Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats
BACKGROUND: Ixodid ticks are important vectors for zoonotic pathogens, with Ixodes ricinus being the most important in Europe. Rodents are hosts of immature life stages of I. ricinus ticks and are considered main reservoirs for tick-borne pathogens, e.g. Borrelia burgdorferi. The aim of this study w...
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ftpubmed:oai:pubmedcentral.nih.gov:9175456 2023-05-15T15:56:38+02:00 Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats Król, Nina Obiegala, Anna Imholt, Christian Arz, Charlotte Schmidt, Elisabeth Jeske, Kathrin Ulrich, Rainer Günter Rentería‑Solís, Zaida Jacob, Jens Pfeffer, Martin 2022-06-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175456/ https://doi.org/10.1186/s13071-022-05326-3 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175456/ http://dx.doi.org/10.1186/s13071-022-05326-3 © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. CC0 PDM CC-BY Parasit Vectors Research Text 2022 ftpubmed https://doi.org/10.1186/s13071-022-05326-3 2022-06-12T00:56:37Z BACKGROUND: Ixodid ticks are important vectors for zoonotic pathogens, with Ixodes ricinus being the most important in Europe. Rodents are hosts of immature life stages of I. ricinus ticks and are considered main reservoirs for tick-borne pathogens, e.g. Borrelia burgdorferi. The aim of this study was to analyse the prevalence as well as genospecies and sequence type (ST) diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from central Germany and to elaborate on the influence of environmental and/or individual host and vector factors on Borrelia prevalence. METHODS: After species identification, 1167 small mammal skin samples and 1094 ticks from vegetation were screened by B. burgdorferi sensu lato real-time polymerase chain reaction, and positive samples were characterized by multilocus sequence typing. Generalized linear (mixed) models were used to estimate how seasonality, small mammal species/tick life stage and habitat affect individual infection status. RESULTS: In total, 10 small mammal species and three tick species, Ixodes ricinus, Ixodes inopinatus (both considered members of the I. ricinus complex) and Dermacentor reticulatus, were investigated. Borrelia DNA was detected in eight host species, i.e. the striped field mouse (Apodemus agrarius), the yellow-necked field mouse (Apodemus flavicollis), the wood mouse (Apodemus sylvaticus), the water vole (Arvicola amphibius), the bank vole (Clethrionomys glareolus), the field vole (Microtus agrestis), the common vole (Microtus arvalis), and the common shrew (Sorex araneus). Two species were Borrelia negative, the greater white-toothed shrew (Crocidura russula) and the pygmy shrew (Sorex minutus). The average prevalence was 6.2%, with two genospecies detected, Borrelia afzelii and Borrelia garinii, and at least three STs that had not been previously reported in small mammals. Borrelia prevalence in small mammals did not differ between seasons. Six genospecies of Borrelia—Borrelia afzelii, Borrelia valaisiana, Borrelia garinii, Borrelia ... Text Common vole Microtus arvalis PubMed Central (PMC) Parasites & Vectors 15 1 |
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Research Król, Nina Obiegala, Anna Imholt, Christian Arz, Charlotte Schmidt, Elisabeth Jeske, Kathrin Ulrich, Rainer Günter Rentería‑Solís, Zaida Jacob, Jens Pfeffer, Martin Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats |
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Research |
description |
BACKGROUND: Ixodid ticks are important vectors for zoonotic pathogens, with Ixodes ricinus being the most important in Europe. Rodents are hosts of immature life stages of I. ricinus ticks and are considered main reservoirs for tick-borne pathogens, e.g. Borrelia burgdorferi. The aim of this study was to analyse the prevalence as well as genospecies and sequence type (ST) diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from central Germany and to elaborate on the influence of environmental and/or individual host and vector factors on Borrelia prevalence. METHODS: After species identification, 1167 small mammal skin samples and 1094 ticks from vegetation were screened by B. burgdorferi sensu lato real-time polymerase chain reaction, and positive samples were characterized by multilocus sequence typing. Generalized linear (mixed) models were used to estimate how seasonality, small mammal species/tick life stage and habitat affect individual infection status. RESULTS: In total, 10 small mammal species and three tick species, Ixodes ricinus, Ixodes inopinatus (both considered members of the I. ricinus complex) and Dermacentor reticulatus, were investigated. Borrelia DNA was detected in eight host species, i.e. the striped field mouse (Apodemus agrarius), the yellow-necked field mouse (Apodemus flavicollis), the wood mouse (Apodemus sylvaticus), the water vole (Arvicola amphibius), the bank vole (Clethrionomys glareolus), the field vole (Microtus agrestis), the common vole (Microtus arvalis), and the common shrew (Sorex araneus). Two species were Borrelia negative, the greater white-toothed shrew (Crocidura russula) and the pygmy shrew (Sorex minutus). The average prevalence was 6.2%, with two genospecies detected, Borrelia afzelii and Borrelia garinii, and at least three STs that had not been previously reported in small mammals. Borrelia prevalence in small mammals did not differ between seasons. Six genospecies of Borrelia—Borrelia afzelii, Borrelia valaisiana, Borrelia garinii, Borrelia ... |
format |
Text |
author |
Król, Nina Obiegala, Anna Imholt, Christian Arz, Charlotte Schmidt, Elisabeth Jeske, Kathrin Ulrich, Rainer Günter Rentería‑Solís, Zaida Jacob, Jens Pfeffer, Martin |
author_facet |
Król, Nina Obiegala, Anna Imholt, Christian Arz, Charlotte Schmidt, Elisabeth Jeske, Kathrin Ulrich, Rainer Günter Rentería‑Solís, Zaida Jacob, Jens Pfeffer, Martin |
author_sort |
Król, Nina |
title |
Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats |
title_short |
Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats |
title_full |
Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats |
title_fullStr |
Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats |
title_full_unstemmed |
Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats |
title_sort |
diversity of borrelia burgdorferi sensu lato in ticks and small mammals from different habitats |
publisher |
BioMed Central |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175456/ https://doi.org/10.1186/s13071-022-05326-3 |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_source |
Parasit Vectors |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175456/ http://dx.doi.org/10.1186/s13071-022-05326-3 |
op_rights |
© The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
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CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s13071-022-05326-3 |
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