Global gene expression responses of Atlantic salmon skin to Moritella viscosa
Moritella viscosa is a Gram-negative pathogen that causes large, chronic ulcers, known as winter-ulcer disease, in the skin of several fish species including Atlantic salmon. We used a bath challenge approach to profile the transcriptome responses of M. viscosa-infected Atlantic salmon skin at the l...
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ftpubmed:oai:pubmedcentral.nih.gov:8931016 2023-05-15T15:28:32+02:00 Global gene expression responses of Atlantic salmon skin to Moritella viscosa Eslamloo, Khalil Kumar, Surendra Xue, Xi Parrish, Kathleen S. Purcell, Sara L. Fast, Mark D. Rise, Matthew L. 2022-03-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931016/ http://www.ncbi.nlm.nih.gov/pubmed/35301338 https://doi.org/10.1038/s41598-022-08341-7 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931016/ http://www.ncbi.nlm.nih.gov/pubmed/35301338 http://dx.doi.org/10.1038/s41598-022-08341-7 © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Sci Rep Article Text 2022 ftpubmed https://doi.org/10.1038/s41598-022-08341-7 2022-03-27T01:35:30Z Moritella viscosa is a Gram-negative pathogen that causes large, chronic ulcers, known as winter-ulcer disease, in the skin of several fish species including Atlantic salmon. We used a bath challenge approach to profile the transcriptome responses of M. viscosa-infected Atlantic salmon skin at the lesion (Mv-At) and away from the lesion (Mv-Aw) sites. M. viscosa infection was confirmed through RNA-based qPCR assays. RNA-Seq identified 5212 and 2911 transcripts differentially expressed in the Mv-At compared to no-infection control and Mv-Aw groups, respectively. Also, there were 563 differentially expressed transcripts when comparing the Mv-Aw to control samples. Our results suggest that M. viscosa caused massive and strong, but largely infection site-focused, transcriptome dysregulations in Atlantic salmon skin, and its effects beyond the skin lesion site were comparably subtle. The M. viscosa-induced transcripts of Atlantic salmon were mainly involved in innate and adaptive immune response-related pathways, whereas the suppressed transcripts by this pathogen were largely connected to developmental and cellular processes. As validated by qPCR, M. viscosa dysregulated transcripts encoding receptors, signal transducers, transcription factors and immune effectors playing roles in TLR- and IFN-dependent pathways as well as immunoregulation, antigen presentation and T-cell development. This study broadened the current understanding of molecular pathways underlying M. viscosa-triggered responses of Atlantic salmon, and identified biomarkers that may assist to diagnose and combat this pathogen. Text Atlantic salmon PubMed Central (PMC) Scientific Reports 12 1 |
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Article Eslamloo, Khalil Kumar, Surendra Xue, Xi Parrish, Kathleen S. Purcell, Sara L. Fast, Mark D. Rise, Matthew L. Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
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description |
Moritella viscosa is a Gram-negative pathogen that causes large, chronic ulcers, known as winter-ulcer disease, in the skin of several fish species including Atlantic salmon. We used a bath challenge approach to profile the transcriptome responses of M. viscosa-infected Atlantic salmon skin at the lesion (Mv-At) and away from the lesion (Mv-Aw) sites. M. viscosa infection was confirmed through RNA-based qPCR assays. RNA-Seq identified 5212 and 2911 transcripts differentially expressed in the Mv-At compared to no-infection control and Mv-Aw groups, respectively. Also, there were 563 differentially expressed transcripts when comparing the Mv-Aw to control samples. Our results suggest that M. viscosa caused massive and strong, but largely infection site-focused, transcriptome dysregulations in Atlantic salmon skin, and its effects beyond the skin lesion site were comparably subtle. The M. viscosa-induced transcripts of Atlantic salmon were mainly involved in innate and adaptive immune response-related pathways, whereas the suppressed transcripts by this pathogen were largely connected to developmental and cellular processes. As validated by qPCR, M. viscosa dysregulated transcripts encoding receptors, signal transducers, transcription factors and immune effectors playing roles in TLR- and IFN-dependent pathways as well as immunoregulation, antigen presentation and T-cell development. This study broadened the current understanding of molecular pathways underlying M. viscosa-triggered responses of Atlantic salmon, and identified biomarkers that may assist to diagnose and combat this pathogen. |
format |
Text |
author |
Eslamloo, Khalil Kumar, Surendra Xue, Xi Parrish, Kathleen S. Purcell, Sara L. Fast, Mark D. Rise, Matthew L. |
author_facet |
Eslamloo, Khalil Kumar, Surendra Xue, Xi Parrish, Kathleen S. Purcell, Sara L. Fast, Mark D. Rise, Matthew L. |
author_sort |
Eslamloo, Khalil |
title |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_short |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_full |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_fullStr |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_full_unstemmed |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_sort |
global gene expression responses of atlantic salmon skin to moritella viscosa |
publisher |
Nature Publishing Group UK |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931016/ http://www.ncbi.nlm.nih.gov/pubmed/35301338 https://doi.org/10.1038/s41598-022-08341-7 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931016/ http://www.ncbi.nlm.nih.gov/pubmed/35301338 http://dx.doi.org/10.1038/s41598-022-08341-7 |
op_rights |
© The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-022-08341-7 |
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