Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus
Laboratory investigations on terrestrial model-species, typically of temperate origin, have demonstrated that terrestrial ectotherms can cope with daily temperature variations through rapid hardening responses. However, few studies have investigated this ability and its physiological basis in the fi...
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ftpubmed:oai:pubmedcentral.nih.gov:8889080 2023-05-15T15:13:17+02:00 Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus Noer, Natasja Krog Sørensen, Mathias Hamann Colinet, Hervé Renault, David Bahrndorff, Simon Kristensen, Torsten Nygaard 2022-02-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889080/ https://doi.org/10.3389/fphys.2022.818485 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889080/ http://dx.doi.org/10.3389/fphys.2022.818485 Copyright © 2022 Noer, Sørensen, Colinet, Renault, Bahrndorff and Kristensen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Physiol Physiology Text 2022 ftpubmed https://doi.org/10.3389/fphys.2022.818485 2022-03-06T02:06:00Z Laboratory investigations on terrestrial model-species, typically of temperate origin, have demonstrated that terrestrial ectotherms can cope with daily temperature variations through rapid hardening responses. However, few studies have investigated this ability and its physiological basis in the field. Especially in polar regions, where the temporal and spatial temperature variations can be extreme, are hardening responses expected to be important. Here, we examined diurnal adjustments in heat and cold tolerance in the Greenlandic seed bug Nysius groenlandicus by collecting individuals for thermal assessment at different time points within and across days. We found a significant correlation between observed heat or cold tolerance and the ambient microhabitat temperatures at the time of capture, indicating that N. groenlandicus continuously and within short time-windows respond physiologically to thermal changes and/or other environmental variables in their microhabitats. Secondly, we assessed underlying metabolomic fingerprints using GC-MS metabolomics in a subset of individuals collected during days with either low or high temperature variation. Concentrations of metabolites, including sugars, polyols, and free amino acids varied significantly with time of collection. For instance, we detected elevated sugar levels in animals caught at the lowest daily field temperatures. Polyol concentrations were lower in individuals collected in the morning and evening and higher at midday and afternoon, possibly reflecting changes in temperature. Additionally, changes in concentrations of metabolites associated with energetic metabolism were observed across collection times. Our findings suggest that in these extreme polar environments hardening responses are marked and likely play a crucial role for coping with microhabitat temperature variation on a daily scale, and that metabolite levels are actively altered on a daily basis. Text Arctic greenlandic PubMed Central (PMC) Arctic Frontiers in Physiology 13 |
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Physiology Noer, Natasja Krog Sørensen, Mathias Hamann Colinet, Hervé Renault, David Bahrndorff, Simon Kristensen, Torsten Nygaard Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
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Physiology |
description |
Laboratory investigations on terrestrial model-species, typically of temperate origin, have demonstrated that terrestrial ectotherms can cope with daily temperature variations through rapid hardening responses. However, few studies have investigated this ability and its physiological basis in the field. Especially in polar regions, where the temporal and spatial temperature variations can be extreme, are hardening responses expected to be important. Here, we examined diurnal adjustments in heat and cold tolerance in the Greenlandic seed bug Nysius groenlandicus by collecting individuals for thermal assessment at different time points within and across days. We found a significant correlation between observed heat or cold tolerance and the ambient microhabitat temperatures at the time of capture, indicating that N. groenlandicus continuously and within short time-windows respond physiologically to thermal changes and/or other environmental variables in their microhabitats. Secondly, we assessed underlying metabolomic fingerprints using GC-MS metabolomics in a subset of individuals collected during days with either low or high temperature variation. Concentrations of metabolites, including sugars, polyols, and free amino acids varied significantly with time of collection. For instance, we detected elevated sugar levels in animals caught at the lowest daily field temperatures. Polyol concentrations were lower in individuals collected in the morning and evening and higher at midday and afternoon, possibly reflecting changes in temperature. Additionally, changes in concentrations of metabolites associated with energetic metabolism were observed across collection times. Our findings suggest that in these extreme polar environments hardening responses are marked and likely play a crucial role for coping with microhabitat temperature variation on a daily scale, and that metabolite levels are actively altered on a daily basis. |
format |
Text |
author |
Noer, Natasja Krog Sørensen, Mathias Hamann Colinet, Hervé Renault, David Bahrndorff, Simon Kristensen, Torsten Nygaard |
author_facet |
Noer, Natasja Krog Sørensen, Mathias Hamann Colinet, Hervé Renault, David Bahrndorff, Simon Kristensen, Torsten Nygaard |
author_sort |
Noer, Natasja Krog |
title |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_short |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_full |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_fullStr |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_full_unstemmed |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_sort |
rapid adjustments in thermal tolerance and the metabolome to daily environmental changes – a field study on the arctic seed bug nysius groenlandicus |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889080/ https://doi.org/10.3389/fphys.2022.818485 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic greenlandic |
genre_facet |
Arctic greenlandic |
op_source |
Front Physiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889080/ http://dx.doi.org/10.3389/fphys.2022.818485 |
op_rights |
Copyright © 2022 Noer, Sørensen, Colinet, Renault, Bahrndorff and Kristensen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fphys.2022.818485 |
container_title |
Frontiers in Physiology |
container_volume |
13 |
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