Diversity of the MHC class II DRB gene in the wolverine (Carnivora: Mustelidae: Gulo gulo) in Finland
The wolverine (Gulo gulo) in Finland has undergone significant population declines in the past. Since major histocompatibility complex (MHC) genes encode proteins involved in pathogen recognition, the diversity of these genes provides insights into the immunological fitness of regional populations....
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ftpubmed:oai:pubmedcentral.nih.gov:9089919 2023-05-15T16:32:17+02:00 Diversity of the MHC class II DRB gene in the wolverine (Carnivora: Mustelidae: Gulo gulo) in Finland Sugiyama, Yuri Nishita, Yoshinori Lansink, Gerhardus M. J. Holmala, Katja Aspi, Jouni Masuda, Ryuichi 2022-05-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089919/ http://www.ncbi.nlm.nih.gov/pubmed/35536786 https://doi.org/10.1371/journal.pone.0267609 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089919/ http://www.ncbi.nlm.nih.gov/pubmed/35536786 http://dx.doi.org/10.1371/journal.pone.0267609 © 2022 Sugiyama et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY PLoS One Research Article Text 2022 ftpubmed https://doi.org/10.1371/journal.pone.0267609 2022-05-15T01:02:31Z The wolverine (Gulo gulo) in Finland has undergone significant population declines in the past. Since major histocompatibility complex (MHC) genes encode proteins involved in pathogen recognition, the diversity of these genes provides insights into the immunological fitness of regional populations. We sequenced 862 amplicons (242 bp) of MHC class II DRB exon 2 from 32 Finnish wolverines and identified 11 functional alleles and three pseudogenes. A molecular phylogenetic analysis indicated trans-species polymorphism, and PAML and MEME analyses indicated positive selection, suggesting that the Finnish wolverine DRB genes have evolved under balancing and positive selection. In contrast to DRB gene analyses in other species, allele frequencies in the Finnish wolverines clearly indicated the existence of two regional subpopulations, congruent with previous studies based on neutral genetic markers. In the Finnish wolverine, rapid population declines in the past have promoted genetic drift, resulting in a lower genetic diversity of DRB loci, including fewer alleles and positively selected sites, than other mustelid species analyzed previously. Our data suggest that the MHC region in the Finnish wolverine population was likely affected by a recent bottleneck. Text Gulo gulo PubMed Central (PMC) PLOS ONE 17 5 e0267609 |
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Research Article Sugiyama, Yuri Nishita, Yoshinori Lansink, Gerhardus M. J. Holmala, Katja Aspi, Jouni Masuda, Ryuichi Diversity of the MHC class II DRB gene in the wolverine (Carnivora: Mustelidae: Gulo gulo) in Finland |
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Research Article |
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The wolverine (Gulo gulo) in Finland has undergone significant population declines in the past. Since major histocompatibility complex (MHC) genes encode proteins involved in pathogen recognition, the diversity of these genes provides insights into the immunological fitness of regional populations. We sequenced 862 amplicons (242 bp) of MHC class II DRB exon 2 from 32 Finnish wolverines and identified 11 functional alleles and three pseudogenes. A molecular phylogenetic analysis indicated trans-species polymorphism, and PAML and MEME analyses indicated positive selection, suggesting that the Finnish wolverine DRB genes have evolved under balancing and positive selection. In contrast to DRB gene analyses in other species, allele frequencies in the Finnish wolverines clearly indicated the existence of two regional subpopulations, congruent with previous studies based on neutral genetic markers. In the Finnish wolverine, rapid population declines in the past have promoted genetic drift, resulting in a lower genetic diversity of DRB loci, including fewer alleles and positively selected sites, than other mustelid species analyzed previously. Our data suggest that the MHC region in the Finnish wolverine population was likely affected by a recent bottleneck. |
format |
Text |
author |
Sugiyama, Yuri Nishita, Yoshinori Lansink, Gerhardus M. J. Holmala, Katja Aspi, Jouni Masuda, Ryuichi |
author_facet |
Sugiyama, Yuri Nishita, Yoshinori Lansink, Gerhardus M. J. Holmala, Katja Aspi, Jouni Masuda, Ryuichi |
author_sort |
Sugiyama, Yuri |
title |
Diversity of the MHC class II DRB gene in the wolverine (Carnivora: Mustelidae: Gulo gulo) in Finland |
title_short |
Diversity of the MHC class II DRB gene in the wolverine (Carnivora: Mustelidae: Gulo gulo) in Finland |
title_full |
Diversity of the MHC class II DRB gene in the wolverine (Carnivora: Mustelidae: Gulo gulo) in Finland |
title_fullStr |
Diversity of the MHC class II DRB gene in the wolverine (Carnivora: Mustelidae: Gulo gulo) in Finland |
title_full_unstemmed |
Diversity of the MHC class II DRB gene in the wolverine (Carnivora: Mustelidae: Gulo gulo) in Finland |
title_sort |
diversity of the mhc class ii drb gene in the wolverine (carnivora: mustelidae: gulo gulo) in finland |
publisher |
Public Library of Science |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089919/ http://www.ncbi.nlm.nih.gov/pubmed/35536786 https://doi.org/10.1371/journal.pone.0267609 |
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Gulo gulo |
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Gulo gulo |
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PLoS One |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089919/ http://www.ncbi.nlm.nih.gov/pubmed/35536786 http://dx.doi.org/10.1371/journal.pone.0267609 |
op_rights |
© 2022 Sugiyama et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0267609 |
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PLOS ONE |
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17 |
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5 |
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e0267609 |
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