Differential DNA methylation in Black and White individuals with chronic low back pain enrich different genomic pathways

Compared to Non-Hispanic Whites (NHWs), individuals who self-identify as Non-Hispanic Blacks (NHBs) in the United States experience more severe and disabling chronic low back pain (cLBP). We hypothesized that differences in DNA methylation (DNAm) play a role in racial disparities in cLBP. PURPOSE: T...

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Published in:Neurobiology of Pain
Main Authors: Aroke, Edwin N., Jackson, Pamela, Meng, Lingsong, Huo, Zhiguang, Overstreet, Demario S., Penn, Terence M., Quinn, Tammie L., Cruz-Almeida, Yenisel, Goodin, Burel R.
Format: Text
Language:English
Published: Elsevier 2022
Subjects:
DML
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885563/
https://doi.org/10.1016/j.ynpai.2022.100086
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spelling ftpubmed:oai:pubmedcentral.nih.gov:8885563 2023-05-15T16:02:05+02:00 Differential DNA methylation in Black and White individuals with chronic low back pain enrich different genomic pathways Aroke, Edwin N. Jackson, Pamela Meng, Lingsong Huo, Zhiguang Overstreet, Demario S. Penn, Terence M. Quinn, Tammie L. Cruz-Almeida, Yenisel Goodin, Burel R. 2022-02-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885563/ https://doi.org/10.1016/j.ynpai.2022.100086 en eng Elsevier http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885563/ http://dx.doi.org/10.1016/j.ynpai.2022.100086 © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). CC-BY-NC-ND Neurobiol Pain Original Research Article Text 2022 ftpubmed https://doi.org/10.1016/j.ynpai.2022.100086 2022-03-06T02:00:19Z Compared to Non-Hispanic Whites (NHWs), individuals who self-identify as Non-Hispanic Blacks (NHBs) in the United States experience more severe and disabling chronic low back pain (cLBP). We hypothesized that differences in DNA methylation (DNAm) play a role in racial disparities in cLBP. PURPOSE: To determine the relationship between DNAm levels and racial group differences in adults with cLBP. Our study’s secondary purpose was to perform a race-stratified comparison of adults with cLBP and pain-free controls and identify functional genomic pathways enriched by annotated differentially methylated genes. PATIENTS AND METHODS: We recruited 49 NHBs and 49 NHWs (49 cLBP and 49 pain-free controls, PFCs), analyzed DNAm from whole blood using reduced representation bisulfite sequencing, and identified enriched genomic pathways. RESULTS: Among participants with cLBP, we identified 2873 differentially methylated loci (DML; methylation differences of at least 10% and p < 0.0001), many of which were annotated to genes of importance to pain pathology. These DMLs significantly enriched pathways to involved in nociception/pain processing (Dopamine-DARPP32 Feedback in cAMP signaling, GABA Receptor Signaling, Opioid Signaling) and neuronal differentiation (e.g., Calcium Signaling, Axon Guidance Signaling, and Endocannabinoid Neuronal Synapse). Our race stratified analyses of individuals with cLBP versus PFCs revealed 2356 DMLs in NHBs and 772 DMLs in NHWs with p < 0.0001 and > 10% methylation difference. Ingenuity Pathway Analysis revealed that many pathways of significance to pain such as Corticotropin Releasing Hormone Signaling, White Adipose Tissue Browning, and GABA Receptor Signaling pathways, were more significant in NHBs than NHWs. CONCLUSION: Even though an individual’s self-identified race is a social construct, not a biological variable, racism associated with that classification affects virtually every aspect of life, including disease risk. DNAm induced alterations in stress signaling pathways may explain ... Text DML PubMed Central (PMC) Browning ENVELOPE(164.050,164.050,-74.617,-74.617) Neurobiology of Pain 11 100086
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Original Research Article
spellingShingle Original Research Article
Aroke, Edwin N.
Jackson, Pamela
Meng, Lingsong
Huo, Zhiguang
Overstreet, Demario S.
Penn, Terence M.
Quinn, Tammie L.
Cruz-Almeida, Yenisel
Goodin, Burel R.
Differential DNA methylation in Black and White individuals with chronic low back pain enrich different genomic pathways
topic_facet Original Research Article
description Compared to Non-Hispanic Whites (NHWs), individuals who self-identify as Non-Hispanic Blacks (NHBs) in the United States experience more severe and disabling chronic low back pain (cLBP). We hypothesized that differences in DNA methylation (DNAm) play a role in racial disparities in cLBP. PURPOSE: To determine the relationship between DNAm levels and racial group differences in adults with cLBP. Our study’s secondary purpose was to perform a race-stratified comparison of adults with cLBP and pain-free controls and identify functional genomic pathways enriched by annotated differentially methylated genes. PATIENTS AND METHODS: We recruited 49 NHBs and 49 NHWs (49 cLBP and 49 pain-free controls, PFCs), analyzed DNAm from whole blood using reduced representation bisulfite sequencing, and identified enriched genomic pathways. RESULTS: Among participants with cLBP, we identified 2873 differentially methylated loci (DML; methylation differences of at least 10% and p < 0.0001), many of which were annotated to genes of importance to pain pathology. These DMLs significantly enriched pathways to involved in nociception/pain processing (Dopamine-DARPP32 Feedback in cAMP signaling, GABA Receptor Signaling, Opioid Signaling) and neuronal differentiation (e.g., Calcium Signaling, Axon Guidance Signaling, and Endocannabinoid Neuronal Synapse). Our race stratified analyses of individuals with cLBP versus PFCs revealed 2356 DMLs in NHBs and 772 DMLs in NHWs with p < 0.0001 and > 10% methylation difference. Ingenuity Pathway Analysis revealed that many pathways of significance to pain such as Corticotropin Releasing Hormone Signaling, White Adipose Tissue Browning, and GABA Receptor Signaling pathways, were more significant in NHBs than NHWs. CONCLUSION: Even though an individual’s self-identified race is a social construct, not a biological variable, racism associated with that classification affects virtually every aspect of life, including disease risk. DNAm induced alterations in stress signaling pathways may explain ...
format Text
author Aroke, Edwin N.
Jackson, Pamela
Meng, Lingsong
Huo, Zhiguang
Overstreet, Demario S.
Penn, Terence M.
Quinn, Tammie L.
Cruz-Almeida, Yenisel
Goodin, Burel R.
author_facet Aroke, Edwin N.
Jackson, Pamela
Meng, Lingsong
Huo, Zhiguang
Overstreet, Demario S.
Penn, Terence M.
Quinn, Tammie L.
Cruz-Almeida, Yenisel
Goodin, Burel R.
author_sort Aroke, Edwin N.
title Differential DNA methylation in Black and White individuals with chronic low back pain enrich different genomic pathways
title_short Differential DNA methylation in Black and White individuals with chronic low back pain enrich different genomic pathways
title_full Differential DNA methylation in Black and White individuals with chronic low back pain enrich different genomic pathways
title_fullStr Differential DNA methylation in Black and White individuals with chronic low back pain enrich different genomic pathways
title_full_unstemmed Differential DNA methylation in Black and White individuals with chronic low back pain enrich different genomic pathways
title_sort differential dna methylation in black and white individuals with chronic low back pain enrich different genomic pathways
publisher Elsevier
publishDate 2022
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885563/
https://doi.org/10.1016/j.ynpai.2022.100086
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op_source Neurobiol Pain
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885563/
http://dx.doi.org/10.1016/j.ynpai.2022.100086
op_rights © 2022 The Author(s)
https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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