Metabolic Activity of Permafrost Bacteria below the Freezing Point
Metabolic activity was measured in the laboratory at temperatures between 5 and −20°C on the basis of incorporation of 14C-labeled acetate into lipids by samples of a natural population of bacteria from Siberian permafrost (permanently frozen soil). Incorporation followed a sigmoidal pattern similar...
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2000
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ftpubmed:oai:pubmedcentral.nih.gov:92138 2023-05-15T17:56:56+02:00 Metabolic Activity of Permafrost Bacteria below the Freezing Point Rivkina, E. M. Friedmann, E. I. McKay, C. P. Gilichinsky, D. A. 2000-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92138 http://www.ncbi.nlm.nih.gov/pubmed/10919774 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92138 http://www.ncbi.nlm.nih.gov/pubmed/10919774 Copyright © 2000, American Society for Microbiology Microbial Ecology Text 2000 ftpubmed 2013-08-29T09:20:45Z Metabolic activity was measured in the laboratory at temperatures between 5 and −20°C on the basis of incorporation of 14C-labeled acetate into lipids by samples of a natural population of bacteria from Siberian permafrost (permanently frozen soil). Incorporation followed a sigmoidal pattern similar to growth curves. At all temperatures, the log phase was followed, within 200 to 350 days, by a stationary phase, which was monitored until the 550th day of activity. The minimum doubling times ranged from 1 day (5°C) to 20 days (−10°C) to ca. 160 days (−20°C). The curves reached the stationary phase at different levels, depending on the incubation temperature. We suggest that the stationary phase, which is generally considered to be reached when the availability of nutrients becomes limiting, was brought on under our conditions by the formation of diffusion barriers in the thin layers of unfrozen water known to be present in permafrost soils, the thickness of which depends on temperature. Text permafrost PubMed Central (PMC) |
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PubMed Central (PMC) |
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language |
English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Rivkina, E. M. Friedmann, E. I. McKay, C. P. Gilichinsky, D. A. Metabolic Activity of Permafrost Bacteria below the Freezing Point |
topic_facet |
Microbial Ecology |
description |
Metabolic activity was measured in the laboratory at temperatures between 5 and −20°C on the basis of incorporation of 14C-labeled acetate into lipids by samples of a natural population of bacteria from Siberian permafrost (permanently frozen soil). Incorporation followed a sigmoidal pattern similar to growth curves. At all temperatures, the log phase was followed, within 200 to 350 days, by a stationary phase, which was monitored until the 550th day of activity. The minimum doubling times ranged from 1 day (5°C) to 20 days (−10°C) to ca. 160 days (−20°C). The curves reached the stationary phase at different levels, depending on the incubation temperature. We suggest that the stationary phase, which is generally considered to be reached when the availability of nutrients becomes limiting, was brought on under our conditions by the formation of diffusion barriers in the thin layers of unfrozen water known to be present in permafrost soils, the thickness of which depends on temperature. |
format |
Text |
author |
Rivkina, E. M. Friedmann, E. I. McKay, C. P. Gilichinsky, D. A. |
author_facet |
Rivkina, E. M. Friedmann, E. I. McKay, C. P. Gilichinsky, D. A. |
author_sort |
Rivkina, E. M. |
title |
Metabolic Activity of Permafrost Bacteria below the Freezing Point |
title_short |
Metabolic Activity of Permafrost Bacteria below the Freezing Point |
title_full |
Metabolic Activity of Permafrost Bacteria below the Freezing Point |
title_fullStr |
Metabolic Activity of Permafrost Bacteria below the Freezing Point |
title_full_unstemmed |
Metabolic Activity of Permafrost Bacteria below the Freezing Point |
title_sort |
metabolic activity of permafrost bacteria below the freezing point |
publisher |
American Society for Microbiology |
publishDate |
2000 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92138 http://www.ncbi.nlm.nih.gov/pubmed/10919774 |
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permafrost |
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permafrost |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92138 http://www.ncbi.nlm.nih.gov/pubmed/10919774 |
op_rights |
Copyright © 2000, American Society for Microbiology |
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