Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection
Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostre...
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ftpubmed:oai:pubmedcentral.nih.gov:9014014 2023-05-15T15:58:17+02:00 Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection Picot, Sandy Faury, Nicole Pelletier, Camille Arzul, Isabelle Chollet, Bruno Dégremont, Lionel Renault, Tristan Morga, Benjamin 2022-04-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014014/ http://www.ncbi.nlm.nih.gov/pubmed/35444958 https://doi.org/10.3389/fcimb.2022.858311 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014014/ http://www.ncbi.nlm.nih.gov/pubmed/35444958 http://dx.doi.org/10.3389/fcimb.2022.858311 Copyright © 2022 Picot, Faury, Pelletier, Arzul, Chollet, Dégremont, Renault and Morga https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Cell Infect Microbiol Cellular and Infection Microbiology Text 2022 ftpubmed https://doi.org/10.3389/fcimb.2022.858311 2022-04-24T00:37:14Z Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostreid herpesvirus 1 (OsHV-1). Autophagy is an important degradation pathway involved in the response to several pathologies including viral diseases. In C. gigas, recent studies indicate that this pathway is conserved and functional in at least haemocytes and the mantle. Furthermore, an experimental infection in combination with compounds known to inhibit or induce autophagy in mammals revealed that autophagy is involved in the response to OsHV-1 infection. In light of these results, the aim of this study was to determine the role of autophagy in the response of the Pacific oyster to infection by virus OsHV-1. For this purpose, an experimental infection in combination with a modulator of autophagy was performed on Pacific oysters known to have intermediate susceptibility to OsHV-1 infection. In haemolymph and the mantle, the autophagy response was monitored by flow cytometry, western blotting, and real-time PCR. At the same time, viral infection was evaluated by quantifying viral DNA and RNA amounts by real-time PCR. Although the results showed activation of autophagy in haemolymph and the mantle 14 hours post infection (after viral replication was initiated), they were also indicative of different regulatory mechanisms of autophagy in the two tissues, thus supporting an important function of autophagy in the response to virus OsHV-1. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Frontiers in Cellular and Infection Microbiology 12 |
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Cellular and Infection Microbiology |
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Cellular and Infection Microbiology Picot, Sandy Faury, Nicole Pelletier, Camille Arzul, Isabelle Chollet, Bruno Dégremont, Lionel Renault, Tristan Morga, Benjamin Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
topic_facet |
Cellular and Infection Microbiology |
description |
Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously affected the oyster-farming economy in several countries around the world. Although the causes of these mortality outbreaks appear complex, a viral agent has been identified as the main factor: a herpesvirus called ostreid herpesvirus 1 (OsHV-1). Autophagy is an important degradation pathway involved in the response to several pathologies including viral diseases. In C. gigas, recent studies indicate that this pathway is conserved and functional in at least haemocytes and the mantle. Furthermore, an experimental infection in combination with compounds known to inhibit or induce autophagy in mammals revealed that autophagy is involved in the response to OsHV-1 infection. In light of these results, the aim of this study was to determine the role of autophagy in the response of the Pacific oyster to infection by virus OsHV-1. For this purpose, an experimental infection in combination with a modulator of autophagy was performed on Pacific oysters known to have intermediate susceptibility to OsHV-1 infection. In haemolymph and the mantle, the autophagy response was monitored by flow cytometry, western blotting, and real-time PCR. At the same time, viral infection was evaluated by quantifying viral DNA and RNA amounts by real-time PCR. Although the results showed activation of autophagy in haemolymph and the mantle 14 hours post infection (after viral replication was initiated), they were also indicative of different regulatory mechanisms of autophagy in the two tissues, thus supporting an important function of autophagy in the response to virus OsHV-1. |
format |
Text |
author |
Picot, Sandy Faury, Nicole Pelletier, Camille Arzul, Isabelle Chollet, Bruno Dégremont, Lionel Renault, Tristan Morga, Benjamin |
author_facet |
Picot, Sandy Faury, Nicole Pelletier, Camille Arzul, Isabelle Chollet, Bruno Dégremont, Lionel Renault, Tristan Morga, Benjamin |
author_sort |
Picot, Sandy |
title |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_short |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_full |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_fullStr |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_full_unstemmed |
Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection |
title_sort |
monitoring autophagy at cellular and molecular level in crassostrea gigas during an experimental ostreid herpesvirus 1 (oshv-1) infection |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014014/ http://www.ncbi.nlm.nih.gov/pubmed/35444958 https://doi.org/10.3389/fcimb.2022.858311 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Front Cell Infect Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014014/ http://www.ncbi.nlm.nih.gov/pubmed/35444958 http://dx.doi.org/10.3389/fcimb.2022.858311 |
op_rights |
Copyright © 2022 Picot, Faury, Pelletier, Arzul, Chollet, Dégremont, Renault and Morga https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fcimb.2022.858311 |
container_title |
Frontiers in Cellular and Infection Microbiology |
container_volume |
12 |
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1766394004022231040 |