Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard)
Fluorescence in situ hybridization (FISH) and rRNA slot blot hybridization with 16S rRNA-targeted oligonucleotide probes were used to investigate the phylogenetic composition of a marine Arctic sediment (Svalbard). FISH resulted in the detection of a large fraction of microbes living in the top 5 cm...
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ftpubmed:oai:pubmedcentral.nih.gov:92590 2023-05-15T14:59:58+02:00 Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard) Ravenschlag, Katrin Sahm, Kerstin Amann, Rudolf 2001-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92590 http://www.ncbi.nlm.nih.gov/pubmed/11133470 https://doi.org/10.1128/AEM.67.1.387-395.2001 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92590 http://www.ncbi.nlm.nih.gov/pubmed/11133470 http://dx.doi.org/10.1128/AEM.67.1.387-395.2001 Copyright © 2001, American Society for Microbiology Microbial Ecology Text 2001 ftpubmed https://doi.org/10.1128/AEM.67.1.387-395.2001 2013-08-29T09:22:07Z Fluorescence in situ hybridization (FISH) and rRNA slot blot hybridization with 16S rRNA-targeted oligonucleotide probes were used to investigate the phylogenetic composition of a marine Arctic sediment (Svalbard). FISH resulted in the detection of a large fraction of microbes living in the top 5 cm of the sediment. Up to 65.4% ± 7.5% of total DAPI (4′,6′-diamidino-2-phenylindole) cell counts hybridized to the bacterial probe EUB338, and up to 4.9% ± 1.5% hybridized to the archaeal probe ARCH915. Besides δ-proteobacterial sulfate-reducing bacteria (up to 16% 52) members of the Cytophaga-Flavobacterium cluster were the most abundant group detected in this sediment, accounting for up to 12.8% of total DAPI cell counts and up to 6.1% of prokaryotic rRNA. Furthermore, members of the order Planctomycetales accounted for up to 3.9% of total cell counts. In accordance with previous studies, these findings support the hypothesis that these bacterial groups are not simply settling with organic matter from the pelagic zone but are indigenous to the anoxic zones of marine sediments. Members of the γ-proteobacteria also constituted a significant fraction in this sediment (6.1% ± 2.5% of total cell counts, 14.4% ± 3.6% of prokaryotic rRNA). A new probe (GAM660) specific for sequences affiliated with free-living or endosymbiotic sulfur-oxidizing bacteria was developed. A significant number of cells was detected by this probe (2.1% ± 0.7% of total DAPI cell counts, 13.2% ± 4.6% of prokaryotic rRNA), showing no clear zonation along the vertical profile. Gram-positive bacteria and the β-proteobacteria were near the detection limit in all sediments. Text Arctic Svalbard PubMed Central (PMC) Arctic Svalbard Applied and Environmental Microbiology 67 1 387 395 |
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Open Polar |
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language |
English |
topic |
Microbial Ecology |
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Microbial Ecology Ravenschlag, Katrin Sahm, Kerstin Amann, Rudolf Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard) |
topic_facet |
Microbial Ecology |
description |
Fluorescence in situ hybridization (FISH) and rRNA slot blot hybridization with 16S rRNA-targeted oligonucleotide probes were used to investigate the phylogenetic composition of a marine Arctic sediment (Svalbard). FISH resulted in the detection of a large fraction of microbes living in the top 5 cm of the sediment. Up to 65.4% ± 7.5% of total DAPI (4′,6′-diamidino-2-phenylindole) cell counts hybridized to the bacterial probe EUB338, and up to 4.9% ± 1.5% hybridized to the archaeal probe ARCH915. Besides δ-proteobacterial sulfate-reducing bacteria (up to 16% 52) members of the Cytophaga-Flavobacterium cluster were the most abundant group detected in this sediment, accounting for up to 12.8% of total DAPI cell counts and up to 6.1% of prokaryotic rRNA. Furthermore, members of the order Planctomycetales accounted for up to 3.9% of total cell counts. In accordance with previous studies, these findings support the hypothesis that these bacterial groups are not simply settling with organic matter from the pelagic zone but are indigenous to the anoxic zones of marine sediments. Members of the γ-proteobacteria also constituted a significant fraction in this sediment (6.1% ± 2.5% of total cell counts, 14.4% ± 3.6% of prokaryotic rRNA). A new probe (GAM660) specific for sequences affiliated with free-living or endosymbiotic sulfur-oxidizing bacteria was developed. A significant number of cells was detected by this probe (2.1% ± 0.7% of total DAPI cell counts, 13.2% ± 4.6% of prokaryotic rRNA), showing no clear zonation along the vertical profile. Gram-positive bacteria and the β-proteobacteria were near the detection limit in all sediments. |
format |
Text |
author |
Ravenschlag, Katrin Sahm, Kerstin Amann, Rudolf |
author_facet |
Ravenschlag, Katrin Sahm, Kerstin Amann, Rudolf |
author_sort |
Ravenschlag, Katrin |
title |
Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard) |
title_short |
Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard) |
title_full |
Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard) |
title_fullStr |
Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard) |
title_full_unstemmed |
Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard) |
title_sort |
quantitative molecular analysis of the microbial community in marine arctic sediments (svalbard) |
publisher |
American Society for Microbiology |
publishDate |
2001 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92590 http://www.ncbi.nlm.nih.gov/pubmed/11133470 https://doi.org/10.1128/AEM.67.1.387-395.2001 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Svalbard |
genre_facet |
Arctic Svalbard |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92590 http://www.ncbi.nlm.nih.gov/pubmed/11133470 http://dx.doi.org/10.1128/AEM.67.1.387-395.2001 |
op_rights |
Copyright © 2001, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.67.1.387-395.2001 |
container_title |
Applied and Environmental Microbiology |
container_volume |
67 |
container_issue |
1 |
container_start_page |
387 |
op_container_end_page |
395 |
_version_ |
1766332080878256128 |