Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts
Microorganisms have evolved to colonize all biospheres, including extremely cold environments, facing several stressor conditions, mainly low/freezing temperatures. In general, terms, the strategies developed by cold-adapted microorganisms include the synthesis of cryoprotectant and stress-protectan...
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ftpubmed:oai:pubmedcentral.nih.gov:8905146 2023-05-15T13:43:28+02:00 Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts Baeza, Marcelo Zúñiga, Sergio Peragallo, Vicente Gutierrez, Fernando Barahona, Salvador Alcaino, Jennifer Cifuentes, Víctor 2022-02-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905146/ https://doi.org/10.3389/fmicb.2022.828536 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905146/ http://dx.doi.org/10.3389/fmicb.2022.828536 Copyright © 2022 Baeza, Zúñiga, Peragallo, Gutierrez, Barahona, Alcaino and Cifuentes. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Microbiol Microbiology Text 2022 ftpubmed https://doi.org/10.3389/fmicb.2022.828536 2022-03-13T01:57:00Z Microorganisms have evolved to colonize all biospheres, including extremely cold environments, facing several stressor conditions, mainly low/freezing temperatures. In general, terms, the strategies developed by cold-adapted microorganisms include the synthesis of cryoprotectant and stress-protectant molecules, cold-active proteins, especially enzymes, and membrane fluidity regulation. The strategy could differ among microorganisms and concerns the characteristics of the cold environment of the microorganism, such as seasonal temperature changes. Microorganisms can develop strategies to grow efficiently at low temperatures or tolerate them and grow under favorable conditions. These differences can be found among the same kind of microorganisms and from the same cold habitat. In this work, eight cold-adapted yeasts isolated from King George Island, subAntarctic region, which differ in their growth properties, were studied about their response to low temperatures at the transcriptomic level. Sixteen ORFeomes were assembled and used for gene prediction and functional annotation, determination of gene expression changes, protein flexibilities of translated genes, and codon usage bias. Putative genes related to the response to all main kinds of stress were found. The total number of differentially expressed genes was related to the temperature variation that each yeast faced. The findings from multiple comparative analyses among yeasts based on gene expression changes and protein flexibility by cellular functions and codon usage bias raise significant differences in response to cold among the studied Antarctic yeasts. The way a yeast responds to temperature change appears to be more related to its optimal temperature for growth (OTG) than growth velocity. Yeasts with higher OTG prepare to downregulate their metabolism to enter the dormancy stage. In comparison, yeasts with lower OTG perform minor adjustments to make their metabolism adequate and maintain their growth at lower temperatures. Text Antarc* Antarctic King George Island PubMed Central (PMC) Antarctic King George Island Frontiers in Microbiology 13 |
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Microbiology |
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Microbiology Baeza, Marcelo Zúñiga, Sergio Peragallo, Vicente Gutierrez, Fernando Barahona, Salvador Alcaino, Jennifer Cifuentes, Víctor Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts |
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Microbiology |
description |
Microorganisms have evolved to colonize all biospheres, including extremely cold environments, facing several stressor conditions, mainly low/freezing temperatures. In general, terms, the strategies developed by cold-adapted microorganisms include the synthesis of cryoprotectant and stress-protectant molecules, cold-active proteins, especially enzymes, and membrane fluidity regulation. The strategy could differ among microorganisms and concerns the characteristics of the cold environment of the microorganism, such as seasonal temperature changes. Microorganisms can develop strategies to grow efficiently at low temperatures or tolerate them and grow under favorable conditions. These differences can be found among the same kind of microorganisms and from the same cold habitat. In this work, eight cold-adapted yeasts isolated from King George Island, subAntarctic region, which differ in their growth properties, were studied about their response to low temperatures at the transcriptomic level. Sixteen ORFeomes were assembled and used for gene prediction and functional annotation, determination of gene expression changes, protein flexibilities of translated genes, and codon usage bias. Putative genes related to the response to all main kinds of stress were found. The total number of differentially expressed genes was related to the temperature variation that each yeast faced. The findings from multiple comparative analyses among yeasts based on gene expression changes and protein flexibility by cellular functions and codon usage bias raise significant differences in response to cold among the studied Antarctic yeasts. The way a yeast responds to temperature change appears to be more related to its optimal temperature for growth (OTG) than growth velocity. Yeasts with higher OTG prepare to downregulate their metabolism to enter the dormancy stage. In comparison, yeasts with lower OTG perform minor adjustments to make their metabolism adequate and maintain their growth at lower temperatures. |
format |
Text |
author |
Baeza, Marcelo Zúñiga, Sergio Peragallo, Vicente Gutierrez, Fernando Barahona, Salvador Alcaino, Jennifer Cifuentes, Víctor |
author_facet |
Baeza, Marcelo Zúñiga, Sergio Peragallo, Vicente Gutierrez, Fernando Barahona, Salvador Alcaino, Jennifer Cifuentes, Víctor |
author_sort |
Baeza, Marcelo |
title |
Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts |
title_short |
Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts |
title_full |
Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts |
title_fullStr |
Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts |
title_full_unstemmed |
Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts |
title_sort |
response to cold: a comparative transcriptomic analysis in eight cold-adapted yeasts |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905146/ https://doi.org/10.3389/fmicb.2022.828536 |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic King George Island |
genre_facet |
Antarc* Antarctic King George Island |
op_source |
Front Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905146/ http://dx.doi.org/10.3389/fmicb.2022.828536 |
op_rights |
Copyright © 2022 Baeza, Zúñiga, Peragallo, Gutierrez, Barahona, Alcaino and Cifuentes. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.828536 |
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Frontiers in Microbiology |
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13 |
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1766189450104143872 |