Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of...
Published in: | Communications Biology |
---|---|
Main Authors: | , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Nature Publishing Group UK
2022
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133084/ https://doi.org/10.1038/s42003-022-03461-2 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:9133084 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:9133084 2023-05-15T14:49:40+02:00 Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis Freyria, Nastasia J. Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Lovejoy, Connie 2022-05-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133084/ https://doi.org/10.1038/s42003-022-03461-2 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133084/ http://dx.doi.org/10.1038/s42003-022-03461-2 © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Commun Biol Article Text 2022 ftpubmed https://doi.org/10.1038/s42003-022-03461-2 2022-05-29T00:53:08Z Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na(+)-H(+) antiporters and Na(+) -Pi symporters as salinity decreases, but the K(+) channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. Text Arctic Sea ice PubMed Central (PMC) Arctic Communications Biology 5 1 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Freyria, Nastasia J. Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Lovejoy, Connie Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
topic_facet |
Article |
description |
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na(+)-H(+) antiporters and Na(+) -Pi symporters as salinity decreases, but the K(+) channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. |
format |
Text |
author |
Freyria, Nastasia J. Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Lovejoy, Connie |
author_facet |
Freyria, Nastasia J. Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Lovejoy, Connie |
author_sort |
Freyria, Nastasia J. |
title |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_short |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_full |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_fullStr |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_full_unstemmed |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_sort |
salinity tolerance mechanisms of an arctic pelagophyte using comparative transcriptomic and gene expression analysis |
publisher |
Nature Publishing Group UK |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133084/ https://doi.org/10.1038/s42003-022-03461-2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Sea ice |
genre_facet |
Arctic Sea ice |
op_source |
Commun Biol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133084/ http://dx.doi.org/10.1038/s42003-022-03461-2 |
op_rights |
© The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s42003-022-03461-2 |
container_title |
Communications Biology |
container_volume |
5 |
container_issue |
1 |
_version_ |
1766320750869872640 |