Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential of Parageobacillus thermantarcticus M1
Parageobacillus thermantarcticus strain M1 is a Gram-positive, motile, facultative anaerobic, spore forming, and thermophilic bacterium, isolated from geothermal soil of the crater of Mount Melbourne (74°22′ S, 164°40′ E) during the Italian Antarctic Expedition occurred in Austral summer 1986–1987....
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ftpubmed:oai:pubmedcentral.nih.gov:9218356 2023-05-15T14:06:26+02:00 Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential of Parageobacillus thermantarcticus M1 Yasar Yildiz, Songul Finore, Ilaria Leone, Luigi Romano, Ida Lama, Licia Kasavi, Ceyda Nicolaus, Barbara Toksoy Oner, Ebru Poli, Annarita 2022-06-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218356/ https://doi.org/10.3389/fmicb.2022.923038 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218356/ http://dx.doi.org/10.3389/fmicb.2022.923038 Copyright © 2022 Yasar Yildiz, Finore, Leone, Romano, Lama, Kasavi, Nicolaus, Toksoy Oner and Poli. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Microbiol Microbiology Text 2022 ftpubmed https://doi.org/10.3389/fmicb.2022.923038 2022-06-26T00:52:05Z Parageobacillus thermantarcticus strain M1 is a Gram-positive, motile, facultative anaerobic, spore forming, and thermophilic bacterium, isolated from geothermal soil of the crater of Mount Melbourne (74°22′ S, 164°40′ E) during the Italian Antarctic Expedition occurred in Austral summer 1986–1987. Strain M1 demonstrated great biotechnological and industrial potential owing to its ability to produce exopolysaccharides (EPSs), ethanol and thermostable extracellular enzymes, such as an xylanase and a β-xylosidase, and intracellular ones, such as xylose/glucose isomerase and protease. Furthermore, recent studies revealed its high potential in green chemistry due to its use in residual biomass transformation/valorization and as an appropriate model for microbial astrobiology studies. In the present study, using a systems-based approach, genomic analysis of P. thermantarcticus M1 was carried out to enlighten its functional characteristics. The elucidation of whole-genome organization of this thermophilic cell factory increased our understanding of biological mechanisms and pathways, by providing valuable information on the essential genes related to the biosynthesis of nucleotide sugar precursors, monosaccharide unit assembly, as well as the production of EPSs and ethanol. In addition, gene prediction and genome annotation studies identified genes encoding xylanolytic enzymes that are required for the conversion of lignocellulosic materials to high-value added molecules. Our findings pointed out the significant potential of strain M1 in various biotechnological and industrial applications considering its capacity to produce EPSs, ethanol and thermostable enzymes via the utilization of lignocellulosic waste materials. Text Antarc* Antarctic PubMed Central (PMC) Antarctic Austral Mount Melbourne ENVELOPE(164.700,164.700,-74.350,-74.350) Frontiers in Microbiology 13 |
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Microbiology |
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Microbiology Yasar Yildiz, Songul Finore, Ilaria Leone, Luigi Romano, Ida Lama, Licia Kasavi, Ceyda Nicolaus, Barbara Toksoy Oner, Ebru Poli, Annarita Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential of Parageobacillus thermantarcticus M1 |
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Microbiology |
description |
Parageobacillus thermantarcticus strain M1 is a Gram-positive, motile, facultative anaerobic, spore forming, and thermophilic bacterium, isolated from geothermal soil of the crater of Mount Melbourne (74°22′ S, 164°40′ E) during the Italian Antarctic Expedition occurred in Austral summer 1986–1987. Strain M1 demonstrated great biotechnological and industrial potential owing to its ability to produce exopolysaccharides (EPSs), ethanol and thermostable extracellular enzymes, such as an xylanase and a β-xylosidase, and intracellular ones, such as xylose/glucose isomerase and protease. Furthermore, recent studies revealed its high potential in green chemistry due to its use in residual biomass transformation/valorization and as an appropriate model for microbial astrobiology studies. In the present study, using a systems-based approach, genomic analysis of P. thermantarcticus M1 was carried out to enlighten its functional characteristics. The elucidation of whole-genome organization of this thermophilic cell factory increased our understanding of biological mechanisms and pathways, by providing valuable information on the essential genes related to the biosynthesis of nucleotide sugar precursors, monosaccharide unit assembly, as well as the production of EPSs and ethanol. In addition, gene prediction and genome annotation studies identified genes encoding xylanolytic enzymes that are required for the conversion of lignocellulosic materials to high-value added molecules. Our findings pointed out the significant potential of strain M1 in various biotechnological and industrial applications considering its capacity to produce EPSs, ethanol and thermostable enzymes via the utilization of lignocellulosic waste materials. |
format |
Text |
author |
Yasar Yildiz, Songul Finore, Ilaria Leone, Luigi Romano, Ida Lama, Licia Kasavi, Ceyda Nicolaus, Barbara Toksoy Oner, Ebru Poli, Annarita |
author_facet |
Yasar Yildiz, Songul Finore, Ilaria Leone, Luigi Romano, Ida Lama, Licia Kasavi, Ceyda Nicolaus, Barbara Toksoy Oner, Ebru Poli, Annarita |
author_sort |
Yasar Yildiz, Songul |
title |
Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential of Parageobacillus thermantarcticus M1 |
title_short |
Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential of Parageobacillus thermantarcticus M1 |
title_full |
Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential of Parageobacillus thermantarcticus M1 |
title_fullStr |
Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential of Parageobacillus thermantarcticus M1 |
title_full_unstemmed |
Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential of Parageobacillus thermantarcticus M1 |
title_sort |
genomic analysis provides new insights into biotechnological and industrial potential of parageobacillus thermantarcticus m1 |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218356/ https://doi.org/10.3389/fmicb.2022.923038 |
long_lat |
ENVELOPE(164.700,164.700,-74.350,-74.350) |
geographic |
Antarctic Austral Mount Melbourne |
geographic_facet |
Antarctic Austral Mount Melbourne |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Front Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218356/ http://dx.doi.org/10.3389/fmicb.2022.923038 |
op_rights |
Copyright © 2022 Yasar Yildiz, Finore, Leone, Romano, Lama, Kasavi, Nicolaus, Toksoy Oner and Poli. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.923038 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
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1766278140377694208 |