Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome
Alpha mannose-oligosaccharide (MOS) prebiotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and their associated metabolism. Bio-Mos is a co...
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ftpubmed:oai:pubmedcentral.nih.gov:9241627 2023-05-15T15:32:04+02:00 Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome Kazlauskaite, Raminta Cheaib, Bachar Humble, Joseph Heys, Chloe Ijaz, Umer Zeeshan Connelly, Stephanie Sloan, William T. Russell, Julie Martinez-Rubio, Laura Sweetman, John Kitts, Alex McGinnity, Philip Lyons, Philip Llewellyn, Martin S. 2022-05-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241627/ http://www.ncbi.nlm.nih.gov/pubmed/35532227 https://doi.org/10.1128/spectrum.01953-21 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241627/ http://www.ncbi.nlm.nih.gov/pubmed/35532227 http://dx.doi.org/10.1128/spectrum.01953-21 Copyright © 2022 Kazlauskaite et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . CC-BY Microbiol Spectr Research Article Text 2022 ftpubmed https://doi.org/10.1128/spectrum.01953-21 2022-07-03T01:04:04Z Alpha mannose-oligosaccharide (MOS) prebiotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and their associated metabolism. Bio-Mos is a commercially available prebiotic currently used in the agri-feed industry, but studies show contrasting results of its effect on fish performance and feed efficiency. Thus, detailed studies are needed to investigate the effect of MOS supplements on the fish microbiome to enhance our understanding of the link between MOS and gut health. To assess Bio-Mos for potential use as a prebiotic growth promoter in salmonid aquaculture, we have modified an established Atlantic salmon in vitro gut model, SalmoSim, to evaluate its impact on the host microbial communities. The microbial communities obtained from ceca compartments from four adult farmed salmon were inoculated in biological triplicate reactors in SalmoSim. Prebiotic treatment was supplemented for 20 days, followed by a 6-day washout period. Inclusion of Bio-Mos in the media resulted in a significant increase in formate (P = 0.001), propionate (P = 0.037) and 3-methyl butanoic acid (P = 0.024) levels, correlated with increased abundances of several, principally, anaerobic microbial genera (Fusobacterium, Agarivorans, Pseudoalteromonas). DNA metabarcoding with the 16S rDNA marker confirmed a significant shift in microbial community composition in response to Bio-Mos supplementation with observed increase in lactic acid producing Carnobacterium. In conjunction with previous in vivo studies linking enhanced volatile fatty acid production alongside MOS supplementation to host growth and performance, our data suggest that Bio-Mos may be of value in salmonid production. Furthermore, our data highlights the potential role of in vitro gut models to complementin vivo trials of microbiome modulators. IMPORTANCE In this paper we report the results of the impact of a prebiotic ... Text Atlantic salmon Salmo salar PubMed Central (PMC) Microbiology Spectrum 10 3 |
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Research Article |
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Research Article Kazlauskaite, Raminta Cheaib, Bachar Humble, Joseph Heys, Chloe Ijaz, Umer Zeeshan Connelly, Stephanie Sloan, William T. Russell, Julie Martinez-Rubio, Laura Sweetman, John Kitts, Alex McGinnity, Philip Lyons, Philip Llewellyn, Martin S. Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
topic_facet |
Research Article |
description |
Alpha mannose-oligosaccharide (MOS) prebiotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and their associated metabolism. Bio-Mos is a commercially available prebiotic currently used in the agri-feed industry, but studies show contrasting results of its effect on fish performance and feed efficiency. Thus, detailed studies are needed to investigate the effect of MOS supplements on the fish microbiome to enhance our understanding of the link between MOS and gut health. To assess Bio-Mos for potential use as a prebiotic growth promoter in salmonid aquaculture, we have modified an established Atlantic salmon in vitro gut model, SalmoSim, to evaluate its impact on the host microbial communities. The microbial communities obtained from ceca compartments from four adult farmed salmon were inoculated in biological triplicate reactors in SalmoSim. Prebiotic treatment was supplemented for 20 days, followed by a 6-day washout period. Inclusion of Bio-Mos in the media resulted in a significant increase in formate (P = 0.001), propionate (P = 0.037) and 3-methyl butanoic acid (P = 0.024) levels, correlated with increased abundances of several, principally, anaerobic microbial genera (Fusobacterium, Agarivorans, Pseudoalteromonas). DNA metabarcoding with the 16S rDNA marker confirmed a significant shift in microbial community composition in response to Bio-Mos supplementation with observed increase in lactic acid producing Carnobacterium. In conjunction with previous in vivo studies linking enhanced volatile fatty acid production alongside MOS supplementation to host growth and performance, our data suggest that Bio-Mos may be of value in salmonid production. Furthermore, our data highlights the potential role of in vitro gut models to complementin vivo trials of microbiome modulators. IMPORTANCE In this paper we report the results of the impact of a prebiotic ... |
format |
Text |
author |
Kazlauskaite, Raminta Cheaib, Bachar Humble, Joseph Heys, Chloe Ijaz, Umer Zeeshan Connelly, Stephanie Sloan, William T. Russell, Julie Martinez-Rubio, Laura Sweetman, John Kitts, Alex McGinnity, Philip Lyons, Philip Llewellyn, Martin S. |
author_facet |
Kazlauskaite, Raminta Cheaib, Bachar Humble, Joseph Heys, Chloe Ijaz, Umer Zeeshan Connelly, Stephanie Sloan, William T. Russell, Julie Martinez-Rubio, Laura Sweetman, John Kitts, Alex McGinnity, Philip Lyons, Philip Llewellyn, Martin S. |
author_sort |
Kazlauskaite, Raminta |
title |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_short |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_full |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_fullStr |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_full_unstemmed |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_sort |
deploying an in vitro gut model to assay the impact of the mannan-oligosaccharide prebiotic bio-mos on the atlantic salmon (salmo salar) gut microbiome |
publisher |
American Society for Microbiology |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241627/ http://www.ncbi.nlm.nih.gov/pubmed/35532227 https://doi.org/10.1128/spectrum.01953-21 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Microbiol Spectr |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241627/ http://www.ncbi.nlm.nih.gov/pubmed/35532227 http://dx.doi.org/10.1128/spectrum.01953-21 |
op_rights |
Copyright © 2022 Kazlauskaite et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1128/spectrum.01953-21 |
container_title |
Microbiology Spectrum |
container_volume |
10 |
container_issue |
3 |
_version_ |
1766362567883620352 |