Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
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Int J Mol Sci |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321927/ http://www.ncbi.nlm.nih.gov/pubmed/35887127 http://dx.doi.org/10.3390/ijms23147783 |
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© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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https://doi.org/10.3390/ijms23147783 |
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International Journal of Molecular Sciences |
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23 |
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14 |
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7783 |
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1766009702710247424 |