Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production
Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, high production costs, low productivity and unsatisfactory scale-up production have hampered commercial adoption. Herein, we report for the first time the β-...
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Springer Berlin Heidelberg
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ftpubmed:oai:pubmedcentral.nih.gov:9159787 2023-05-15T13:36:02+02:00 Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production Nascimento, Miguel Figueiredo Barreiros, Ricardo Oliveira, Ana Cristina Ferreira, Frederico Castelo Faria, Nuno Torres 2022-06-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159787/ https://doi.org/10.1007/s13399-022-02837-y en eng Springer Berlin Heidelberg http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159787/ http://dx.doi.org/10.1007/s13399-022-02837-y © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. Biomass Convers Biorefin Original Article Text 2022 ftpubmed https://doi.org/10.1007/s13399-022-02837-y 2022-06-05T01:19:24Z Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, high production costs, low productivity and unsatisfactory scale-up production have hampered commercial adoption. Herein, we report for the first time the β-galactosidase production by Moesziomyces spp. from different sugars (D-galactose, D-glucose and D-lactose), with D-galactose being the best β-galactosidase inducer, with 11.2 and 63.1 IU/mg(biomass), for Moesziomyces aphidis 5535( T) and Moesziomyces antarcticus 5048( T), respectively. The production of this enzyme allows to break down D-lactose and thus to produce MEL directly from D-lactose or cheese whey (a cheese industry by-product). Remarkably, when CW was used as sole media component (carbon and mineral source), in combination with waste frying oil, MEL productivities were very close (1.40 and 1.31 g(MEL)/L/day) to the ones obtained with optimized medium containing yeast extract (1.92 and 1.50 g(MEL)/g(susbtrate)), both for M. antarcticus and M. aphidis. The low-cost, facile and efficient process which generates large amounts of MELs potentiates its industrialization. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13399-022-02837-y. Text Antarc* antarcticus PubMed Central (PMC) Biomass Conversion and Biorefinery |
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PubMed Central (PMC) |
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English |
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Original Article |
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Original Article Nascimento, Miguel Figueiredo Barreiros, Ricardo Oliveira, Ana Cristina Ferreira, Frederico Castelo Faria, Nuno Torres Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production |
topic_facet |
Original Article |
description |
Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, high production costs, low productivity and unsatisfactory scale-up production have hampered commercial adoption. Herein, we report for the first time the β-galactosidase production by Moesziomyces spp. from different sugars (D-galactose, D-glucose and D-lactose), with D-galactose being the best β-galactosidase inducer, with 11.2 and 63.1 IU/mg(biomass), for Moesziomyces aphidis 5535( T) and Moesziomyces antarcticus 5048( T), respectively. The production of this enzyme allows to break down D-lactose and thus to produce MEL directly from D-lactose or cheese whey (a cheese industry by-product). Remarkably, when CW was used as sole media component (carbon and mineral source), in combination with waste frying oil, MEL productivities were very close (1.40 and 1.31 g(MEL)/L/day) to the ones obtained with optimized medium containing yeast extract (1.92 and 1.50 g(MEL)/g(susbtrate)), both for M. antarcticus and M. aphidis. The low-cost, facile and efficient process which generates large amounts of MELs potentiates its industrialization. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13399-022-02837-y. |
format |
Text |
author |
Nascimento, Miguel Figueiredo Barreiros, Ricardo Oliveira, Ana Cristina Ferreira, Frederico Castelo Faria, Nuno Torres |
author_facet |
Nascimento, Miguel Figueiredo Barreiros, Ricardo Oliveira, Ana Cristina Ferreira, Frederico Castelo Faria, Nuno Torres |
author_sort |
Nascimento, Miguel Figueiredo |
title |
Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production |
title_short |
Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production |
title_full |
Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production |
title_fullStr |
Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production |
title_full_unstemmed |
Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production |
title_sort |
moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production |
publisher |
Springer Berlin Heidelberg |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159787/ https://doi.org/10.1007/s13399-022-02837-y |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
Biomass Convers Biorefin |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159787/ http://dx.doi.org/10.1007/s13399-022-02837-y |
op_rights |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
op_doi |
https://doi.org/10.1007/s13399-022-02837-y |
container_title |
Biomass Conversion and Biorefinery |
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1766073583652569088 |