Soil substrate culturing approaches recover diverse members of Actinomycetota from desert soils of Herring Island, East Antarctica
Antimicrobial resistance is an escalating health crisis requiring urgent action. Most antimicrobials are natural products (NPs) sourced from Actinomycetota, particularly the Streptomyces. Underexplored and extreme environments are predicted to harbour novel microorganisms with the capacity to synthe...
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ftpubmed:oai:pubmedcentral.nih.gov:9279279 2023-05-15T13:50:20+02:00 Soil substrate culturing approaches recover diverse members of Actinomycetota from desert soils of Herring Island, East Antarctica Benaud, Nicole Chelliah, Devan S. Wong, Sin Yin Ferrari, Belinda C. 2022-07-13 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279279/ http://www.ncbi.nlm.nih.gov/pubmed/35829965 https://doi.org/10.1007/s00792-022-01271-2 en eng Springer Japan http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279279/ http://www.ncbi.nlm.nih.gov/pubmed/35829965 http://dx.doi.org/10.1007/s00792-022-01271-2 © Crown 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Extremophiles Original Paper Text 2022 ftpubmed https://doi.org/10.1007/s00792-022-01271-2 2022-07-31T01:25:30Z Antimicrobial resistance is an escalating health crisis requiring urgent action. Most antimicrobials are natural products (NPs) sourced from Actinomycetota, particularly the Streptomyces. Underexplored and extreme environments are predicted to harbour novel microorganisms with the capacity to synthesise unique metabolites. Herring Island is a barren and rocky cold desert in East Antarctica, remote from anthropogenic impact. We aimed to recover rare and cold-adapted NP-producing bacteria, by employing two culturing methods which mimic the natural environment: direct soil culturing and the soil substrate membrane system. First, we analysed 16S rRNA gene amplicon sequencing data from 18 Herring Island soils and selected the soil sample with the highest Actinomycetota relative abundance (78%) for culturing experiments. We isolated 166 strains across three phyla, including novel and rare strains, with 94% of strains belonging to the Actinomycetota. These strains encompassed thirty-five ‘species’ groups, 18 of which were composed of Streptomyces strains. We screened representative strains for genes which encode polyketide synthases and non-ribosomal peptide synthetases, indicating that 69% have the capacity to synthesise polyketide and non-ribosomal peptide NPs. Fourteen Streptomyces strains displayed antimicrobial activity against selected bacterial and yeast pathogens using an in situ assay. Our results confirm that the cold-adapted bacteria of the harsh East Antarctic deserts are worthy targets in the search for bioactive compounds. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00792-022-01271-2. Text Antarc* Antarctic Antarctica East Antarctica Herring Island PubMed Central (PMC) Antarctic East Antarctica Herring Island ENVELOPE(110.633,110.633,-66.406,-66.406) Extremophiles 26 2 |
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English |
topic |
Original Paper |
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Original Paper Benaud, Nicole Chelliah, Devan S. Wong, Sin Yin Ferrari, Belinda C. Soil substrate culturing approaches recover diverse members of Actinomycetota from desert soils of Herring Island, East Antarctica |
topic_facet |
Original Paper |
description |
Antimicrobial resistance is an escalating health crisis requiring urgent action. Most antimicrobials are natural products (NPs) sourced from Actinomycetota, particularly the Streptomyces. Underexplored and extreme environments are predicted to harbour novel microorganisms with the capacity to synthesise unique metabolites. Herring Island is a barren and rocky cold desert in East Antarctica, remote from anthropogenic impact. We aimed to recover rare and cold-adapted NP-producing bacteria, by employing two culturing methods which mimic the natural environment: direct soil culturing and the soil substrate membrane system. First, we analysed 16S rRNA gene amplicon sequencing data from 18 Herring Island soils and selected the soil sample with the highest Actinomycetota relative abundance (78%) for culturing experiments. We isolated 166 strains across three phyla, including novel and rare strains, with 94% of strains belonging to the Actinomycetota. These strains encompassed thirty-five ‘species’ groups, 18 of which were composed of Streptomyces strains. We screened representative strains for genes which encode polyketide synthases and non-ribosomal peptide synthetases, indicating that 69% have the capacity to synthesise polyketide and non-ribosomal peptide NPs. Fourteen Streptomyces strains displayed antimicrobial activity against selected bacterial and yeast pathogens using an in situ assay. Our results confirm that the cold-adapted bacteria of the harsh East Antarctic deserts are worthy targets in the search for bioactive compounds. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00792-022-01271-2. |
format |
Text |
author |
Benaud, Nicole Chelliah, Devan S. Wong, Sin Yin Ferrari, Belinda C. |
author_facet |
Benaud, Nicole Chelliah, Devan S. Wong, Sin Yin Ferrari, Belinda C. |
author_sort |
Benaud, Nicole |
title |
Soil substrate culturing approaches recover diverse members of Actinomycetota from desert soils of Herring Island, East Antarctica |
title_short |
Soil substrate culturing approaches recover diverse members of Actinomycetota from desert soils of Herring Island, East Antarctica |
title_full |
Soil substrate culturing approaches recover diverse members of Actinomycetota from desert soils of Herring Island, East Antarctica |
title_fullStr |
Soil substrate culturing approaches recover diverse members of Actinomycetota from desert soils of Herring Island, East Antarctica |
title_full_unstemmed |
Soil substrate culturing approaches recover diverse members of Actinomycetota from desert soils of Herring Island, East Antarctica |
title_sort |
soil substrate culturing approaches recover diverse members of actinomycetota from desert soils of herring island, east antarctica |
publisher |
Springer Japan |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279279/ http://www.ncbi.nlm.nih.gov/pubmed/35829965 https://doi.org/10.1007/s00792-022-01271-2 |
long_lat |
ENVELOPE(110.633,110.633,-66.406,-66.406) |
geographic |
Antarctic East Antarctica Herring Island |
geographic_facet |
Antarctic East Antarctica Herring Island |
genre |
Antarc* Antarctic Antarctica East Antarctica Herring Island |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica Herring Island |
op_source |
Extremophiles |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279279/ http://www.ncbi.nlm.nih.gov/pubmed/35829965 http://dx.doi.org/10.1007/s00792-022-01271-2 |
op_rights |
© Crown 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00792-022-01271-2 |
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Extremophiles |
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26 |
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2 |
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1766253363162251264 |