Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenser baerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H(2)O(2)-Damaged Human Umbilical Vein Endothelial Cells (HUVECs)
For making full use of aquatic by-products to produce high value-added products, Siberian sturgeon (Acipenser baerii) cartilages were degreased, mineralized, and separately hydrolyzed by five kinds of proteases. The collagen hydrolysate (SCH) generated by Alcalase showed the strongest 2,2-diphenyl-1...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146044/ http://www.ncbi.nlm.nih.gov/pubmed/35621976 https://doi.org/10.3390/md20050325 |
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ftpubmed:oai:pubmedcentral.nih.gov:9146044 2023-05-15T13:01:45+02:00 Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenser baerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H(2)O(2)-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) Sheng, Yan Qiu, Yi-Ting Wang, Yu-Mei Chi, Chang-Feng Wang, Bin 2022-05-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146044/ http://www.ncbi.nlm.nih.gov/pubmed/35621976 https://doi.org/10.3390/md20050325 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146044/ http://www.ncbi.nlm.nih.gov/pubmed/35621976 http://dx.doi.org/10.3390/md20050325 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Mar Drugs Article Text 2022 ftpubmed https://doi.org/10.3390/md20050325 2022-06-05T00:58:11Z For making full use of aquatic by-products to produce high value-added products, Siberian sturgeon (Acipenser baerii) cartilages were degreased, mineralized, and separately hydrolyzed by five kinds of proteases. The collagen hydrolysate (SCH) generated by Alcalase showed the strongest 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) and hydroxide radical (HO·) scavenging activity. Subsequently, thirteen antioxidant peptides (SCP1-SCP3) were isolated from SCH, and they were identified as GPTGED, GEPGEQ, GPEGPAG, VPPQD, GLEDHA, GDRGAEG, PRGFRGPV, GEYGFE, GFIGFNG, PSVSLT, IELFPGLP, LRGEAGL, and RGEPGL with molecular weights of 574.55, 615.60, 583.60, 554.60, 640.64, 660.64, 885.04, 700.70, 710.79, 602.67, 942.12, 714.82, and 627.70 Da, respectively. GEYGFE, PSVSLT, and IELFPGLP showed the highest scavenging activity on DPPH· (EC(50): 1.27, 1.05, and 1.38 mg/mL, respectively) and HO· (EC(50): 1.16, 0.97, and 1.63 mg/mL, respectively), inhibiting capability of lipid peroxidation, and protective functions on H(2)O(2)-damaged plasmid DNA. More importantly, GEYGFE, PSVSLT, and IELFPGLP displayed significant cytoprotection on HUVECs against H(2)O(2) injury by regulating the endogenous antioxidant enzymes of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) to decrease the contents of reactive oxygen species (ROS) and malondialdehyde (MDA). Therefore, the research provided better technical assistance for a higher-value utilization of Siberian sturgeon cartilages and the thirteen isolated peptides—especially GEYGFE, PSVSLT, and IELFPGLP—which may serve as antioxidant additives for generating health-prone products to treat chronic diseases caused by oxidative stress. Text Acipenser baerii Siberian sturgeon PubMed Central (PMC) Marine Drugs 20 5 325 |
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Article Sheng, Yan Qiu, Yi-Ting Wang, Yu-Mei Chi, Chang-Feng Wang, Bin Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenser baerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H(2)O(2)-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
topic_facet |
Article |
description |
For making full use of aquatic by-products to produce high value-added products, Siberian sturgeon (Acipenser baerii) cartilages were degreased, mineralized, and separately hydrolyzed by five kinds of proteases. The collagen hydrolysate (SCH) generated by Alcalase showed the strongest 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) and hydroxide radical (HO·) scavenging activity. Subsequently, thirteen antioxidant peptides (SCP1-SCP3) were isolated from SCH, and they were identified as GPTGED, GEPGEQ, GPEGPAG, VPPQD, GLEDHA, GDRGAEG, PRGFRGPV, GEYGFE, GFIGFNG, PSVSLT, IELFPGLP, LRGEAGL, and RGEPGL with molecular weights of 574.55, 615.60, 583.60, 554.60, 640.64, 660.64, 885.04, 700.70, 710.79, 602.67, 942.12, 714.82, and 627.70 Da, respectively. GEYGFE, PSVSLT, and IELFPGLP showed the highest scavenging activity on DPPH· (EC(50): 1.27, 1.05, and 1.38 mg/mL, respectively) and HO· (EC(50): 1.16, 0.97, and 1.63 mg/mL, respectively), inhibiting capability of lipid peroxidation, and protective functions on H(2)O(2)-damaged plasmid DNA. More importantly, GEYGFE, PSVSLT, and IELFPGLP displayed significant cytoprotection on HUVECs against H(2)O(2) injury by regulating the endogenous antioxidant enzymes of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) to decrease the contents of reactive oxygen species (ROS) and malondialdehyde (MDA). Therefore, the research provided better technical assistance for a higher-value utilization of Siberian sturgeon cartilages and the thirteen isolated peptides—especially GEYGFE, PSVSLT, and IELFPGLP—which may serve as antioxidant additives for generating health-prone products to treat chronic diseases caused by oxidative stress. |
format |
Text |
author |
Sheng, Yan Qiu, Yi-Ting Wang, Yu-Mei Chi, Chang-Feng Wang, Bin |
author_facet |
Sheng, Yan Qiu, Yi-Ting Wang, Yu-Mei Chi, Chang-Feng Wang, Bin |
author_sort |
Sheng, Yan |
title |
Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenser baerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H(2)O(2)-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
title_short |
Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenser baerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H(2)O(2)-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
title_full |
Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenser baerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H(2)O(2)-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
title_fullStr |
Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenser baerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H(2)O(2)-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
title_full_unstemmed |
Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenser baerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H(2)O(2)-Damaged Human Umbilical Vein Endothelial Cells (HUVECs) |
title_sort |
novel antioxidant collagen peptides of siberian sturgeon (acipenser baerii) cartilages: the preparation, characterization, and cytoprotection of h(2)o(2)-damaged human umbilical vein endothelial cells (huvecs) |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146044/ http://www.ncbi.nlm.nih.gov/pubmed/35621976 https://doi.org/10.3390/md20050325 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146044/ http://www.ncbi.nlm.nih.gov/pubmed/35621976 http://dx.doi.org/10.3390/md20050325 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/md20050325 |
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Marine Drugs |
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20 |
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5 |
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325 |
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