Enhanced catalytic activity and thermal stability of lipase bound to oxide nanosheets
The present study reports the effects of binding of lipase, which is an inexpensive digestive enzyme (candida antarctica lipase) that catalyzes the hydrolysis reaction and is frequently utilized for artificial synthesis of a variety of organic molecules, to titanate nanosheets (TNSs) on their biocat...
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ftpubmed:oai:pubmedcentral.nih.gov:9080826 2023-05-15T13:36:28+02:00 Enhanced catalytic activity and thermal stability of lipase bound to oxide nanosheets Yamada, Akane Kamada, Kai Ueda, Taro Hyodo, Takeo Shimizu, Yasuhiro Soh, Nobuaki 2018-06-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080826/ http://www.ncbi.nlm.nih.gov/pubmed/35541646 https://doi.org/10.1039/c8ra03558j en eng The Royal Society of Chemistry http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080826/ http://www.ncbi.nlm.nih.gov/pubmed/35541646 http://dx.doi.org/10.1039/c8ra03558j This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ CC-BY-NC RSC Adv Chemistry Text 2018 ftpubmed https://doi.org/10.1039/c8ra03558j 2022-05-15T00:44:38Z The present study reports the effects of binding of lipase, which is an inexpensive digestive enzyme (candida antarctica lipase) that catalyzes the hydrolysis reaction and is frequently utilized for artificial synthesis of a variety of organic molecules, to titanate nanosheets (TNSs) on their biocatalytic activities and stabilities under several lipase concentrations. TNSs were prepared through a hydrolysis reaction of titanium tetraisopropoxide (TTIP) with tetrabutylammonium hydroxide (TBAOH), resulting in formation of a colorless and transparent colloidal solution including TNSs with nanometric dimensions (hydrodynamic diameter: ca. 5.6 nm). TNSs were bound to lipase molecules through electrostatic interaction in an aqueous phase at an appropriate pH, forming inorganic-bio nanohybrids (lipase–TNSs). The enzymatic reaction rate for hydrolysis of p-nitrophenyl acetate (pNPA) catalyzed by the lipase–TNSs, especially in diluted lipase concentrations, was significantly improved more than 8 times as compared with free lipase. On the other hand, it was confirmed that heat tolerance of lipase was also improved by binding to TNSs. These results suggest that the novel lipase–TNSs proposed here have combined enhancements of the catalytic activity and the anti-denaturation stability of lipase. Text Antarc* Antarctica PubMed Central (PMC) RSC Advances 8 36 20347 20352 |
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Chemistry |
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Chemistry Yamada, Akane Kamada, Kai Ueda, Taro Hyodo, Takeo Shimizu, Yasuhiro Soh, Nobuaki Enhanced catalytic activity and thermal stability of lipase bound to oxide nanosheets |
topic_facet |
Chemistry |
description |
The present study reports the effects of binding of lipase, which is an inexpensive digestive enzyme (candida antarctica lipase) that catalyzes the hydrolysis reaction and is frequently utilized for artificial synthesis of a variety of organic molecules, to titanate nanosheets (TNSs) on their biocatalytic activities and stabilities under several lipase concentrations. TNSs were prepared through a hydrolysis reaction of titanium tetraisopropoxide (TTIP) with tetrabutylammonium hydroxide (TBAOH), resulting in formation of a colorless and transparent colloidal solution including TNSs with nanometric dimensions (hydrodynamic diameter: ca. 5.6 nm). TNSs were bound to lipase molecules through electrostatic interaction in an aqueous phase at an appropriate pH, forming inorganic-bio nanohybrids (lipase–TNSs). The enzymatic reaction rate for hydrolysis of p-nitrophenyl acetate (pNPA) catalyzed by the lipase–TNSs, especially in diluted lipase concentrations, was significantly improved more than 8 times as compared with free lipase. On the other hand, it was confirmed that heat tolerance of lipase was also improved by binding to TNSs. These results suggest that the novel lipase–TNSs proposed here have combined enhancements of the catalytic activity and the anti-denaturation stability of lipase. |
format |
Text |
author |
Yamada, Akane Kamada, Kai Ueda, Taro Hyodo, Takeo Shimizu, Yasuhiro Soh, Nobuaki |
author_facet |
Yamada, Akane Kamada, Kai Ueda, Taro Hyodo, Takeo Shimizu, Yasuhiro Soh, Nobuaki |
author_sort |
Yamada, Akane |
title |
Enhanced catalytic activity and thermal stability of lipase bound to oxide nanosheets |
title_short |
Enhanced catalytic activity and thermal stability of lipase bound to oxide nanosheets |
title_full |
Enhanced catalytic activity and thermal stability of lipase bound to oxide nanosheets |
title_fullStr |
Enhanced catalytic activity and thermal stability of lipase bound to oxide nanosheets |
title_full_unstemmed |
Enhanced catalytic activity and thermal stability of lipase bound to oxide nanosheets |
title_sort |
enhanced catalytic activity and thermal stability of lipase bound to oxide nanosheets |
publisher |
The Royal Society of Chemistry |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080826/ http://www.ncbi.nlm.nih.gov/pubmed/35541646 https://doi.org/10.1039/c8ra03558j |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
RSC Adv |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080826/ http://www.ncbi.nlm.nih.gov/pubmed/35541646 http://dx.doi.org/10.1039/c8ra03558j |
op_rights |
This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
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CC-BY-NC |
op_doi |
https://doi.org/10.1039/c8ra03558j |
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RSC Advances |
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8 |
container_issue |
36 |
container_start_page |
20347 |
op_container_end_page |
20352 |
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1766079132069789696 |