COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products
The DNA analysis is the best approach to authenticate species in seafood products and to unveil frauds based on species substitution. In this study, a molecular strategy coupling Cytochrome Oxidase I (COI) DNA barcoding with the consolidated methodology of Restriction Fragment Length Polymorphisms (...
Published in: | Foods |
---|---|
Main Authors: | , , , , , , |
Format: | Text |
Language: | English |
Published: |
MDPI
2022
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180250/ https://doi.org/10.3390/foods11111569 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:9180250 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:9180250 2023-05-15T16:42:07+02:00 COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products Pappalardo, Anna Maria Giuga, Marta Raffa, Alessandra Nania, Marco Rossitto, Luana Calogero, Giada Santa Ferrito, Venera 2022-05-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180250/ https://doi.org/10.3390/foods11111569 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180250/ http://dx.doi.org/10.3390/foods11111569 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Foods Article Text 2022 ftpubmed https://doi.org/10.3390/foods11111569 2022-06-12T01:03:40Z The DNA analysis is the best approach to authenticate species in seafood products and to unveil frauds based on species substitution. In this study, a molecular strategy coupling Cytochrome Oxidase I (COI) DNA barcoding with the consolidated methodology of Restriction Fragment Length Polymorphisms (RFLPs), named COIBar-RFLP, was applied for searching pattern of restriction enzyme digestion, useful to discriminate seven different fish species (juveniles of Engraulis encrasicolus and Sardina pilchardus sold in Italy as “bianchetto” and Aphia minuta sold as “rossetto”; icefish Neosalanx tangkahkeii; European perch, Perca fluviatilis and the Nile Perch, Lates niloticus; striped catfish, Pangasianodon hypophthalmus). A total of 30 fresh and frozen samples were processed for DNA barcoding, analyzed against a barcode library of COI sequences retrieved from GenBank, and validated for COIBar–RFLP analysis. Cases of misdescription were detected: 3 samples labeled as “bianchetto” were substituted by N. tangkahkeii (2 samples) and A. minuta (1 sample); 3 samples labeled as “persico reale” (P. fluviatilis) were substituted by L. niloticus and P. hypophthalmus. All species were simultaneously discriminated through the restriction pattern obtained with MspI enzyme. The results highlighted that the COIBar-RFLP could be an effective tool to authenticate fish in seafood products by responding to the emerging interest in molecular identification technologies. Text Icefish PubMed Central (PMC) Foods 11 11 1569 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Pappalardo, Anna Maria Giuga, Marta Raffa, Alessandra Nania, Marco Rossitto, Luana Calogero, Giada Santa Ferrito, Venera COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products |
topic_facet |
Article |
description |
The DNA analysis is the best approach to authenticate species in seafood products and to unveil frauds based on species substitution. In this study, a molecular strategy coupling Cytochrome Oxidase I (COI) DNA barcoding with the consolidated methodology of Restriction Fragment Length Polymorphisms (RFLPs), named COIBar-RFLP, was applied for searching pattern of restriction enzyme digestion, useful to discriminate seven different fish species (juveniles of Engraulis encrasicolus and Sardina pilchardus sold in Italy as “bianchetto” and Aphia minuta sold as “rossetto”; icefish Neosalanx tangkahkeii; European perch, Perca fluviatilis and the Nile Perch, Lates niloticus; striped catfish, Pangasianodon hypophthalmus). A total of 30 fresh and frozen samples were processed for DNA barcoding, analyzed against a barcode library of COI sequences retrieved from GenBank, and validated for COIBar–RFLP analysis. Cases of misdescription were detected: 3 samples labeled as “bianchetto” were substituted by N. tangkahkeii (2 samples) and A. minuta (1 sample); 3 samples labeled as “persico reale” (P. fluviatilis) were substituted by L. niloticus and P. hypophthalmus. All species were simultaneously discriminated through the restriction pattern obtained with MspI enzyme. The results highlighted that the COIBar-RFLP could be an effective tool to authenticate fish in seafood products by responding to the emerging interest in molecular identification technologies. |
format |
Text |
author |
Pappalardo, Anna Maria Giuga, Marta Raffa, Alessandra Nania, Marco Rossitto, Luana Calogero, Giada Santa Ferrito, Venera |
author_facet |
Pappalardo, Anna Maria Giuga, Marta Raffa, Alessandra Nania, Marco Rossitto, Luana Calogero, Giada Santa Ferrito, Venera |
author_sort |
Pappalardo, Anna Maria |
title |
COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products |
title_short |
COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products |
title_full |
COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products |
title_fullStr |
COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products |
title_full_unstemmed |
COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products |
title_sort |
coibar-rflp molecular strategy discriminates species and unveils commercial frauds in fishery products |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180250/ https://doi.org/10.3390/foods11111569 |
genre |
Icefish |
genre_facet |
Icefish |
op_source |
Foods |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180250/ http://dx.doi.org/10.3390/foods11111569 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/foods11111569 |
container_title |
Foods |
container_volume |
11 |
container_issue |
11 |
container_start_page |
1569 |
_version_ |
1766032564379713536 |