Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence

Cream colored bacteria from marine agar, strain WH24, WH77, and WH80 were isolated from the gill of the Crassostrea gigas a Pacific oyster with a filter-feeding habit that compels accompanying bacteria to demonstrate a high metabolic capacity, has proven able to colonize locations with changing circ...

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Published in:Current Microbiology
Main Authors: Pira, Hani, Risdian, Chandra, Müsken, Mathias, Schupp, Peter J., Wink, Joachim
Format: Text
Language:English
Published: Springer US 2022
Subjects:
Article
Pacific
Vasa
Crassostrea gigas
Pacific oyster
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200695/
http://www.ncbi.nlm.nih.gov/pubmed/35704100
https://doi.org/10.1007/s00284-022-02909-2
id ftpubmed:oai:pubmedcentral.nih.gov:9200695
record_format openpolar
spelling ftpubmed:oai:pubmedcentral.nih.gov:9200695 2023-05-15T15:58:11+02:00 Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence Pira, Hani Risdian, Chandra Müsken, Mathias Schupp, Peter J. Wink, Joachim 2022-06-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200695/ http://www.ncbi.nlm.nih.gov/pubmed/35704100 https://doi.org/10.1007/s00284-022-02909-2 en eng Springer US http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200695/ http://www.ncbi.nlm.nih.gov/pubmed/35704100 http://dx.doi.org/10.1007/s00284-022-02909-2 © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Curr Microbiol Article Text 2022 ftpubmed https://doi.org/10.1007/s00284-022-02909-2 2022-06-19T01:01:51Z Cream colored bacteria from marine agar, strain WH24, WH77, and WH80 were isolated from the gill of the Crassostrea gigas a Pacific oyster with a filter-feeding habit that compels accompanying bacteria to demonstrate a high metabolic capacity, has proven able to colonize locations with changing circumstances. Based on the 16S rRNA gene sequence, all strains had high similarity to Photobacterium arenosum CAU 1568(T) (99.72%). This study involved phenotypic traits, phylogenetic analysis, antimicrobial activity evaluation, genome mining, Co-cultivation experiments, and chemical studies of crude extracts using HPLC and LC-HRESIMS. Photobacterium arenosum WH24 and Zooshikella harenae WH53(T)were co-cultivated for 3 days in a rotary shaker at 160 rpm at 30 °C, and LC-MS monitored the chemical profiles of the co-cultures on the third day. The UV chromatograms of the extracts of the co-cultivation experiments show that Zooshikella harenae WH53(T) could be inhibited by strain WH24. The high virulence of Photobacterium arenosum WH24 was confirmed by genome analysis. Gene groups with high virulence potential were detected: tssA (ImpA), tssB (ImpB/vipA), tssC (ImpC/vipB), tssE, tssF (ImpG/vasA), tssG (ImpH/vasB), tssM (IcmF/vasK), tssJ (vasD), tssK (ImpJ/vasE), tssL (ImpK/vasF), clpV (tssH), vasH, hcp, lapP, plpD, and tpsB family. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00284-022-02909-2. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Vasa ENVELOPE(25.177,25.177,67.587,67.587) Current Microbiology 79 8
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Article
spellingShingle Article
Pira, Hani
Risdian, Chandra
Müsken, Mathias
Schupp, Peter J.
Wink, Joachim
Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence
topic_facet Article
description Cream colored bacteria from marine agar, strain WH24, WH77, and WH80 were isolated from the gill of the Crassostrea gigas a Pacific oyster with a filter-feeding habit that compels accompanying bacteria to demonstrate a high metabolic capacity, has proven able to colonize locations with changing circumstances. Based on the 16S rRNA gene sequence, all strains had high similarity to Photobacterium arenosum CAU 1568(T) (99.72%). This study involved phenotypic traits, phylogenetic analysis, antimicrobial activity evaluation, genome mining, Co-cultivation experiments, and chemical studies of crude extracts using HPLC and LC-HRESIMS. Photobacterium arenosum WH24 and Zooshikella harenae WH53(T)were co-cultivated for 3 days in a rotary shaker at 160 rpm at 30 °C, and LC-MS monitored the chemical profiles of the co-cultures on the third day. The UV chromatograms of the extracts of the co-cultivation experiments show that Zooshikella harenae WH53(T) could be inhibited by strain WH24. The high virulence of Photobacterium arenosum WH24 was confirmed by genome analysis. Gene groups with high virulence potential were detected: tssA (ImpA), tssB (ImpB/vipA), tssC (ImpC/vipB), tssE, tssF (ImpG/vasA), tssG (ImpH/vasB), tssM (IcmF/vasK), tssJ (vasD), tssK (ImpJ/vasE), tssL (ImpK/vasF), clpV (tssH), vasH, hcp, lapP, plpD, and tpsB family. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00284-022-02909-2.
format Text
author Pira, Hani
Risdian, Chandra
Müsken, Mathias
Schupp, Peter J.
Wink, Joachim
author_facet Pira, Hani
Risdian, Chandra
Müsken, Mathias
Schupp, Peter J.
Wink, Joachim
author_sort Pira, Hani
title Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence
title_short Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence
title_full Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence
title_fullStr Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence
title_full_unstemmed Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence
title_sort photobacterium arenosum wh24, isolated from the gill of pacific oyster crassostrea gigas from the north sea of germany: co-cultivation and prediction of virulence
publisher Springer US
publishDate 2022
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200695/
http://www.ncbi.nlm.nih.gov/pubmed/35704100
https://doi.org/10.1007/s00284-022-02909-2
long_lat ENVELOPE(25.177,25.177,67.587,67.587)
geographic Pacific
Vasa
geographic_facet Pacific
Vasa
genre Crassostrea gigas
Pacific oyster
genre_facet Crassostrea gigas
Pacific oyster
op_source Curr Microbiol
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200695/
http://www.ncbi.nlm.nih.gov/pubmed/35704100
http://dx.doi.org/10.1007/s00284-022-02909-2
op_rights © The Author(s) 2022
https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
op_rightsnorm CC-BY
op_doi https://doi.org/10.1007/s00284-022-02909-2
container_title Current Microbiology
container_volume 79
container_issue 8
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