Reduced H(+) channel activity disrupts pH homeostasis and calcification in coccolithophores at low ocean pH
Coccolithophores are major producers of ocean biogenic calcite, but this process is predicted to be negatively affected by future ocean acidification scenarios. Since coccolithophores calcify intracellularly, the mechanisms through which changes in seawater carbonate chemistry affect calcification r...
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ftpubmed:oai:pubmedcentral.nih.gov:9171652 2023-05-15T17:51:08+02:00 Reduced H(+) channel activity disrupts pH homeostasis and calcification in coccolithophores at low ocean pH Kottmeier, Dorothee M. Chrachri, Abdesslam Langer, Gerald Helliwell, Katherine E. Wheeler, Glen L. Brownlee, Colin 2022-05-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171652/ http://www.ncbi.nlm.nih.gov/pubmed/35522711 https://doi.org/10.1073/pnas.2118009119 en eng National Academy of Sciences http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171652/ http://www.ncbi.nlm.nih.gov/pubmed/35522711 http://dx.doi.org/10.1073/pnas.2118009119 Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . CC-BY Proc Natl Acad Sci U S A Biological Sciences Text 2022 ftpubmed https://doi.org/10.1073/pnas.2118009119 2022-06-12T00:50:34Z Coccolithophores are major producers of ocean biogenic calcite, but this process is predicted to be negatively affected by future ocean acidification scenarios. Since coccolithophores calcify intracellularly, the mechanisms through which changes in seawater carbonate chemistry affect calcification remain unclear. Here we show that voltage-gated H(+) channels in the plasma membrane of Coccolithus braarudii serve to regulate pH and maintain calcification under normal conditions but have greatly reduced activity in cells acclimated to low pH. This disrupts intracellular pH homeostasis and impairs the ability of C. braarudii to remove H(+) generated by the calcification process, leading to specific coccolith malformations. These coccolith malformations can be reproduced by pharmacological inhibition of H(+) channels. Heavily calcified coccolithophore species such as C. braarudii, which make the major contribution to carbonate export to the deep ocean, have a large intracellular H(+) load and are likely to be most vulnerable to future decreases in ocean pH. Text Ocean acidification PubMed Central (PMC) Proceedings of the National Academy of Sciences 119 19 |
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English |
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Biological Sciences |
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Biological Sciences Kottmeier, Dorothee M. Chrachri, Abdesslam Langer, Gerald Helliwell, Katherine E. Wheeler, Glen L. Brownlee, Colin Reduced H(+) channel activity disrupts pH homeostasis and calcification in coccolithophores at low ocean pH |
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Biological Sciences |
description |
Coccolithophores are major producers of ocean biogenic calcite, but this process is predicted to be negatively affected by future ocean acidification scenarios. Since coccolithophores calcify intracellularly, the mechanisms through which changes in seawater carbonate chemistry affect calcification remain unclear. Here we show that voltage-gated H(+) channels in the plasma membrane of Coccolithus braarudii serve to regulate pH and maintain calcification under normal conditions but have greatly reduced activity in cells acclimated to low pH. This disrupts intracellular pH homeostasis and impairs the ability of C. braarudii to remove H(+) generated by the calcification process, leading to specific coccolith malformations. These coccolith malformations can be reproduced by pharmacological inhibition of H(+) channels. Heavily calcified coccolithophore species such as C. braarudii, which make the major contribution to carbonate export to the deep ocean, have a large intracellular H(+) load and are likely to be most vulnerable to future decreases in ocean pH. |
format |
Text |
author |
Kottmeier, Dorothee M. Chrachri, Abdesslam Langer, Gerald Helliwell, Katherine E. Wheeler, Glen L. Brownlee, Colin |
author_facet |
Kottmeier, Dorothee M. Chrachri, Abdesslam Langer, Gerald Helliwell, Katherine E. Wheeler, Glen L. Brownlee, Colin |
author_sort |
Kottmeier, Dorothee M. |
title |
Reduced H(+) channel activity disrupts pH homeostasis and calcification in coccolithophores at low ocean pH |
title_short |
Reduced H(+) channel activity disrupts pH homeostasis and calcification in coccolithophores at low ocean pH |
title_full |
Reduced H(+) channel activity disrupts pH homeostasis and calcification in coccolithophores at low ocean pH |
title_fullStr |
Reduced H(+) channel activity disrupts pH homeostasis and calcification in coccolithophores at low ocean pH |
title_full_unstemmed |
Reduced H(+) channel activity disrupts pH homeostasis and calcification in coccolithophores at low ocean pH |
title_sort |
reduced h(+) channel activity disrupts ph homeostasis and calcification in coccolithophores at low ocean ph |
publisher |
National Academy of Sciences |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171652/ http://www.ncbi.nlm.nih.gov/pubmed/35522711 https://doi.org/10.1073/pnas.2118009119 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Proc Natl Acad Sci U S A |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171652/ http://www.ncbi.nlm.nih.gov/pubmed/35522711 http://dx.doi.org/10.1073/pnas.2118009119 |
op_rights |
Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
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CC-BY |
op_doi |
https://doi.org/10.1073/pnas.2118009119 |
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Proceedings of the National Academy of Sciences |
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119 |
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19 |
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1766158182040731648 |