High mobility group A3 enhances transcription of the DNA demethylase gene SlDML2 to promote tomato fruit ripening
DNA methylation plays an important role in regulating tomato (Solanum lycopersicum) fruit ripening. Although SlDML2, a DNA demethylase (DML) gene, is critically involved in tomato fruit ripening, little is known about genes that regulate its expression. Using yeast one-hybrid screening, we identifie...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070846/ http://www.ncbi.nlm.nih.gov/pubmed/35171288 https://doi.org/10.1093/plphys/kiac063 |
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ftpubmed:oai:pubmedcentral.nih.gov:9070846 2023-05-15T16:01:54+02:00 High mobility group A3 enhances transcription of the DNA demethylase gene SlDML2 to promote tomato fruit ripening Li, Zhifei Pi, Ying Fan, Junmiao Yang, Xinxin Zhai, Changsheng Chen, Hong Wang, Feng Ding, Jing Gu, Tingting Li, Yi Wu, Han 2022-02-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070846/ http://www.ncbi.nlm.nih.gov/pubmed/35171288 https://doi.org/10.1093/plphys/kiac063 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070846/ http://www.ncbi.nlm.nih.gov/pubmed/35171288 http://dx.doi.org/10.1093/plphys/kiac063 © American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_modelThis article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Plant Physiol Research Articles Text 2022 ftpubmed https://doi.org/10.1093/plphys/kiac063 2023-02-19T01:32:32Z DNA methylation plays an important role in regulating tomato (Solanum lycopersicum) fruit ripening. Although SlDML2, a DNA demethylase (DML) gene, is critically involved in tomato fruit ripening, little is known about genes that regulate its expression. Using yeast one-hybrid screening, we identified a High Mobility Group A protein, named SlHMGA3, and demonstrated its binding activity to the AT-rich region of the SlDML2 promoter. We produced slhmga3 tomato mutants using CRISPR/Cas9 and observed that slhmga3 fruit reached the breaker stage much later than fruit from the wild-type. We further demonstrated that at the initiation stage of fruit ripening, the increased expression of SlDML2 and ethylene biosynthetic and signaling genes was significantly delayed in slhmga3 fruit, along with delays in ethylene production and demethylation and activation of ripening-associated transcription factor genes. Our results demonstrate that SlHMGA3 plays a role in enhancing SlDML2 expression, and its effects on tomato fruit ripening are largely through DNA demethylation of ripening-associated transcription factor genes. Text DML PubMed Central (PMC) Breaker ENVELOPE(-67.257,-67.257,-67.874,-67.874) Plant Physiology 189 1 315 328 |
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Research Articles Li, Zhifei Pi, Ying Fan, Junmiao Yang, Xinxin Zhai, Changsheng Chen, Hong Wang, Feng Ding, Jing Gu, Tingting Li, Yi Wu, Han High mobility group A3 enhances transcription of the DNA demethylase gene SlDML2 to promote tomato fruit ripening |
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Research Articles |
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DNA methylation plays an important role in regulating tomato (Solanum lycopersicum) fruit ripening. Although SlDML2, a DNA demethylase (DML) gene, is critically involved in tomato fruit ripening, little is known about genes that regulate its expression. Using yeast one-hybrid screening, we identified a High Mobility Group A protein, named SlHMGA3, and demonstrated its binding activity to the AT-rich region of the SlDML2 promoter. We produced slhmga3 tomato mutants using CRISPR/Cas9 and observed that slhmga3 fruit reached the breaker stage much later than fruit from the wild-type. We further demonstrated that at the initiation stage of fruit ripening, the increased expression of SlDML2 and ethylene biosynthetic and signaling genes was significantly delayed in slhmga3 fruit, along with delays in ethylene production and demethylation and activation of ripening-associated transcription factor genes. Our results demonstrate that SlHMGA3 plays a role in enhancing SlDML2 expression, and its effects on tomato fruit ripening are largely through DNA demethylation of ripening-associated transcription factor genes. |
format |
Text |
author |
Li, Zhifei Pi, Ying Fan, Junmiao Yang, Xinxin Zhai, Changsheng Chen, Hong Wang, Feng Ding, Jing Gu, Tingting Li, Yi Wu, Han |
author_facet |
Li, Zhifei Pi, Ying Fan, Junmiao Yang, Xinxin Zhai, Changsheng Chen, Hong Wang, Feng Ding, Jing Gu, Tingting Li, Yi Wu, Han |
author_sort |
Li, Zhifei |
title |
High mobility group A3 enhances transcription of the DNA demethylase gene SlDML2 to promote tomato fruit ripening |
title_short |
High mobility group A3 enhances transcription of the DNA demethylase gene SlDML2 to promote tomato fruit ripening |
title_full |
High mobility group A3 enhances transcription of the DNA demethylase gene SlDML2 to promote tomato fruit ripening |
title_fullStr |
High mobility group A3 enhances transcription of the DNA demethylase gene SlDML2 to promote tomato fruit ripening |
title_full_unstemmed |
High mobility group A3 enhances transcription of the DNA demethylase gene SlDML2 to promote tomato fruit ripening |
title_sort |
high mobility group a3 enhances transcription of the dna demethylase gene sldml2 to promote tomato fruit ripening |
publisher |
Oxford University Press |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070846/ http://www.ncbi.nlm.nih.gov/pubmed/35171288 https://doi.org/10.1093/plphys/kiac063 |
long_lat |
ENVELOPE(-67.257,-67.257,-67.874,-67.874) |
geographic |
Breaker |
geographic_facet |
Breaker |
genre |
DML |
genre_facet |
DML |
op_source |
Plant Physiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070846/ http://www.ncbi.nlm.nih.gov/pubmed/35171288 http://dx.doi.org/10.1093/plphys/kiac063 |
op_rights |
© American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_modelThis article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) |
op_doi |
https://doi.org/10.1093/plphys/kiac063 |
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Plant Physiology |
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189 |
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1 |
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315 |
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328 |
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