A Multistep DNA-Based Methodology for Accurate Authentication of Sturgeon Species
The sturgeons (order Acipenseriformes) are caviar producers and some of the most valuable fish species worldwide. Due to different reasons, wild populations are now at the brink of extinction. The high demand for caviar has led to the development of aquaculture for restocking and caviar production....
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ftpubmed:oai:pubmedcentral.nih.gov:8998085 2023-05-15T13:01:50+02:00 A Multistep DNA-Based Methodology for Accurate Authentication of Sturgeon Species Dudu, Andreea Samu, Maria Maereanu, Marilena Georgescu, Sergiu Emil 2022-03-29 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998085/ http://www.ncbi.nlm.nih.gov/pubmed/35407094 https://doi.org/10.3390/foods11071007 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998085/ http://www.ncbi.nlm.nih.gov/pubmed/35407094 http://dx.doi.org/10.3390/foods11071007 © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Foods Article Text 2022 ftpubmed https://doi.org/10.3390/foods11071007 2022-04-17T00:57:37Z The sturgeons (order Acipenseriformes) are caviar producers and some of the most valuable fish species worldwide. Due to different reasons, wild populations are now at the brink of extinction. The high demand for caviar has led to the development of aquaculture for restocking and caviar production. Since the caviar from different species has different prices depending on the quality and attempts of commercial fraud based on species substitution have been found, correct species identification is more than necessary. We report a new multistep methodology for an accurate species identification based on both nuclear and mitochondrial markers. Our test integrates data from the analysis of microsatellites (Afu19, Afu34, Afu39, Afu54, Aox27, AoxD234, AnacC11 and AnacE4), nuclear gene markers (RPS7, vimentin and rhodopsin) and mtDNA barcoding to give a reliable molecular diagnostic for five sturgeon species (Huso huso, Acipenser stellatus, Acipenser ruthenus, Acipenser gueldenstaedtii and Acipenser baerii). In addition to species identification, our methodology allows the identification of bester, sterbe and best beluga hybrids, but also the identification of hybrids of unknown origin. This methodology has a good potential to contribute to the conservation of highly threatened sturgeon populations and also to the traceability of their products. Text Acipenser baerii Beluga Beluga* PubMed Central (PMC) Foods 11 7 1007 |
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Article Dudu, Andreea Samu, Maria Maereanu, Marilena Georgescu, Sergiu Emil A Multistep DNA-Based Methodology for Accurate Authentication of Sturgeon Species |
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The sturgeons (order Acipenseriformes) are caviar producers and some of the most valuable fish species worldwide. Due to different reasons, wild populations are now at the brink of extinction. The high demand for caviar has led to the development of aquaculture for restocking and caviar production. Since the caviar from different species has different prices depending on the quality and attempts of commercial fraud based on species substitution have been found, correct species identification is more than necessary. We report a new multistep methodology for an accurate species identification based on both nuclear and mitochondrial markers. Our test integrates data from the analysis of microsatellites (Afu19, Afu34, Afu39, Afu54, Aox27, AoxD234, AnacC11 and AnacE4), nuclear gene markers (RPS7, vimentin and rhodopsin) and mtDNA barcoding to give a reliable molecular diagnostic for five sturgeon species (Huso huso, Acipenser stellatus, Acipenser ruthenus, Acipenser gueldenstaedtii and Acipenser baerii). In addition to species identification, our methodology allows the identification of bester, sterbe and best beluga hybrids, but also the identification of hybrids of unknown origin. This methodology has a good potential to contribute to the conservation of highly threatened sturgeon populations and also to the traceability of their products. |
format |
Text |
author |
Dudu, Andreea Samu, Maria Maereanu, Marilena Georgescu, Sergiu Emil |
author_facet |
Dudu, Andreea Samu, Maria Maereanu, Marilena Georgescu, Sergiu Emil |
author_sort |
Dudu, Andreea |
title |
A Multistep DNA-Based Methodology for Accurate Authentication of Sturgeon Species |
title_short |
A Multistep DNA-Based Methodology for Accurate Authentication of Sturgeon Species |
title_full |
A Multistep DNA-Based Methodology for Accurate Authentication of Sturgeon Species |
title_fullStr |
A Multistep DNA-Based Methodology for Accurate Authentication of Sturgeon Species |
title_full_unstemmed |
A Multistep DNA-Based Methodology for Accurate Authentication of Sturgeon Species |
title_sort |
multistep dna-based methodology for accurate authentication of sturgeon species |
publisher |
MDPI |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998085/ http://www.ncbi.nlm.nih.gov/pubmed/35407094 https://doi.org/10.3390/foods11071007 |
genre |
Acipenser baerii Beluga Beluga* |
genre_facet |
Acipenser baerii Beluga Beluga* |
op_source |
Foods |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998085/ http://www.ncbi.nlm.nih.gov/pubmed/35407094 http://dx.doi.org/10.3390/foods11071007 |
op_rights |
© 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
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https://doi.org/10.3390/foods11071007 |
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Foods |
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11 |
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7 |
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1007 |
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