Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure
A reusable support system for the immobilization of lipases is developed using hybrid polymer-inorganic core shell nanoparticles. The biocatalyst core consists of a silica nanoparticle. PMMA is grafted from the nanoparticle as polymer brush via ARGET ATRP (activator regenerated by electron transfer...
Published in: | Applied Biochemistry and Biotechnology |
---|---|
Main Authors: | , , , , |
Format: | Text |
Language: | English |
Published: |
Springer US
2022
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270307/ http://www.ncbi.nlm.nih.gov/pubmed/35357660 https://doi.org/10.1007/s12010-022-03913-9 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:9270307 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:9270307 2023-05-15T13:52:49+02:00 Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure Wunschik, Dennis Sebastian Lorenz, André Ingenbosch, Kim Nadine Gutmann, Jochen Stefan Hoffmann-Jacobsen, Kerstin 2022-03-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270307/ http://www.ncbi.nlm.nih.gov/pubmed/35357660 https://doi.org/10.1007/s12010-022-03913-9 en eng Springer US http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270307/ http://www.ncbi.nlm.nih.gov/pubmed/35357660 http://dx.doi.org/10.1007/s12010-022-03913-9 © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Appl Biochem Biotechnol Original Article Text 2022 ftpubmed https://doi.org/10.1007/s12010-022-03913-9 2022-07-31T01:03:04Z A reusable support system for the immobilization of lipases is developed using hybrid polymer-inorganic core shell nanoparticles. The biocatalyst core consists of a silica nanoparticle. PMMA is grafted from the nanoparticle as polymer brush via ARGET ATRP (activator regenerated by electron transfer atom transfer radical polymerization), which allows defining the surface properties by chemical synthesis conditions. Lipase B from Candida antarctica is immobilized on the hybrid particles. The activity and stability of the biocatalyst are analyzed by spectroscopic activity analysis. It is shown that the hydrophobic PMMA brushes provide an activating surface for the lipase giving a higher specific activity than the enzyme in solution. Varying the surface structure from disordered to ordered polymer brushes reveals that the reusability of the biocatalyst is more effectively optimized by the surface structure than by the introduction of crosslinking with glutaraldehyde (GDA). The developed immobilization system is highly suitable for biocatalysis in non-native media which is shown by a transesterification assay in isopropyl alcohol and an esterification reaction in n-heptane. Text Antarc* Antarctica PubMed Central (PMC) Applied Biochemistry and Biotechnology 194 8 3384 3399 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Original Article |
spellingShingle |
Original Article Wunschik, Dennis Sebastian Lorenz, André Ingenbosch, Kim Nadine Gutmann, Jochen Stefan Hoffmann-Jacobsen, Kerstin Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure |
topic_facet |
Original Article |
description |
A reusable support system for the immobilization of lipases is developed using hybrid polymer-inorganic core shell nanoparticles. The biocatalyst core consists of a silica nanoparticle. PMMA is grafted from the nanoparticle as polymer brush via ARGET ATRP (activator regenerated by electron transfer atom transfer radical polymerization), which allows defining the surface properties by chemical synthesis conditions. Lipase B from Candida antarctica is immobilized on the hybrid particles. The activity and stability of the biocatalyst are analyzed by spectroscopic activity analysis. It is shown that the hydrophobic PMMA brushes provide an activating surface for the lipase giving a higher specific activity than the enzyme in solution. Varying the surface structure from disordered to ordered polymer brushes reveals that the reusability of the biocatalyst is more effectively optimized by the surface structure than by the introduction of crosslinking with glutaraldehyde (GDA). The developed immobilization system is highly suitable for biocatalysis in non-native media which is shown by a transesterification assay in isopropyl alcohol and an esterification reaction in n-heptane. |
format |
Text |
author |
Wunschik, Dennis Sebastian Lorenz, André Ingenbosch, Kim Nadine Gutmann, Jochen Stefan Hoffmann-Jacobsen, Kerstin |
author_facet |
Wunschik, Dennis Sebastian Lorenz, André Ingenbosch, Kim Nadine Gutmann, Jochen Stefan Hoffmann-Jacobsen, Kerstin |
author_sort |
Wunschik, Dennis Sebastian |
title |
Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure |
title_short |
Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure |
title_full |
Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure |
title_fullStr |
Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure |
title_full_unstemmed |
Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure |
title_sort |
activation and stabilization of lipase b from candida antarctica by immobilization on polymer brushes with optimized surface structure |
publisher |
Springer US |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270307/ http://www.ncbi.nlm.nih.gov/pubmed/35357660 https://doi.org/10.1007/s12010-022-03913-9 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Appl Biochem Biotechnol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270307/ http://www.ncbi.nlm.nih.gov/pubmed/35357660 http://dx.doi.org/10.1007/s12010-022-03913-9 |
op_rights |
© The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s12010-022-03913-9 |
container_title |
Applied Biochemistry and Biotechnology |
container_volume |
194 |
container_issue |
8 |
container_start_page |
3384 |
op_container_end_page |
3399 |
_version_ |
1766257573738053632 |