Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica
Global warming has a strong impact on polar regions. Particularly, the Antarctic Peninsula and nearby islands have experienced a marked warming trend in the past 50 years. Therefore, higher methane (CH(4)) emissions from this area could be expected in the future. Since mitigation of these emissions...
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ftpubmed:oai:pubmedcentral.nih.gov:8969513 2023-05-15T13:38:52+02:00 Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica Roldán, Diego M. Carrizo, Daniel Sánchez-García, Laura Menes, Rodolfo Javier 2022-03-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969513/ https://doi.org/10.3389/fmicb.2022.822552 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969513/ http://dx.doi.org/10.3389/fmicb.2022.822552 Copyright © 2022 Roldán, Carrizo, Sánchez-García and Menes. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Microbiol Microbiology Text 2022 ftpubmed https://doi.org/10.3389/fmicb.2022.822552 2022-04-03T01:26:08Z Global warming has a strong impact on polar regions. Particularly, the Antarctic Peninsula and nearby islands have experienced a marked warming trend in the past 50 years. Therefore, higher methane (CH(4)) emissions from this area could be expected in the future. Since mitigation of these emissions can be carried out by microbial oxidation, understanding this biological process is crucial since to our knowledge, no related studies have been performed in this area before. In this work, the aerobic CH(4) oxidation potential of five freshwater lake sediments of Fildes Peninsula (King George Island, South Shetland Islands) was determined with values from 0.07 to 10 μmol CH(4) gdw(–1) day(–1) and revealed up to 100-fold increase in temperature gradients (5, 10, 15, and 20°C). The structure and diversity of the bacterial community in the sediments were analyzed by next-generation sequencing (Illumina MiSeq) of 16S rRNA and pmoA genes. A total of 4,836 ASVs were identified being Proteobacteria, Actinobacteriota, Acidobacteriota, and Bacteroidota the most abundant phyla. The analysis of the pmoA gene identified 200 ASVs of methanotrophs, being Methylobacter Clade 2 (Type I, family Methylococcaceae) the main responsible of the aerobic CH(4) oxidation. Moreover, both approaches revealed the presence of methanotrophs of the classes Gammaproteobacteria (families Methylococcaceae and Crenotrichaceae), Alphaproteobacteria (family Methylocystaceae), Verrucomicrobia (family Methylacidiphilaceae), and the candidate phylum of anaerobic methanotrophs Methylomirabilota. In addition, bacterial phospholipid fatty acids (PLFA) biomarkers were studied as a proxy for aerobic methane-oxidizing bacteria and confirmed these results. Methanotrophic bacterial diversity was significantly correlated with pH. In conclusion, our findings suggest that aerobic methanotrophs could mitigate in situ CH(4) emissions in a future scenario with higher temperatures in this climate-sensitive area. This study provides new insights into the diversity of ... Text Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands PubMed Central (PMC) Antarctic Antarctic Peninsula Fildes ENVELOPE(-58.817,-58.817,-62.217,-62.217) Fildes peninsula ENVELOPE(-58.948,-58.948,-62.182,-62.182) King George Island South Shetland Islands The Antarctic Frontiers in Microbiology 13 |
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English |
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Microbiology |
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Microbiology Roldán, Diego M. Carrizo, Daniel Sánchez-García, Laura Menes, Rodolfo Javier Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
topic_facet |
Microbiology |
description |
Global warming has a strong impact on polar regions. Particularly, the Antarctic Peninsula and nearby islands have experienced a marked warming trend in the past 50 years. Therefore, higher methane (CH(4)) emissions from this area could be expected in the future. Since mitigation of these emissions can be carried out by microbial oxidation, understanding this biological process is crucial since to our knowledge, no related studies have been performed in this area before. In this work, the aerobic CH(4) oxidation potential of five freshwater lake sediments of Fildes Peninsula (King George Island, South Shetland Islands) was determined with values from 0.07 to 10 μmol CH(4) gdw(–1) day(–1) and revealed up to 100-fold increase in temperature gradients (5, 10, 15, and 20°C). The structure and diversity of the bacterial community in the sediments were analyzed by next-generation sequencing (Illumina MiSeq) of 16S rRNA and pmoA genes. A total of 4,836 ASVs were identified being Proteobacteria, Actinobacteriota, Acidobacteriota, and Bacteroidota the most abundant phyla. The analysis of the pmoA gene identified 200 ASVs of methanotrophs, being Methylobacter Clade 2 (Type I, family Methylococcaceae) the main responsible of the aerobic CH(4) oxidation. Moreover, both approaches revealed the presence of methanotrophs of the classes Gammaproteobacteria (families Methylococcaceae and Crenotrichaceae), Alphaproteobacteria (family Methylocystaceae), Verrucomicrobia (family Methylacidiphilaceae), and the candidate phylum of anaerobic methanotrophs Methylomirabilota. In addition, bacterial phospholipid fatty acids (PLFA) biomarkers were studied as a proxy for aerobic methane-oxidizing bacteria and confirmed these results. Methanotrophic bacterial diversity was significantly correlated with pH. In conclusion, our findings suggest that aerobic methanotrophs could mitigate in situ CH(4) emissions in a future scenario with higher temperatures in this climate-sensitive area. This study provides new insights into the diversity of ... |
format |
Text |
author |
Roldán, Diego M. Carrizo, Daniel Sánchez-García, Laura Menes, Rodolfo Javier |
author_facet |
Roldán, Diego M. Carrizo, Daniel Sánchez-García, Laura Menes, Rodolfo Javier |
author_sort |
Roldán, Diego M. |
title |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_short |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_full |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_fullStr |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_full_unstemmed |
Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica |
title_sort |
diversity and effect of increasing temperature on the activity of methanotrophs in sediments of fildes peninsula freshwater lakes, king george island, antarctica |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969513/ https://doi.org/10.3389/fmicb.2022.822552 |
long_lat |
ENVELOPE(-58.817,-58.817,-62.217,-62.217) ENVELOPE(-58.948,-58.948,-62.182,-62.182) |
geographic |
Antarctic Antarctic Peninsula Fildes Fildes peninsula King George Island South Shetland Islands The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula Fildes Fildes peninsula King George Island South Shetland Islands The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands |
op_source |
Front Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969513/ http://dx.doi.org/10.3389/fmicb.2022.822552 |
op_rights |
Copyright © 2022 Roldán, Carrizo, Sánchez-García and Menes. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.822552 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
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1766111805666492416 |