Biodiversity of Clostridium botulinum Type E Strains Isolated from Fish and Fishery Products
The genetic biodiversity of Clostridium botulinum type E strains was studied by pulsed-field gel electrophoresis (PFGE) with two macrorestriction enzymes (SmaI-XmaI and XhoI) and by randomly amplified polymorphic DNA (RAPD) analysis with two primers (OPJ 6 and OPJ 13) to characterize 67 Finnish isol...
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ftpubmed:oai:pubmedcentral.nih.gov:91298 2023-05-15T17:31:07+02:00 Biodiversity of Clostridium botulinum Type E Strains Isolated from Fish and Fishery Products Hyytiä, Eija Hielm, Sebastian Björkroth, Johanna Korkeala, Hannu 1999-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91298 http://www.ncbi.nlm.nih.gov/pubmed/10224001 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91298 http://www.ncbi.nlm.nih.gov/pubmed/10224001 Copyright © 1999, American Society for Microbiology Food Microbiology Text 1999 ftpubmed 2013-08-29T09:18:03Z The genetic biodiversity of Clostridium botulinum type E strains was studied by pulsed-field gel electrophoresis (PFGE) with two macrorestriction enzymes (SmaI-XmaI and XhoI) and by randomly amplified polymorphic DNA (RAPD) analysis with two primers (OPJ 6 and OPJ 13) to characterize 67 Finnish isolates from fresh fish and fishery products, 15 German isolates from farmed fish, and 10 isolates of North American or North Atlantic origin derived mainly from different types of seafood. The effects of fish species, processing, and geographical origin on the epidemiology of the isolates were evaluated. Cluster analysis based on macrorestriction profiles was performed to study the genetic relationships of the isolates. PFGE and RAPD analyses were combined and resulted in the identification of 62 different subtypes among the 92 type E isolates analyzed. High genetic biodiversity among the isolates was observed regardless of their source. Finnish and North American or North Atlantic isolates did not form distinctly discernible clusters, in contrast with the genetically homogeneous group of German isolates. On the other hand, indistinguishable or closely related genetic profiles among epidemiologically unrelated samples were detected. It was concluded that the high genetic variation was probably a result of a lack of strong selection factors that would influence the evolution of type E. The wide genetic biodiversity observed among type E isolates indicates the value of DNA-based typing methods as a tool in contamination studies in the food industry and in investigations of botulism outbreaks. Text North Atlantic PubMed Central (PMC) |
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Food Microbiology |
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Food Microbiology Hyytiä, Eija Hielm, Sebastian Björkroth, Johanna Korkeala, Hannu Biodiversity of Clostridium botulinum Type E Strains Isolated from Fish and Fishery Products |
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Food Microbiology |
description |
The genetic biodiversity of Clostridium botulinum type E strains was studied by pulsed-field gel electrophoresis (PFGE) with two macrorestriction enzymes (SmaI-XmaI and XhoI) and by randomly amplified polymorphic DNA (RAPD) analysis with two primers (OPJ 6 and OPJ 13) to characterize 67 Finnish isolates from fresh fish and fishery products, 15 German isolates from farmed fish, and 10 isolates of North American or North Atlantic origin derived mainly from different types of seafood. The effects of fish species, processing, and geographical origin on the epidemiology of the isolates were evaluated. Cluster analysis based on macrorestriction profiles was performed to study the genetic relationships of the isolates. PFGE and RAPD analyses were combined and resulted in the identification of 62 different subtypes among the 92 type E isolates analyzed. High genetic biodiversity among the isolates was observed regardless of their source. Finnish and North American or North Atlantic isolates did not form distinctly discernible clusters, in contrast with the genetically homogeneous group of German isolates. On the other hand, indistinguishable or closely related genetic profiles among epidemiologically unrelated samples were detected. It was concluded that the high genetic variation was probably a result of a lack of strong selection factors that would influence the evolution of type E. The wide genetic biodiversity observed among type E isolates indicates the value of DNA-based typing methods as a tool in contamination studies in the food industry and in investigations of botulism outbreaks. |
format |
Text |
author |
Hyytiä, Eija Hielm, Sebastian Björkroth, Johanna Korkeala, Hannu |
author_facet |
Hyytiä, Eija Hielm, Sebastian Björkroth, Johanna Korkeala, Hannu |
author_sort |
Hyytiä, Eija |
title |
Biodiversity of Clostridium botulinum Type E Strains Isolated from Fish and Fishery Products |
title_short |
Biodiversity of Clostridium botulinum Type E Strains Isolated from Fish and Fishery Products |
title_full |
Biodiversity of Clostridium botulinum Type E Strains Isolated from Fish and Fishery Products |
title_fullStr |
Biodiversity of Clostridium botulinum Type E Strains Isolated from Fish and Fishery Products |
title_full_unstemmed |
Biodiversity of Clostridium botulinum Type E Strains Isolated from Fish and Fishery Products |
title_sort |
biodiversity of clostridium botulinum type e strains isolated from fish and fishery products |
publisher |
American Society for Microbiology |
publishDate |
1999 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91298 http://www.ncbi.nlm.nih.gov/pubmed/10224001 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91298 http://www.ncbi.nlm.nih.gov/pubmed/10224001 |
op_rights |
Copyright © 1999, American Society for Microbiology |
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