Green and efficient biosynthesis of indigo from indole by engineered myoglobins
With the demand nowadays for blue dyes, it is of practical importance to develop a green and efficient biocatalyst for the production of indigo. The design of artificial enzymes has been shown to be attractive in recent years. In a previous study, we engineered a single mutant of sperm whale myoglob...
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ftpubmed:oai:pubmedcentral.nih.gov:9086478 2023-05-15T18:26:48+02:00 Green and efficient biosynthesis of indigo from indole by engineered myoglobins Liu, Can Xu, Jiakun Gao, Shu-Qin He, Bo Wei, Chuan-Wan Wang, Xiao-Juan Wang, Zhonghua Lin, Ying-Wu 2018-09-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086478/ https://doi.org/10.1039/c8ra07825d en eng The Royal Society of Chemistry http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086478/ http://dx.doi.org/10.1039/c8ra07825d This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ CC-BY-NC RSC Adv Chemistry Text 2018 ftpubmed https://doi.org/10.1039/c8ra07825d 2022-05-15T00:55:29Z With the demand nowadays for blue dyes, it is of practical importance to develop a green and efficient biocatalyst for the production of indigo. The design of artificial enzymes has been shown to be attractive in recent years. In a previous study, we engineered a single mutant of sperm whale myoglobin, F43Y Mb, with a novel Tyr-heme cross-link. In this study, we found that it can efficiently catalyze the oxidation of indole to indigo, with a yield as high as 54% compared to the highest yield (∼20%) reported to date in the literature. By further modifying the heme active site, we engineered a double mutant of F43Y/H64D Mb, which exhibited the highest catalytic efficiency (198 M(−1) s(−1)) among the artificial enzymes designed in Mb. Moreover, both F43Y Mb and F43Y/H64D Mb were found to produce the indigo product with a chemoselectivity as high as ∼80%. Based on the reaction system, we also established a convenient and green dyeing method by dyeing a cotton textile during the biosynthesis of indigo, followed by further spraying the concentrated indigo, without the need of strong acids/bases or any reducing agents. The successful application of dyeing a white cotton textile with a blue color further indicates that the designed enzyme and the dyeing method have practical applications in the future. Text Sperm whale PubMed Central (PMC) RSC Advances 8 58 33325 33330 |
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Chemistry Liu, Can Xu, Jiakun Gao, Shu-Qin He, Bo Wei, Chuan-Wan Wang, Xiao-Juan Wang, Zhonghua Lin, Ying-Wu Green and efficient biosynthesis of indigo from indole by engineered myoglobins |
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Chemistry |
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With the demand nowadays for blue dyes, it is of practical importance to develop a green and efficient biocatalyst for the production of indigo. The design of artificial enzymes has been shown to be attractive in recent years. In a previous study, we engineered a single mutant of sperm whale myoglobin, F43Y Mb, with a novel Tyr-heme cross-link. In this study, we found that it can efficiently catalyze the oxidation of indole to indigo, with a yield as high as 54% compared to the highest yield (∼20%) reported to date in the literature. By further modifying the heme active site, we engineered a double mutant of F43Y/H64D Mb, which exhibited the highest catalytic efficiency (198 M(−1) s(−1)) among the artificial enzymes designed in Mb. Moreover, both F43Y Mb and F43Y/H64D Mb were found to produce the indigo product with a chemoselectivity as high as ∼80%. Based on the reaction system, we also established a convenient and green dyeing method by dyeing a cotton textile during the biosynthesis of indigo, followed by further spraying the concentrated indigo, without the need of strong acids/bases or any reducing agents. The successful application of dyeing a white cotton textile with a blue color further indicates that the designed enzyme and the dyeing method have practical applications in the future. |
format |
Text |
author |
Liu, Can Xu, Jiakun Gao, Shu-Qin He, Bo Wei, Chuan-Wan Wang, Xiao-Juan Wang, Zhonghua Lin, Ying-Wu |
author_facet |
Liu, Can Xu, Jiakun Gao, Shu-Qin He, Bo Wei, Chuan-Wan Wang, Xiao-Juan Wang, Zhonghua Lin, Ying-Wu |
author_sort |
Liu, Can |
title |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins |
title_short |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins |
title_full |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins |
title_fullStr |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins |
title_full_unstemmed |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins |
title_sort |
green and efficient biosynthesis of indigo from indole by engineered myoglobins |
publisher |
The Royal Society of Chemistry |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086478/ https://doi.org/10.1039/c8ra07825d |
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Sperm whale |
genre_facet |
Sperm whale |
op_source |
RSC Adv |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086478/ http://dx.doi.org/10.1039/c8ra07825d |
op_rights |
This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1039/c8ra07825d |
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RSC Advances |
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8 |
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58 |
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33325 |
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33330 |
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1766208774686638080 |