Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH
Fungi’s ability to convert organic materials into bioactive products offers environmentally friendly solutions for diverse industries. In the nanotechnology field, fungi metabolites have been explored for green nanoparticle synthesis. Silver nanoparticle (AgNP) research has grown rapidly over recent...
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ftpubmed:oai:pubmedcentral.nih.gov:9144091 2023-05-15T13:36:33+02:00 Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH Alves, Mariana Fuinhas Murray, Patrick G. 2022-04-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144091/ http://www.ncbi.nlm.nih.gov/pubmed/35628695 https://doi.org/10.3390/jof8050439 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144091/ http://www.ncbi.nlm.nih.gov/pubmed/35628695 http://dx.doi.org/10.3390/jof8050439 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY J Fungi (Basel) Article Text 2022 ftpubmed https://doi.org/10.3390/jof8050439 2022-06-05T00:52:12Z Fungi’s ability to convert organic materials into bioactive products offers environmentally friendly solutions for diverse industries. In the nanotechnology field, fungi metabolites have been explored for green nanoparticle synthesis. Silver nanoparticle (AgNP) research has grown rapidly over recent years mainly due to the enhanced optical, antimicrobial and anticancer properties of AgNPs, which make them extremely useful in the biomedicine and biotechnology field. However, the biological synthesis mechanism is still not fully established. Therefore, this study aimed to evaluate the combined effect of time, temperature and pH variation in AgNP synthesis using three different fungi phyla (Ascomycota, Basidiomycota and Zygomycota) represented by six different fungi species: Cladophialophora bantiana (C. bantiana), Penicillium antarcticum (P. antarcticum), Trametes versicolor (T. versicolor), Trichoderma martiale (T. martiale), Umbelopsis isabellina (U. isabellina) and Bjerkandera adusta (B. adusta). Ultraviolet–visible (UV-Vis) spectrophotometry and transmission electron microscopy (TEM) results demonstrated the synthesis of AgNPs of different sizes (3 to 17 nm) and dispersity percentages (25 to 95%, within the same size range) using fungi extracts by changing physicochemical reaction parameters. It was observed that higher temperatures (90 °C) associated with basic pH (9 and 12) favoured the synthesis of monodisperse small AgNPs. Previous studies demonstrated enhanced antibacterial and anticancer properties correlated with smaller nanoparticle sizes. Therefore, the biologically synthesised AgNPs shown in this study have potential as sustainable substitutes for chemically made antibacterial and anticancer products. It was also shown that not all fungi species (B. adusta) secrete metabolites capable of reducing silver nitrate (AgNO(3)) precursors into AgNPs, demonstrating the importance of fungal screening studies. Text Antarc* PubMed Central (PMC) Journal of Fungi 8 5 439 |
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Article Alves, Mariana Fuinhas Murray, Patrick G. Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH |
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description |
Fungi’s ability to convert organic materials into bioactive products offers environmentally friendly solutions for diverse industries. In the nanotechnology field, fungi metabolites have been explored for green nanoparticle synthesis. Silver nanoparticle (AgNP) research has grown rapidly over recent years mainly due to the enhanced optical, antimicrobial and anticancer properties of AgNPs, which make them extremely useful in the biomedicine and biotechnology field. However, the biological synthesis mechanism is still not fully established. Therefore, this study aimed to evaluate the combined effect of time, temperature and pH variation in AgNP synthesis using three different fungi phyla (Ascomycota, Basidiomycota and Zygomycota) represented by six different fungi species: Cladophialophora bantiana (C. bantiana), Penicillium antarcticum (P. antarcticum), Trametes versicolor (T. versicolor), Trichoderma martiale (T. martiale), Umbelopsis isabellina (U. isabellina) and Bjerkandera adusta (B. adusta). Ultraviolet–visible (UV-Vis) spectrophotometry and transmission electron microscopy (TEM) results demonstrated the synthesis of AgNPs of different sizes (3 to 17 nm) and dispersity percentages (25 to 95%, within the same size range) using fungi extracts by changing physicochemical reaction parameters. It was observed that higher temperatures (90 °C) associated with basic pH (9 and 12) favoured the synthesis of monodisperse small AgNPs. Previous studies demonstrated enhanced antibacterial and anticancer properties correlated with smaller nanoparticle sizes. Therefore, the biologically synthesised AgNPs shown in this study have potential as sustainable substitutes for chemically made antibacterial and anticancer products. It was also shown that not all fungi species (B. adusta) secrete metabolites capable of reducing silver nitrate (AgNO(3)) precursors into AgNPs, demonstrating the importance of fungal screening studies. |
format |
Text |
author |
Alves, Mariana Fuinhas Murray, Patrick G. |
author_facet |
Alves, Mariana Fuinhas Murray, Patrick G. |
author_sort |
Alves, Mariana Fuinhas |
title |
Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH |
title_short |
Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH |
title_full |
Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH |
title_fullStr |
Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH |
title_full_unstemmed |
Biological Synthesis of Monodisperse Uniform-Size Silver Nanoparticles (AgNPs) by Fungal Cell-Free Extracts at Elevated Temperature and pH |
title_sort |
biological synthesis of monodisperse uniform-size silver nanoparticles (agnps) by fungal cell-free extracts at elevated temperature and ph |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144091/ http://www.ncbi.nlm.nih.gov/pubmed/35628695 https://doi.org/10.3390/jof8050439 |
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Antarc* |
genre_facet |
Antarc* |
op_source |
J Fungi (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144091/ http://www.ncbi.nlm.nih.gov/pubmed/35628695 http://dx.doi.org/10.3390/jof8050439 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/jof8050439 |
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Journal of Fungi |
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8 |
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5 |
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439 |
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