Genetic subtyping and phylogenetic analysis of HA and NA from avian influenza virus in wild birds from Peru reveals unique features among circulating strains in America
Avian influenza virus (AIV) represents a major concern with productive implications in poultry systems but it is also a zoonotic agent that possesses an intrinsic pandemic risk. AIV is an enveloped, negative-sense and single-stranded RNA virus with a segmented genome. The eight genomic segments, com...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Public Library of Science
2022
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173603/ http://www.ncbi.nlm.nih.gov/pubmed/35671300 https://doi.org/10.1371/journal.pone.0268957 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:9173603 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:9173603 2023-05-15T18:43:27+02:00 Genetic subtyping and phylogenetic analysis of HA and NA from avian influenza virus in wild birds from Peru reveals unique features among circulating strains in America Castro-Sanguinetti, Gina R. Marques Simas, Paulo Vitor Apaza-Chiara, Ana Paola Callupe-Leyva, Jose Alonso Rondon-Espinoza, Juan Alexander Gavidia, Cesar M. More-Bayona, Juan Anderson Gonzalez Veliz, Rosa Isabel Vakharia, Vikram N. Icochea, Maria Eliana 2022-06-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173603/ http://www.ncbi.nlm.nih.gov/pubmed/35671300 https://doi.org/10.1371/journal.pone.0268957 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173603/ http://www.ncbi.nlm.nih.gov/pubmed/35671300 http://dx.doi.org/10.1371/journal.pone.0268957 © 2022 Castro-Sanguinetti et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY PLoS One Research Article Text 2022 ftpubmed https://doi.org/10.1371/journal.pone.0268957 2022-06-12T00:52:48Z Avian influenza virus (AIV) represents a major concern with productive implications in poultry systems but it is also a zoonotic agent that possesses an intrinsic pandemic risk. AIV is an enveloped, negative-sense and single-stranded RNA virus with a segmented genome. The eight genomic segments, comprising the whole genome, encode for eleven proteins. Within these proteins, Hemagglutinin (HA) and Neuraminidase (NA) are the most relevant for studies of evolution and pathogenesis considering their role in viral replication, and have also been used for classification purposes. Migratory birds are the main hosts and play a pivotal role in viral evolution and dissemination due to their migratory routes that comprise large regions worldwide. Altogether, viral and reservoir factors contribute to the emergence of avian influenza viruses with novel features and pathogenic potentials. The study aimed to conduct surveillance of AIVs in wild birds from Peru. A multi-site screening of feces of migratory birds was performed to isolate viruses and to characterize the whole genome sequences, especially the genes coding for HA and NA proteins. Four-hundred-twenty-one (421) fecal samples, collected between March 2019 and March 2020 in Lima, were obtained from 21 species of wild birds. From these, we isolated five AIV from whimbrel, kelp gull, Franklin’s gulls and Mallard, which were of low pathogenicity, including four subtypes as H6N8, H13N6, H6N2 and H2N6. Genetic analysis of HA and NA genes revealed novel features in these viruses and phylogenetic analysis exhibited a close relationship with those identified in North America (US and Canada). Furthermore, H2N6 isolate presented a NA sequence with higher genetic relationship to Chilean isolates. These results highlight that the geographical factor is of major relevance in the evolution of AIV, suggesting that AIV circulating in Peru might represent a new site for the emergence of reassortant AIVs. Text Whimbrel PubMed Central (PMC) Canada PLOS ONE 17 6 e0268957 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Castro-Sanguinetti, Gina R. Marques Simas, Paulo Vitor Apaza-Chiara, Ana Paola Callupe-Leyva, Jose Alonso Rondon-Espinoza, Juan Alexander Gavidia, Cesar M. More-Bayona, Juan Anderson Gonzalez Veliz, Rosa Isabel Vakharia, Vikram N. Icochea, Maria Eliana Genetic subtyping and phylogenetic analysis of HA and NA from avian influenza virus in wild birds from Peru reveals unique features among circulating strains in America |
topic_facet |
Research Article |
description |
Avian influenza virus (AIV) represents a major concern with productive implications in poultry systems but it is also a zoonotic agent that possesses an intrinsic pandemic risk. AIV is an enveloped, negative-sense and single-stranded RNA virus with a segmented genome. The eight genomic segments, comprising the whole genome, encode for eleven proteins. Within these proteins, Hemagglutinin (HA) and Neuraminidase (NA) are the most relevant for studies of evolution and pathogenesis considering their role in viral replication, and have also been used for classification purposes. Migratory birds are the main hosts and play a pivotal role in viral evolution and dissemination due to their migratory routes that comprise large regions worldwide. Altogether, viral and reservoir factors contribute to the emergence of avian influenza viruses with novel features and pathogenic potentials. The study aimed to conduct surveillance of AIVs in wild birds from Peru. A multi-site screening of feces of migratory birds was performed to isolate viruses and to characterize the whole genome sequences, especially the genes coding for HA and NA proteins. Four-hundred-twenty-one (421) fecal samples, collected between March 2019 and March 2020 in Lima, were obtained from 21 species of wild birds. From these, we isolated five AIV from whimbrel, kelp gull, Franklin’s gulls and Mallard, which were of low pathogenicity, including four subtypes as H6N8, H13N6, H6N2 and H2N6. Genetic analysis of HA and NA genes revealed novel features in these viruses and phylogenetic analysis exhibited a close relationship with those identified in North America (US and Canada). Furthermore, H2N6 isolate presented a NA sequence with higher genetic relationship to Chilean isolates. These results highlight that the geographical factor is of major relevance in the evolution of AIV, suggesting that AIV circulating in Peru might represent a new site for the emergence of reassortant AIVs. |
format |
Text |
author |
Castro-Sanguinetti, Gina R. Marques Simas, Paulo Vitor Apaza-Chiara, Ana Paola Callupe-Leyva, Jose Alonso Rondon-Espinoza, Juan Alexander Gavidia, Cesar M. More-Bayona, Juan Anderson Gonzalez Veliz, Rosa Isabel Vakharia, Vikram N. Icochea, Maria Eliana |
author_facet |
Castro-Sanguinetti, Gina R. Marques Simas, Paulo Vitor Apaza-Chiara, Ana Paola Callupe-Leyva, Jose Alonso Rondon-Espinoza, Juan Alexander Gavidia, Cesar M. More-Bayona, Juan Anderson Gonzalez Veliz, Rosa Isabel Vakharia, Vikram N. Icochea, Maria Eliana |
author_sort |
Castro-Sanguinetti, Gina R. |
title |
Genetic subtyping and phylogenetic analysis of HA and NA from avian influenza virus in wild birds from Peru reveals unique features among circulating strains in America |
title_short |
Genetic subtyping and phylogenetic analysis of HA and NA from avian influenza virus in wild birds from Peru reveals unique features among circulating strains in America |
title_full |
Genetic subtyping and phylogenetic analysis of HA and NA from avian influenza virus in wild birds from Peru reveals unique features among circulating strains in America |
title_fullStr |
Genetic subtyping and phylogenetic analysis of HA and NA from avian influenza virus in wild birds from Peru reveals unique features among circulating strains in America |
title_full_unstemmed |
Genetic subtyping and phylogenetic analysis of HA and NA from avian influenza virus in wild birds from Peru reveals unique features among circulating strains in America |
title_sort |
genetic subtyping and phylogenetic analysis of ha and na from avian influenza virus in wild birds from peru reveals unique features among circulating strains in america |
publisher |
Public Library of Science |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173603/ http://www.ncbi.nlm.nih.gov/pubmed/35671300 https://doi.org/10.1371/journal.pone.0268957 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Whimbrel |
genre_facet |
Whimbrel |
op_source |
PLoS One |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173603/ http://www.ncbi.nlm.nih.gov/pubmed/35671300 http://dx.doi.org/10.1371/journal.pone.0268957 |
op_rights |
© 2022 Castro-Sanguinetti et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0268957 |
container_title |
PLOS ONE |
container_volume |
17 |
container_issue |
6 |
container_start_page |
e0268957 |
_version_ |
1766233851896528896 |